Abstract
The present study describes a novel microcarrier substrate consisting of a swellable, copolymer of styrene and divinylbenzene, derivatized with trimethylamine. The co-polymer trimethylamine microcarriers support the growth of a number of different cell lines — Madin Darby Bovine Kidney, Madin-Darby Canine Kidney, Vero and Cos-7 — under serum-free conditions, and human diploid fibroblasts in serum-containing medium. Cells attach to the co-polymer trimethylamine microcarriers as rapidly as they attach to other charged-surface microcarriers (faster than they attach to collagen-coated polystyrene microcarriers) and spread rapidly after attachment. All of the cells examined grow to high density on the co-polymer trimethylamine microcarriers. Furthermore, cells are readily released from the surface after exposure to a solution of trypsin/EDTA. In this respect, the co-polymer trimethylamine microcarriers are different from other charged-surface microcarriers. Madin-Darby Bovine Kidney cells grown on this substrate support production of vaccine strain infectious bovine rhinotracheitis virus as readily as on other charged-surface or collagen-coated microcarriers. Thus, the co-polymer trimethylamine microcarriers combine the positive characteristics of the currently available charged-surface and adhesion-peptide coated microcarriers in a single product. The viral vaccine production industry is undergoing considerable change as manufacturers move toward complete, animal product-free culture systems. This novel substrate should find application in the industry, especially in processes which depend on viable cell recovery.
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Abbreviations
- MDBK:
-
Madin Darby Bovine Kidney Cells
- MDCK:
-
Madin Darby Canine Kidney Cells
- CPTMA:
-
Co-polymer trimethylamine
- DMEM-FBS:
-
Dulbecco’s Modified Medium Essential Medium of Eagle with 10% fetal bovine serum
- IBR:
-
Infectious bovine rhinotracheities virus
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© 1998 Springer Science+Business Media Dordrecht
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Varani, J., Piel, F., Josephs, S., Beals, T.F., Hillegas, W.J. (1998). Attachment and growth of anchorage-dependent cells on a novel, charged-surface microcarrier under serum-free conditions. In: Betenbaugh, M.J., Chalmers, J.J., Arathoon, R., Chaplen, F.W.R., Mastrangelo, A.J. (eds) Cell Culture Engineering VI. Current Applications of Cell Culture Engineering, vol 3. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4786-6_12
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DOI: https://doi.org/10.1007/978-94-011-4786-6_12
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