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Micro-Mechanical Measurement of the Torsional Modulus of DNA

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Structural Biology and Functional Genomics

Part of the book series: NATO Science Series ((ASHT,volume 71))

Abstract

The torsional modulus C of DNA is determined from the difference between the work of stretching a single overwound molecule and the work done stretching one underwound by the same number of turns. The value obtained C/k B T = 86 ± 10 nm is within the range (75 ± 25 nm) estimated by more indirect methods

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References

  1. J.H. White. Self linking and the Gauss integral in higher dimensions. Am. J. Math, 91:693–728, 1969.

    Article  Google Scholar 

  2. T. Strick, J.-F. Allemand, D. Bensimon, and V. Croquette. The behavior of supercoiled DNA. Biophys. J, 74:2016–2028, 1998.

    Article  PubMed  CAS  Google Scholar 

  3. P. Cluzel, A. Lebrun, C. Heller, R. Lavery, J.-L. Viovy, D. Chatenay, and F. Caron.DNA: an extensible molecule. Science, 271:792–794, 1996.

    Article  PubMed  CAS  Google Scholar 

  4. S.B. Smith, Y. Cui, and C. Bustamante. Overstretching B-DNA: the elastic response of individual double-stranded and single-stranded DNA molecules. Science, 271:795–799, 1996.

    Article  PubMed  CAS  Google Scholar 

  5. C. Bustamante, J.F. Marko, E.D. Siggia, and S. Smith. Entropie elasticity of X-phage DNA. Science, 265:1599–1600, 1994.

    Article  PubMed  CAS  Google Scholar 

  6. J.F. Marko and E. Siggia. Statistical mechanics of supercoiled DNA. Phys. Rev. E, 52(3):2912–2938, september 95.

    Google Scholar 

  7. J.F. Marko and E.D. Siggia. Fluctuations and supercoiling of DNA. Science, 265:506–508,1994.

    Article  PubMed  CAS  Google Scholar 

  8. P.J. Hagerman. Flexibility of DNA. Ann. Rev. Biophys. Biophys. Chem, 17:265–268, 1988.

    Article  CAS  Google Scholar 

  9. C. Bouchiat, M.D. Wang, S. M. Block, J.-F. Allemand, and V. Croquette;. Measuring persitence length of a single DNA molecule. Biophys. J, in press, 1998.

    Google Scholar 

  10. T. Strick, J.F. Allemand, D. Bensimon, A. Bensimon, and V. Croquette. The elasticity of a single supercoiled DNA molecule. Science, 271:1835–1837, 1996.

    Article  PubMed  CAS  Google Scholar 

  11. J.-F. Allemand, D. Bensimon, R. Lavery, and V. Croquette.Stretched and overwound DNA form a Pauling-like structure with exposed bases. Proc. Nat. Acad. Sci. USA, in press, 1998.

    Google Scholar 

  12. J.D. Moroz and P. Nelson. Torsional directed walks, entropie elasticity and DNA twist stiffness. Proc. Nat. Acad. Sci. USA, 94:14418–14422, 1997.

    Article  PubMed  CAS  Google Scholar 

  13. C. Bouchiat and M. Mézard. Elasticity theory of a supercoiled DNA molecules. Phys.Rev. Lett, 80:1556–1559, 1997.

    Article  Google Scholar 

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© 1999 Springer Science+Business Media Dordrecht

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Strick, T.R., Bensimon, D., Croquette, V. (1999). Micro-Mechanical Measurement of the Torsional Modulus of DNA. In: Bradbury, E.M., Pongor, S. (eds) Structural Biology and Functional Genomics. NATO Science Series, vol 71. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4631-9_5

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  • DOI: https://doi.org/10.1007/978-94-011-4631-9_5

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-0-7923-5782-7

  • Online ISBN: 978-94-011-4631-9

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