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Cloning of Tomato DAD1 and Study of its Expression During Programmed Cell Death and Fruit Ripening

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Biology and Biotechnology of the Plant Hormone Ethylene II

Abstract

The dadl gene product is involved in suppression of programmed cell death during Caenorhabditis elegans embryogenesis. Homologues have been cloned from several animal and plant species. We isolated a dadl cDNA clone from tomato and found that the predicted gene product shows significant homology with various DAD1 proteins. Northern analysis showed that, during camptothecin-induced programmed cell death in tomato suspension cells, dadl mRNA levels did not show the expected decrease. During tomato fruit ripening, expression levels in pericarp tissue increased or decreased, depending on the tomato variety.

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© 1999 Springer Science+Business Media Dordrecht

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Hoeberichts, F.A., Van Der Plas, L.H.W., Woltering, E.J. (1999). Cloning of Tomato DAD1 and Study of its Expression During Programmed Cell Death and Fruit Ripening. In: Kanellis, A.K., Chang, C., Klee, H., Bleecker, A.B., Pech, J.C., Grierson, D. (eds) Biology and Biotechnology of the Plant Hormone Ethylene II. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4453-7_39

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  • DOI: https://doi.org/10.1007/978-94-011-4453-7_39

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5910-7

  • Online ISBN: 978-94-011-4453-7

  • eBook Packages: Springer Book Archive

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