Abstract
The methods currently used for examining the relative contribution of microscopic stages to the persistence of natural populations of marine macroalgae can be inappropriate for use in subtidal habitats. Also, because of their microscopic size, direct examination and obtaining an estimate of recruitment, growth and mortality of these stages in the field is difficult. A method of removing microscopic algal stages from natural rock surfaces using watertight tents and water-soluble chemicals is presented. Also discussed is the use of a previously described method of fluorescent labelling these microscopic stages that, when examined under UV light, allows for their precise identification and growth to be determined. Together, these methods can be effective in examimng the ecology of algal microscopic stages in the field.
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References
Baselski, V. S. & M. K. Robinson, 1989. A staining kit for detection of opportunistic pathogens in bronchoalveolar lavage specimens. Amer. Clin. Lab. July, 1989: 36–37.
Blanchette, C. A., 1996. Seasonal patterns of disturbance influence the recruitment of the sea palm, Postelsia palmaeformis. J. exp. mar. Biol. Ecol. 197: 1–14.
Chapman, A. R. O., 1986. Population and community ecology of seaweeds. Adv. mar. Biol. 23: 1–161.
Cohen, J., 1988. Statistical Power Analysis for the Behavioral Sciences 2nd edn. Lawrence Erlbaum Associates, New Jersey, 567 pp.
Cole, K., 1964. Induced fluorescence in gametophytes of some Laminariales. Can. J. Bot. 42: 1173–1183.
Dayton, P. K., 1985. Ecology of kelp communities. Ann. Rev. Ecol. Syst. 16: 215–245.
Edwards, M. S., 1996. Factors regulating the recruitment of the annual alga Desmarestia ligulata along the central California coast. M.S. Thesis. San Francisco State University, 67 pp.
Flavier, A. B. & R. G. Zingmark, 1993. Macroalgal recruitment in a high marsh creek of North Inlet Estuary, South Carolina. J. Phycol. 29: 2–8.
Harlin, M. M. & J. M. Lindbergh, 1977. Selection of substrata by seaweeds: optimal surface relief. Mar. Biol. 40: 33–40.
Hoffman, A. J., 1987. The arrival of propagules at the shore: a review. Bot. mar. 30: 151–165.
Hoffman, A. J. & B. Santelices, 1991. Banks of microscopic forms: hypotheses on their functioning and comparisons with seed banks. Mar. Ecol. Prog. Ser. 79: 185–194.
Hsiao, S. I. C. & L. D. Druehl, 1973. Environmental control of gametogenesis in Laminaria saccharina. IV. In situ development of gametophytes and young sporophytes. J. Phycol. 9: 160–164.
Kain, J. M., 1975. Algal colonization of some cleared subtidal areas. J. Ecol. 63: 739–765.
Klinger, T., 1984. Allocation of the blade surface area to meiospore production in annual and perennial representatives of the genus Laminaria. M.S. Thesis, Univ. British. Columbia, Vancouver, 96 pp.
Nakazawa, S., K. Takamura & M. Abe, 1969. Rhizoid differentiation in Fucus eggs labelled with Calcofluor White and birefringence of cell wall. Bot. Mag. Tokyo 82: 41–44.
Littler, M. M. & D. S. Littler, 1985. Ecological field methods: macroalgae. Handbook of Phycological Methods. Cambridge University Press, New York, 617 pp.
Reed, D. C. & M. S. Foster, 1984. The effect of canopy shading on algal recruitment and growth in a giant kelp forest. Ecology, 65: 937–948.
Reed, D. C., T. W. Anderson, A. W. Ebeling & M. Anghera, 1997. The role of reproductive synchrony in the colonization potential of kelp. Ecology, 78: 2443–2457.
Rice, W. R., 1990. A consensus combined p-value test and the family-wide significance of component tests. Biometrics 46: 303–308.
Santelices, B., 1990. Patterns of reproduction, dispersal and recruitment in seaweeds. Oceanogr. Mar. Biol. ann. Rev. 28: 177–276.
Santelices, B., A. J. Hoffman, D. Aedo, M. Bobadilla & R. OtaÃza, 1995. A bank of microscopic forms on disturbed boulders and stones in tide pools. Mar. Ecol. Prog. Ser. 129: 215–228.
Schiel, D. R. & M. S. Foster, 1986. The structure of subtidal algal stands in temperate waters. Oceanogr. mar. Biol. Ann. Rev. 24: 265–307.
Serrão, E. A., L. Kautsky & S. H. Brawley, 1996. Distributional success of the marine seaweed Fucus vesiculosus L. in the brackish Baltic Sea correlates with osmotic capabilities of Baltic gametes. Oecologia 107: 1–12.
Wilson, O. T., 1925. Some experimental observations of marine algal successions. Ecology 6: 303–311.
Vadas, R. L., S. Johnson & T. A. Norton, 1992. Recruitment and mortality of early post-settlement stages of benthic algae. Br. phycol. J. 27:331–351.
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Edwards, M.S. (1999). Using in situ substratum sterilization and fluorescence microscopy in studies of microscopic stages of marine macroalgae. In: Kain, J.M., Brown, M.T., Lahaye, M. (eds) Sixteenth International Seaweed Symposium. Developments in Hydrobiology, vol 137. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4449-0_29
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DOI: https://doi.org/10.1007/978-94-011-4449-0_29
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