Abstract
We are using molecular genetic approaches in Arabidopsis thaliana in order to elucidate the mechanisms of ethylene signal transduction. In particular, we are interested in understanding how the ETR1 ethylene receptor and its homologs signal to the downstream protein kinase, CTR1. A starting point for addressing this question is to examine protein-protein interactions involving the receptors and CTRl. To detect protein interactions, we are utilizing the yeast two-hybrid assay as well as in vitro protein association assays. Using these methods, we are analyzing the association between the putative regulatory domain of CTR1 and the predicted cytoplasmic portions of both ETR1 and ERS1. In addition, we have been screening yeast two-hybrid libraries in order to identify proteins that interact with the CTR1 amino-terminal domain and/or the ETR1, ETR2 and ERS1 ethylene receptors.
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References
Ecker, J.R. (1995) The ethylene signal transduction pathway in plants, Science 268, 667–674.
Kieber, J.J., Rothenberg, M., Roman, G., Feldmann, K.A., and Ecker, J.R. (1993) CTR1, a negative regulator of the ethylene response pathway in Arabidopsis, encodes a member of the raf family of protein kinases, Cell 72, 427–441.
Roman, G., Lubarsky, B., Kieber, J.J., Rothenberg, M., and Ecker, J.R. (1995) Genetic analysis of ethylene signal transduction in Arabidopsis thaliana: Five novel mutant loci integrated into a stress response pathway, Genetics 139, 1393–1409.
Parkinson, J.S. and Kofoid, E.C. (1992) Communication modules in bacterial signaling proteins, Annu. Rev. Genet. 26, 71–112.
Schaller, E.G. and Bleecker, A.B. (1995) Ethylene binding sites generated in yeast expressing the Arabidopsis ETR1 gene, Science 270, 1809–1811.
Gamble R.L., Coonfield M.L., and Schaller G.E. (1998) Histidine kinase activity of the ETR1 ethylene receptor from Arabidopsis, Proc. Natl. Acad. Sci. USA 95, 7825–7829.
Bleecker, A.B., Estelle, M.A., Somerville, C., and Kende, H. (1988) Insensitivity to ethylene conferred by a dominant mutation in Arabidopsis thaliana, Science 241, 1086–1089.
Hua, J. Sakai, H., Nourizadeh, S., Chen, Q.G., Bleecker, A. B., Ecker, J. R., and Meyerowitz, E. M. (1998) EIN4 and ERS2 are members of the putative ethylene receptor gene family in Arabidopsis, Plant Cell 10, 1321–1332.
Sakai, H., Hua, J., Chen, Q.G., Chang, C., Medrano, L.J., Bleecker, A.B., and Meyerowitz, E.M. (1998) ETR2 is an ETRI-like gene involved in ethylene signaling in Arabidopsis, Proc. Natl. Acad. Sci. USA 95, 5812–5817.
Hua, J. and Meyerowitz, E.M. (1998) Ethylene responses are negatively regulated by a receptor gene family in Arabidopsis thaliana, Cell 94, 261–271.
Fields, S. and Sternglanz, R. (1994) The two-hybrid system: an assay for protein-protein interactions, Trends Genet. 10, 286–292.
Clark K.L., Larsen P.B., Wang X., and Chang C. (1998) Association of the Arabidopsis CTR1 Raflike kinase with the ETR1 and ERS ethylene receptors, Proc. Natl. Acad. Sci. USA 95, 5401–5406.
Posas, F. and Saito, H. (1998) Activation of the yeast SSK2 MAP kinase kinase kinase by the SSK1 two-component response regulator, EMBO J. 17, 1385–1394.
Posas, F., Wurgler-Murphy, S.M., Maeda, T., Witten, E.A., Thai, T.C., and Saito, H. (1996) Yeast HOG1 MAP kinase cascade is regulated by a multi-step phosphorelay mechanism in the SLN1-YPD1-SSK1 “two-component” osmosensor, Cell 86, 865–875.
Fantl, W.J., Muslin, A. J., Kikuchi, A., Martin, J.A., MacNicol, A.M., Gross, R.W., and Williams, L.T. (1994) Activation of Raf-1 by 14-3-3 proteins, Nature 371, 612–614.
Freed, E., Symons, M., Macdonald, S.G., McCormick, F., and Ruggieri, R. (1994) Binding of 14-3-3 proteins to the protein kinase Raf and effects on its activation, Science 265, 1713–1716.
Fu, H., Xia, K., Pallas, D.C., Cui, C. Conroy, K., Narsimhan, R.P., Mamon, H., Collier, R.J., and Roberts, T.M. (1994) Interaction of the protein kinase Raf-1 with the 14-3-3 proteins, Science 266, 126–129.
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© 1999 Springer Science+Business Media Dordrecht
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Chang, C. et al. (1999). Protein-Protein Interactions in Ethylene Signal Transduction in Arabidopsis. In: Kanellis, A.K., Chang, C., Klee, H., Bleecker, A.B., Pech, J.C., Grierson, D. (eds) Biology and Biotechnology of the Plant Hormone Ethylene II. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4453-7_13
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DOI: https://doi.org/10.1007/978-94-011-4453-7_13
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