Abstract
Differentiation of archeospores was observed from excised tissue of young thalli of various monoecious Porphyra species (P tenera, P. yezoensis, P suborbiculata, P okamurae) after 4–8 days in culture at temperatures of 20 and 25 °C. Excised tissue from adult thalli did not differentiate into archeospores, but rather regenerated directly into blades and rhizoids of foliose thalli. Tissues from young thalli of two dioecious Porphyra species (P. dentata and P pseudolinearis) also regenerated into blades and rhizoids after manipulation of the culture conditions. In addition, 1–2 celled tissue pieces of both monoecious and dioecious species were also seen to develop directly into blades. Polarity of regeneration of blades and rhizoids was observed in these species. These results suggest that ‘seed’ can be obtained through tissue culture instead of using conventional conchocelis culture for commercial nori aquaculture in Japan.
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References
Aguirre-Lipperhide M, Estrada-Rodriguez FJ, Evans LV (1995) Facts, problems, and needs in seaweed tissue culture: an appraisal. J. Phycol. 31: 677–688.
Kim NG, Notoya, M (1997) Tissue culture of Porphyra suborbiculata and P. pseudolinearis (Bangiales, Rhodophyta). Abstracts of 4th International Marine Biotechnology Conference, 218 pp.
Kirihara S, Fujikawa Y, Notoya M (1997) Axenic tissue culture of Sargassum confusum C. Agardh (Phaeophyta) as a source of seeds for artificial marine forests. J. mar. Biotechnol. 5: 142–146.
Liu XW, Gordon ME (1987) Tissue and cell culture of New Zealand Pterocladia and Porphyra species. Hydrobiologia 151/152: 147–154.
Liu XW, Kloareg B (1991) Tissue culture of Porphyra umbilicalis (Bangiales, Rhodophyta). I. The effects of plant hormones on callus induction from tissue explants. Compt. r. Acad. Sci. Paris, Ser. 3: 517–522.
Matsuo M, Notoya M, Aruga Y (1994) Life history of Porphyra suborbiculata Kjellman (Bangiales, Rhodophyta) in culture. La Mer 32: 57–63. (in Japanese)
McLachlan J (1973) Growth media — marine. In Stein JR (ed.), Handbook of Phycological Methods. Cambridge Univ. Press, New York: 25–51.
Notoya M (1995) Concrete examples of tissue culture on Porphyra spp. and perspective for its application. Kaiyo Monthly 27: 639–642. (in Japanese)
Notoya M (1996) Tissue culture of Porphyra spp. and its application for cultivation. Abstracts of 2nd Japan-Korea Aquaculture Symposium: 25–26.
Notoya M (1997a) Chloroplast changes and differentiation of callus cells in Eckloniopsis radicosa (Kjellman) Okamura (Pheophyta, Lamianariales). J. appl. Phycol. 9: 175–178.
Notoya M (1997b) A new technique of seed production on Porphyra spp. by tissue culture. Abstracts of 4th International Marine Biotechnology Conference: 211.
Notoya M, Hara M, Kim NG, Yasui H (1996a) Field and culture studies on blade growth and morphology of Porphyra okamurae Ueda (Bangiales, Rhodophyta). Abstracts of First Asia-Pacific Phycological Forum: 51.
Notoya M, Kim HG (1996b) Excised pieces of the foliose thal-lus on Porphyra suborbiculata and P pseudolinearis (Bangiales, Rhodophyta) in culture. Jap. J. Phycol. 44: 72. (in Japanese)
Notoya M, Kim NG, Suda M (1996c) Culture studies of Porphyra tenera from Matsukawaura, Fukushima Prefecture. Jap. J. Phycol. 44: 75. (in Japanese)
Perrone G, Felicini GP (1972) Sur le bourgeons adventifs de Petro-glossum nicaeese (Duby) Schotter (Rhodophycées, Gigartinales) en culture. Phycologia 11: 87–95.
Polne-Fuller M, Biniaminov M, Gibor A (1984) Vegetative propagation of Porphyra perforata. Hydrobiologia 116/117: 308–313.
Polne-Fuller M, Gibor A (1987) Calluses and callus-like growth in seaweeds: Induction and culture. Hydrobiologia 151/152: 131–138.
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© 1999 Springer Science+Business Media Dordrecht
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Notoya, M. (1999). ’seed’ production of Porphyra spp. by tissue culture. In: Kain, J.M., Brown, M.T., Lahaye, M. (eds) Sixteenth International Seaweed Symposium. Developments in Hydrobiology, vol 137. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4449-0_76
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DOI: https://doi.org/10.1007/978-94-011-4449-0_76
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