Abstract
A screening via mRNA differential display resulted in the identification and cloning of nine partial cDNAs corresponding to putative peach fruitlet abscission-related genes. Five of the nine genes were induced by propylene mediated abscission and four were specifically repressed during this process. None of the isolated genes were specifically induced only in the abscission layer, while at least two corresponded to mRNAs which accumulate in adjacent tissues. DNA sequence analysis demonstrated that two cDNAs show homology to major latex and β 1,3-glucanase genes. The major latex-like gene is specifically expressed only in pedicels prior to abscission and is down-regulated by propylene. The β 1,3-glucanase transcript accumulation is detected in all examined tissues following propylene treatment. The major latex protein and β 1,3-glucanase genes have been implicated in abscission-related wound healing and plant pathogen defense, respectively.
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Ramina, A., Bonghi, C., Giovannoni, J.J., Ruperti, B., Tonutti, P. (1999). Differential Display and Isolation of cDNAS Corresponding to mRNAS Whose Abundance is Influenced by Ethylene During Peach Fruitlet Abscission. In: Kanellis, A.K., Chang, C., Klee, H., Bleecker, A.B., Pech, J.C., Grierson, D. (eds) Biology and Biotechnology of the Plant Hormone Ethylene II. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4453-7_44
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DOI: https://doi.org/10.1007/978-94-011-4453-7_44
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