Somatic Embryogenesis in Tea (Camellia sinensis (L.) O. Kuntze)

  • A. Akula
  • C. Akula
Part of the Forestry Sciences book series (FOSC, volume 59)


Tea (Camellia sinensis (L.) O. Kuntze) is the oldest caffeine-containing beverage; it supposedly originated from China and North Eastern region of India. From the earliest times tea was known for its properties as a healthy, refreshing drink and the word ch’a was used in China and India. By the third century AD many stories were being told and some written about tea and the benefits of tea drinking, but it was not until the Tang dynasty (618–907 AD) that tea become China’s national drink. Arab traders first introduced tea to Europe in 1559. However, it is the Portuguese and Dutch who claim the credit of bringing tea to Europe. During the late 17th century tea used to be drunk by aristocratic families only. By the middle of the 18th century, however, tea had replaced ale and gin as the drink of the masses and had become Britain’s most popular beverage. Today, tea has become the most popular and cheapest drink in the world. More than half of the world’s population is habituated of tea drinking. India, Sri Lanka, Indonesia, China and Japan are the traditional tea producers in the world. The demand for tea is ever increasing, and more and more land is being brought under tea plantations. Tea is currently cultivated in more than 50 countries, including Zimbabwe, Kenya, Tanzania, Iran, Turkey, Malawi, Iran, Afghanistan, Bangladesh, Russia, New Zealand, Papua New Guinea and Australia.


Somatic Embryo Somatic Embryogenesis Zygotic Embryo Camellia Sinensis Immature Zygotic Embryo 
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  1. Agarwal, B., U. Singh and M. Banerjee. 1992 In vitro clonal propagation of tea (Camellia sinensis (L) O. Kuntze). Plant Cell Tiss Org Cult. 30: 1–6.CrossRefGoogle Scholar
  2. Akula, A. and W.A. Dodd. 1998. Direct somatic embryogenesis in a selected tea clone, TRI 2025, Camellia sinensis from nodal expiants. Plant Cell Rep. 17: 804–809.CrossRefGoogle Scholar
  3. Arulpragasm, P.V. and R. Latiff. 1986. Studies on the tissue culture of tea (Camellia sinensis (L) O. Kuntze): 1. Development of a culture method for the multiplication of shoots. Sri Lanka J Tea Sci. 55: 44–47.Google Scholar
  4. Arulpragasm, P.V., R. Latiff and P. Seneviratne. 1988. Studies on the tissue culture of tea (Camellia sinensis (L.) O. Kuntze): 3. Regeneration of plants from cotyledon callus culture. Sri Lanka J Tea Sci. 57: 20–23.Google Scholar
  5. Bano, Z., S. Rajarathnam and B.D. Mohanty. 1991. Somatic embryogenesis in cotyledon culture of tea (Thea sinensis L.). J Hort Sci. 66: 465–470.Google Scholar
  6. Bezbaruah, H.P. 1984. A revised method for selection clones. Two and a Bud. 31: 13–16.Google Scholar
  7. Bonheure, D. 1990. Botany and ecology. In: Tea, pp. 2–7. (ed. R. Coste). London: Macmillan.Google Scholar
  8. Chen, Z. and H. Lio. 1990. A success in bringing out tea plant from the anthers. China Tea 5:6–7.Google Scholar
  9. Dodd, W.A. 1994. Tissue culture of tea (Camellia sinensis (L) O. Kuntze). A review. Int J Trop Agr. 12: 212–247.Google Scholar
  10. Jefferson, R.A. 1987. Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep. 5: 387–405.CrossRefGoogle Scholar
  11. Jain, S.M. and R.J. Newton. 1990. Prospects of biotechnology for tea improvement. Proc Ind Nat Sci Acad. B56: 441–448.Google Scholar
  12. Jha, T.B. and S. Sen. 1992. Micropropagation of an elite Darjeeling tea clone. Plant Cell Rep. 11: 101–104.CrossRefGoogle Scholar
  13. Kato, M. 1986a. Micropropagation through cotyledon culture in Camellia japonica L. and C. sinensis. Jpn J Breed. 36: 31–33.Google Scholar
  14. Kato, M. 1986b. Micropropagation through cotyledon culture in Camellia sinensis. Jpn J Breed. 36: 82–83.Google Scholar
  15. Kato, M. 1988. The regeneration in different leaf stages of tea leaf culture. Jpn J Breed. 38: 92–93.Google Scholar
  16. Kato, M. 1989. Camellia sinensis L. (Tea). In Vitro regeneration. In: Biotechnology in Agriculture and Forestry. Vol. 7, pp. 82–98. (eds. Bajaj, Y.P.S.) Berlin Heidelberg: Springer-Verlag.Google Scholar
  17. Kato, M. 1996. Somatic embryogenesis from immature leaves of in vitro frown tea shoots. Plant Cell Rep. 15: 920–923.CrossRefGoogle Scholar
  18. Kleinschmit, J. and A. Meier-Dinkel. 1990. Biotechnology in forest tree improvement: trees of the future. In: Plant Aging and Applied Approaches. NATO ASI Series, Series A: Life Sci Vol. 186, pp. 319–323. (eds. Rodriguez R., R.S. Tames and DJ. Durzan). New York: Plenum Press.Google Scholar
  19. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bio assays with tobacco tissue culture. Physiol Plant. 15: 473–497.CrossRefGoogle Scholar
  20. Nakamura, Y. 1987a. Shoot tip culture of tea cultivar Yabukita. Tea Res J. 65: 1–7.CrossRefGoogle Scholar
  21. Nakamura, Y. 1987b. In vitro rapid plantlet culture from axillary buds of tea plants (Camellia sinensis (L.) O. Kuntze). Bull Shizuoka Tea Exp St. 13: 23–27.Google Scholar
  22. Nakamura, Y. 1988a. Efficient differentiation of adventitious embryos from cotyledon culture of Camellia sinensis and other Camellia species. Tea Res J. 67: 1–12.Google Scholar
  23. Nakamura, Y. 1988b. Effective methods of in vitro propagation of tea plant. Proc In Symp. On Recent Devel Tea Prod. pp. 63–75.Google Scholar
  24. Nakamura, Y. 1991. In vitro propagation techniques of tea plants. Jpn Agric Res Q. 25: 185–194.Google Scholar
  25. Nakamura, Y and M. Shibata. 1990. Micropropagation of tea plant (Camellia sinensis (L.) O. Kuntze) through in vitro cuttings. Effects of various hormones on growth of shoots from axillary buds. Tea Res J. 72: 9–17.CrossRefGoogle Scholar
  26. Pierik, R.L.M. 1990. Rejuvenation and micropropagation. In: Progress in Plant Cellular and Molecular Biology. pp. 91–101. (eds. H.J.J. Nijkamp, L.H.W. Plas and J. Aartrijk). Dordrecht: Kluwer Academic Publishers.CrossRefGoogle Scholar
  27. Ponsamuel, J., N. Samson, P. Ganeshan, V. Sathyaprakash and G. Abraham. 1996. Somatic embryogenesis and plant regeneration from the immature cotyledonary tissues of cultivated tea (Camellia sinensis (L). O. Kuntze). Plant Cell Rep. 16: 210–214.CrossRefGoogle Scholar
  28. Sarathchandra, T.M., P.D. Upali and P.V. Arulpragasam. 1990. Progress towards the commercial propagation of tea by tissue culture techniques. Sri Lanka J Tea Sci. 59: 62–64.Google Scholar
  29. Shimokado, T., T. Murata and Y. Miyaji]. 1986. Formation of embryoid by anther culture of tea. Jpn J Breed. 36: 282–283Google Scholar
  30. Sivapalan, P. 1988. An assessment of the status of productivity of tea in Sri Lanka. Tea Proc Regional Tea (Scientific) Conference. Sri Lanka.Google Scholar
  31. Tunstall, A. 1931. A note on the propagation of tea by green shoot cuttings. Quart J Indian Tea Assoc. 49–51.Google Scholar
  32. Van Doorne, L.E., G. Marshall and R.C. Kirkwood. 1995. Somatic embryogenesis in pea (Pisum sativum L.); effect of expiant, genotype and culture conditions. Ann Appl Biol. 126: 169–179.CrossRefGoogle Scholar
  33. Wachira, F. and J. Ogada. 1995. In vitro regeneration of Camellia sinensis (L.) O. Kuntze by somatic embryogenesis. Plant Cell Rep. 14: 463–466.CrossRefGoogle Scholar
  34. Wight, W. 1956. Commercial selection and breeding of tea in India. World Crop. 8: 263–268.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1999

Authors and Affiliations

  • A. Akula
    • 1
  • C. Akula
    • 1
  1. 1.Centre for Plant Conservation GeneticsSouthern Cross UniversityLismoreAustralia

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