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Pea Globulins

  • Chapter
Seed Proteins

Abstract

On the basis of Osborne’s solubility classification (see Chapter 1), pea (Pisum sativum and its wild relative P. fulvum) seeds contain two major classes of protein — water-soluble albumins and salt-soluble globulins. The albumins are heterogeneous, consisting of a large number of relatively minor proteins; the majority of pea seed protein is globulin, or storage protein, but it has been suggested on the basis of their concentration that some albumins may have a storage function (Murray, 1979).

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References

  • Badenoch-Jones, J., Spencer, D., Higgins, T.J.V. and Millerd, A. (1981) The role of glycosylation in storage-protein synthesis in developing pea seeds. Planta, 153, 201–209.

    Article  CAS  Google Scholar 

  • Bäumlein, H., Wobus, U., Pustell, J. and Kafatos, F.C. (1986) The legumin gene family: structure of a B type gene of Vicia faba and a possible legumin gene specific regulatory element. Nucleic Acids Research, 14, 2707–2720.

    Article  PubMed  Google Scholar 

  • Beach, L.R., Spencer, D., Randall, P.J. and Higgins, T.J.V. (1985) Transcriptional and post-transcriptional regulation of storage protein gene expression in sulfur-deficient pea seeds. Nucleic Acids Research, 13, 999–1013.

    Article  PubMed  CAS  Google Scholar 

  • Beachy, R.N., Chen, Z.L., Horsch, R.B., Rogers, S.G., Hoffman, N.J. and Fraley, R.T. (1985) Accumulation and assembly of soybean β-conglycinin in seeds of transformed petunia plants. EMBO Journal 4, 3047–3053.

    PubMed  CAS  Google Scholar 

  • Bhattacharyya, M.K., Smith, A.M., Ellis, T.H.N., Hedley, C.L. and Martin, C. (1990) The wrinkled-seed character of pea described by Mendel is caused by a transposon-like insertion in a gene encoding starch-branching enzyme. Cell, 60, 115–122.

    Article  PubMed  CAS  Google Scholar 

  • Boulter, D. and Croy, R.R.D. (1997) The structure and biosynthesis of legume seed storage proteins: a biological solution to the storage of nitrogen in seeds. Advances in Botanical Research, 27, 1–84.

    Article  CAS  Google Scholar 

  • Boulter, D., Evans, I.M., Ellis, J.R., Shirsat, A., Gatehouse, J.A. and Croy, R.R.D. (1987) Differential gene expression in the development of Pisum sativum. Plant Physiology and Biochemistry, 25, 283–289.

    CAS  Google Scholar 

  • Bown, D., Ellis, T.H.N. and Gatehouse, J.A. (1988) The sequence of a gene encoding convicilin from pea (Pisum sativum L.) shows that convicilin differs from vicilin by an insertion near the N-terminus. Biochemical Journal, 251, 717–726.

    PubMed  CAS  Google Scholar 

  • Bown, D., Levasseur, M., Croy, R.R.D., Boulter, D. and Gatehouse, J.A. (1985) Sequence of a pseudogene in the legumin gene family of pea (Pisum sativum L.). Nucleic Acids Research, 13, 4527–4538.

    Article  PubMed  CAS  Google Scholar 

  • Casey, R. (1979a) Immunoaffinity chromatography as a means of purifying legumin from Pisum (pea) seeds. Biochemical Journal, 177, 509–520.

    PubMed  CAS  Google Scholar 

  • Casey, R. (1979b) Genetic variability in the structure of the α-subunits of legumin from Pisum — a two-dimensional gel electrophoresis study. Heredity, 43, 265–272.

    Article  CAS  Google Scholar 

  • Casey, R. and Sanger, E. (1980) Purification and some properties of a 7S seed storage protein from Pisum (pea). Biochemical Society Transactions, 8, 658.

    PubMed  CAS  Google Scholar 

  • Casey, R. and Short, M.N. (1981) Variation in amino acid composition of legumin from Pisum. Phytochemistry, 20, 21–23.

    Article  CAS  Google Scholar 

  • Casey, R., March, J.F. and Sanger, E. (1981a) N-terminal amino acid sequence of β-subunits of legumin from Pisum sativum. Phytochemistry; 20, 161–163.

    Article  CAS  Google Scholar 

  • Casey, R., March, J.F., Sharman, J.E. and Short, M.N. (1981b) The purification, N-terminal amino acid sequence and some other properties of an αM-subunit of legumin from the pea (Pisum sativum L.). Biochimica et Biophysica Acta, 670, 428–432.

    Article  CAS  Google Scholar 

  • Casey, R., Sharman, J.E., Wright, D.J., Bacon, J.R. and Guldager, P. (1982) Quantitative variability in Pisum seed globulins: its assessment and significance. Qualitas Plantarum Plant Foods for Human Nutrition, 31, 333–346.

    Article  CAS  Google Scholar 

  • Casey, R., Domoney, C. and Stanley, J. (1984) Convicilin mRNA from pea (Pisum sativum L.) has sequence homology with other legume 7S storage protein mRNA species. Biochemical Journal, 224, 661–666.

    PubMed  CAS  Google Scholar 

  • Casey, R. and Domoney, C. (1985) Variation in pea-seed storage proteins. In: Hebblethwaite, P.D., Dawkins, T.C.K. and Heath, M.C. (eds). The Pea Crop. Butterworths, London, pp. 359–367.

    Google Scholar 

  • Casey, R., Domoney, C. and Ellis, N. (1986) Legume storage proteins and their genes. Oxford Surveys of Plant Molecular and Cell Biology, 3, 1–95.

    CAS  Google Scholar 

  • Casey, R., Domoney, C. and Smith, A.M. (1993) Bichemistry and molecular biology of seed products. In: Casey, R. and Davies, D.R. (eds) Peas: Genetics, molecular biology and biotechnology, pp. 121-163, CAB.

    Google Scholar 

  • Casey, R., Domoney, C., Forster, C, Hedley, C, Hitchin, E. and Wang, T. (1998) The effect of modifying carbohydrate metabolism on seed protein gene expression in peas. Journal of Plant Physiology, 152, 636–640.

    Article  CAS  Google Scholar 

  • Chambers, S.J., Bacon, J.R. and Lambert, N. (1992) The quantitative analysis of seed proteins from peas using high performance liquid chromatography. Phytochemical Analysis, 3, 49–54.

    Article  CAS  Google Scholar 

  • Chandler, P.M., Higgins, T.J.V., Randall, P.J. and Spencer, D. (1983) Regulation of legumin levels in developing pea seeds under conditions of sulfur deficiency. Plant Physiology, 71, 47–54.

    Article  PubMed  CAS  Google Scholar 

  • Chandler, P.M., Spencer, D., Randall, P.J. and Higgins, T.J.V. (1984) Influence of sulfur nutrition on developmental patterns of some major pea seed proteins and their mRNAs. Plant Physiology, 75, 651–657

    Article  PubMed  CAS  Google Scholar 

  • Chrispeels, M.J., Higgins, T.J.V., Craig, S. and Spencer, D. (1982a) The role of the endoplasmic reticulum in the synthesis of reserve proteins and the kinetics of their transport to protein bodies in developing pea cotyledons. Journal of Cell Biology, 93, 5–14.

    Article  PubMed  CAS  Google Scholar 

  • Chrispeels, M.J., Higgins, T.J.V. and Spencer, D. (1982b) Assembly of storage protein oligomers in the endoplasmic reticulum and processing of the polypeptides in the protein bodies of developing pea cotyledons. Journal of Cell Biology, 93, 306–313.

    Article  PubMed  CAS  Google Scholar 

  • Corke, F.M.K., Hedley, C.L. and Wang, T.L. (1990) An analysis of seed development in Pisum sativum L. XL Cellular development and the deposition of storage protein in immature embryos grown in vivo and in vitro. Protoplasma, 155, 127–135.

    Article  Google Scholar 

  • Croy, R.R.D., Derbyshire, E., Krishna, T.G. and Boulter, D. (1979) Legumin of Pisum sativum and Vicia faba. New Phytologist, 83, 29–35.

    Article  CAS  Google Scholar 

  • Croy, R.R.D., Gatehouse, J.A., Evans, I.M. and Boulter, D. (1980a) Charaterisation of the storage protein subunits synthesised in vitro by polyribosomes and RNA from developing pea (Pisum sativum L.) I. Legumin. Planta, 148, 49–56.

    Article  CAS  Google Scholar 

  • Croy, R.R.D., Gatehouse, J.A., Evans, I.M. and Boulter, D. (1980b) Characterisation of the storage protein subunits synthesised in vitro by polyribosomes and RNA from developing pea (Pisum sativum L.) II. Vicilin. Planta, 148, 57–63.

    Article  CAS  Google Scholar 

  • Croy, R.R.D., Gatehouse, J.A., Tyler, M. and Boulter, D. (1980c) The purification and characterization of a third storage protein (convicilin) from the seeds of pea (Pisum sativum L.). Biochemical Journal, 191, 509–516.

    PubMed  CAS  Google Scholar 

  • Croy, R.R.D., Lycett, G.W., Gatehouse, J.A., Yarwood, J.N. and Boulter, D. (1982) Cloning and analysis of cDNAs encoding plant storage protein precursors. Nature, 295, 76–79.

    Article  CAS  Google Scholar 

  • Croy, R.R.D. and Gatehouse, J.A. (1985) Genetic engineering of seed proteins: current and potential applications. In: Dodds, J.H. (ed), Plant Genetic Engineering, Cambridge University Press, Cambridge, pp. 143–268.

    Google Scholar 

  • Croy, R.R.D., Evans, I.M., Yarwood, J.N., Harris, N., Gatehouse, A., Shirsat, A.H., Kang, A., Ellis, J.R., Thompson, A. and Boulter, D. (1988) Expression of pea legumin sequences in pea, Nicotiana and yeast. Biochemie und Physiologie der Pflanzen, 183, 183–197.

    CAS  Google Scholar 

  • Danielsson, C.E. (1949) Seed globulins of the gramineae and leguminosae. Biochemical Journal, 44, 387–400.

    PubMed  CAS  Google Scholar 

  • Davies, D.R. (1980) The ra locus and legumin synthesis in Pisum sativum. Biochemical Genetics, 18, 1207–1219.

    Article  PubMed  CAS  Google Scholar 

  • Derbyshire, E., Wright, D.J. and Boulter, D. (1976) Legumin and vicilin, storage proteins of legume seeds. Phytochemistry, 15, 3–24.

    Article  CAS  Google Scholar 

  • Dickinson, C.D., Evans, R.P. and Nielsen, N.C. (1988) RY repeats are conserved in the 5?-flanking regions of legume seed-protein genes. Nucleic Acids Research, 16, 371.

    Article  PubMed  CAS  Google Scholar 

  • Domoney, C. and Casey, R. (1984) Storage protein precursor polypeptides in cotyledons of Pisum sativum L. Identification of, and isolation of a cDNA clone for, an 80 000-Mr legumin-related polypeptide. European Journal of Biochemistry, 139, 321–327.

    Article  PubMed  CAS  Google Scholar 

  • Domoney, C. and Casey, R. (1985) Measurement of gene number for seed storage proteins in Pisum. Nucleic Acids Research, 13, 687–699.

    Article  PubMed  CAS  Google Scholar 

  • Domoney, C., Barker, D. and Casey, R. (1986a) The complete deduced amino acid sequences of legumin ß-polypeptides from different genetic loci in Pisum. Plant Molecular Biology, 7, 467–474.

    Article  CAS  Google Scholar 

  • Domoney, C, Ellis, T.H.N and Davies, D.R. (1986b) Organization and mapping of legumin genes in Pisum. Molecular and General Genetics, 202, 280–285.

    Article  CAS  Google Scholar 

  • Domoney, C. and Casey, R. (1987) Changes in legumin messenger RNAs throughout seed development in Pisum sativum L. Planta, 170, 562–566.

    Article  CAS  Google Scholar 

  • Domoney, C. and Casey, R. (1990) Another class of vicilin gene in Pisum. Planta, 183, 39–42.

    Google Scholar 

  • Doyle, J.J., Schuler, M.A., Godette, W.D., Zenger, V., Beachy, R.N. and Slightom, J.L. (1986) The glycosylated seed storage proteins of Glycine max and Phaseolus vulgaris: structural homologies of genes and proteins. Journal of Biological Chemistry, 261, 9228–9238.

    PubMed  CAS  Google Scholar 

  • Ellis, T.H.N., Domoney, C, Castleton, J., Cleary, W. and Davies, D.R. (1986) Vicilin genes of Pisum. Molecular and General Genetics, 205, 164–169.

    Article  CAS  Google Scholar 

  • Ellis, J.R., Shirsat, A.H., Hepher A., Yarwood, J.N., Gatehouse, J.A., Croy, R.R.D. and Boulter, D. (1988) Tissue-specific expression of a pea legumin gene in seeds of Nicotiana plumbaginifolia. Plant Molecular Biology, 10, 203–214.

    Article  CAS  Google Scholar 

  • Ellis, T.H.N., Turner, L., Hellens, R.P., Lee, D., Harker, C.L., Enard, C, Domoney, C. and Davies, D.R. (1992). Linkage maps in pea. Genetics, 130, 649–663.

    PubMed  CAS  Google Scholar 

  • Ersland, D.R., Brown, J.W.S., Casey, R. and Hall, T.C. (1983) The storage proteins of Phaseolus vulgaris L., Vicia faba L. and Pisum sativum L. In: Gottschalk, W. and Muller, H.P. (eds) Seed Proteins. Biochemistry, Genetics, Nutritive Value. Martinus Nijhoff/Dr. W. Junk, The Hague, pp. 355–375.

    Google Scholar 

  • Evans, I.M., Gatehouse, J.A., Croy, R.R.D. and Boulter, D. (1984) Regulation of the transcription of storage-protein mRNA in nuclei isolated from developing pea (Pisum sativum L.) cotyledons. Planta, 160, 559–568.

    Article  CAS  Google Scholar 

  • Gatehouse, J.A., Croy, R.R.D. and Boulter, D. (1980) Isoelectric-focusing properties and carbohydrate content of pea (Pisum sativum) legumin. Biochemical Journal, 185, 497–503.

    PubMed  CAS  Google Scholar 

  • Gatehouse, J.A., Croy, R.R.D., Morton, H., Tyler, M. and Boulter, D. (1981) Characterisation and subunit structures of the vicilin storage proteins of pea (Pisum sativum L.). European Journal of Biochemistry, 118, 627–633.

    Article  PubMed  CAS  Google Scholar 

  • Gatehouse, J.A., Evans, I.M., Bown, D., Croy, R.R.D. and Boulter, D. (1982a) Control of storage-protein synthesis during seed development in pea (Pisum sativum L). Biochemical Journal, 208, 119–127

    PubMed  CAS  Google Scholar 

  • Gatehouse, J.A., Lycett, G.W., Croy, R.R.D. and Boulter, D. (1982b) The post-translational proteolysis of the subunits of vicilin from pea (Pisum sativum L.). Biochemical Journal, 207, 629–632.

    PubMed  CAS  Google Scholar 

  • Gatehouse, J.A., Lycett, G.W., Delauney, A.J., Croy, R.R.D. and Boulter, D. (1983) Sequence specificity of the post-translational proteolytic cleavage of vicilin, a seed storage protein of pea(Pisum sativum L.). Biochemical Journal, 212, 427–432.

    PubMed  CAS  Google Scholar 

  • Gatehouse, J.A., Evans, I.M., Croy, R.R.D. and Boulter, D. (1986) Differential expression of genes during legume seed development. Philosophical Transactions of the Royal Society of London B, 314, 367–384.

    Article  CAS  Google Scholar 

  • Gatehouse, J.A., Bown, D., Gilroy, J., Levasseur, M., Castleton, J. and Ellis, T.H.N. (1988) Two genes encoding ‘minor’ legumin polypeptides in pea (Pisum sativum L.). Biochemical Journal, 250, 15–24.

    PubMed  CAS  Google Scholar 

  • Gueguen, J. (1983) Legume seed protein extraction, processing and end-product characteristics. Qualitas Plantarum, Plant Foods for Human Nutrition, 32, 267–303.

    Article  CAS  Google Scholar 

  • Guldager, P. (1978) Immunoelectrophoretic analysis of seed proteins from Pisum sativum L. Theoretical and Applied Genetics, 53, 241–250.

    Google Scholar 

  • Hauxwell, A.J., Corke, F.M.K., Hedley, C.L. and Wang, T.L. (1990) Storage protein gene expression is localised to regions lacking mitotic activity in developing pea embryos. An analysis of seed development in Pisum sativum XIV. Development, 110, 283–289.

    PubMed  CAS  Google Scholar 

  • Heim, U., Baumlein, H. and Wobus, U. (1994) The legumin gene family: a reconstructed Vicia faba legumin gene encoding a high-molecular-weight subunit is related to type B genes. Plant Molecular Biology, 25, 131–135.

    Article  PubMed  CAS  Google Scholar 

  • Higgins, T.J.V. (1984) Synthesis and regulation of major proteins in seeds. Annual Review of Plant Physiology, 35, 191–221.

    Article  CAS  Google Scholar 

  • Higgins, T.J.V and Spencer, D. (1981) Precursor forms of pea vicilin subunits. Plant Physiology, 67, 205–211.

    Article  PubMed  CAS  Google Scholar 

  • Higgins, T.J.V., Newbigin, E.J., Spencer, D., Llewellyn, D.J. and Craig, S. (1988) The sequence of a pea vicilin gene and its expression in transgenic tobacco plants. Plant Molecular Biology, 11, 683–695.

    Article  CAS  Google Scholar 

  • Hirano, H., Gatehouse, J.A. and Boulter, D. (1982) The complete amino acid sequence of a subunit of the vicilin seed storage protein of pea (Pisum sativum L.). FEBS Letters, 145, 99–102.

    Article  CAS  Google Scholar 

  • Howley, P.M. and Gatehouse, J.A. (1997) A 38 bp repeat sequence within the pea seed storage protein promoter of legA is a binding site for a nuclear DNA-binding protein. Plant Molecular Biology, 33, 175–180.

    Article  PubMed  CAS  Google Scholar 

  • I’Anson, K.J., Miles, M.J., Bacon, J.R., Carr H.J., Lambert, N., Morris, V.J. and Wright, D.J. (1988) Structure of the 7S globulin from pea. Internationaljournal of Biological Macromolecules, 10, 311–337.

    Article  Google Scholar 

  • Krishna, T.G., Croy, R.R.D. and Boulter, D. (1979) Heterogeneity in subunit composition of the legumin of Pisum sativum. Phytochemistry, 18, 1879–1880.

    Article  CAS  Google Scholar 

  • Lee, D., Ellis, T.H.N., Turner, L., Hellens, R.P. and Cleary, W.G. (1990) A copia-like element in Pisum demonstrates the uses of dispersed repeated sequences in genetic analysis. Plant Molecular Biology, 15, 707–722.

    Article  PubMed  CAS  Google Scholar 

  • Lycett, G.W., Delauney, A.J., Gatehouse, J.A., Gilroy, J., Croy, R.R.D. and Boulter, D. (1983) The vicilin gene family of pea (Pisum sativum L.): a complete cDNA coding sequence for preprovicilin. Nucleic Acids Research, 11, 2367–2380.

    Article  PubMed  CAS  Google Scholar 

  • Lycett, G.W., Croy, R.R.D., Shirsat, A.H. and Boulter, D. (1984a) The complete nucleotide sequence of a legumin gene from pea (Pisum sativum L.). Nucleic Acids Research, 12, 4493–5406.

    Article  PubMed  CAS  Google Scholar 

  • Lycett, G.W., Delauney, A.J., Zhao, W., Gatehouse, J.A., Croy, R.R.D. and Boulter, D. (1984b) Two cDNA clones coding for the legumin protein of Pisum sativum L. contain sequence repeats. Plant Molecular Biology, 3, 91–96.

    Article  CAS  Google Scholar 

  • Lycett, G.W., Croy, R.R.D., Shirsat, A.H., Richards, D.M. and Boulter, D. (1985) The 5’-flanking regions of three pea legumin genes: comparison of the DNA sequences. Nucleic Acids Research, 13, 6733–6743.

    Article  PubMed  CAS  Google Scholar 

  • Mahmoud, S.H. and Gatehouse, J.A. (1984) Inheritance and mapping of vicilin storage protein genes in Pisum sativum L. Heredity, 53, 185–191.

    Article  CAS  Google Scholar 

  • March, J.F., Domoney, C. and Casey, R. (1987) Legumin heterogeneity in Pisum. Biochemical Genetics, 25, 449–458.

    Article  PubMed  CAS  Google Scholar 

  • March, J.F., Pappin, D.J.C. and Casey, R. (1988) Isolation and characterization of a minor legumin and its constituent polypeptides from Pisum sativum (pea). Biochemical Journal, 250, 911–915.

    PubMed  CAS  Google Scholar 

  • Matta, N.K. and Gatehouse, J.A. (1981) Modification of the basic subunits of pea legumin on storage. Phytochemistry, 22, 2621–2623.

    Article  Google Scholar 

  • Matta, N.K., Gatehouse, J.A. and Boulter, D. (1981) Molecular and subunit heterogeneity of legumin of Pisum sativum L. (Garden Pea)—a multi-dimensional gel electrophoretic study. Journal of Experimental Botany, 32, 1295–1307.

    Article  CAS  Google Scholar 

  • Matta, N.K. and Gatehouse, J.A. (1982) Inheritance and mapping of storage protein genes in Pisum sativum L. Heredity, 48, 383–392.

    Article  CAS  Google Scholar 

  • Millerd, A., Thomson, J.A. and Schroeder, H.E. (1978) Cotyledonary storage proteins in Pisum sativum III. Patterns of accumulation during development. Australian Journal of Plant Physiology, 5, 519–534.

    Article  CAS  Google Scholar 

  • Millerd, A., Thomson, J.A. and Randall, P.J. (1979) Heterogeneity of sulphur content in the storage proteins of pea cotyledons. Planta, 146, 463–466.

    Article  CAS  Google Scholar 

  • Murray, D.R. (1979) A storage role for albumins in pea cotyledons. Plant, Cell and Environment, 2, 221–226.

    Article  Google Scholar 

  • Newbigin, E.J., deLumen, B.O., Chandler, P.M., Gould, A., Blagrove, R.J., March, J.F., Kortt, A.A. and Higgins, T.J.V. (1990) Pea convicilin: structure and primary sequence of the protein and expression of a gene in the seeds of transgenic tobacco. Planta, 180, 461–470.

    Article  CAS  Google Scholar 

  • Osborne, T.B. and Campbell, G.F. (1898) Proteids of the pea. Journal of the American Chemical Society, 20, 348–362.

    Article  Google Scholar 

  • Pang, P.P., Pruitt, R.E. and Meyerowitz, E.M. (1988) Molecular cloning, genomic organization, expression and evolution of 12S seed storage protein genes of Arabidopsis thaliana. Plant Molecular Biology, 11, 805–820.

    Article  CAS  Google Scholar 

  • Perez, M.D., Chambers, S.J., Bacon, J.R., Lambert, N., Hedley, C.L. and Wang, T.L. (1993) Seed protein content and composition of near-isogenic and induced mutant pea lines. Seed Science Research, 3, 187–194.

    Article  CAS  Google Scholar 

  • Perez-Grau, L. and Goldberg, R.B. (1989) Soybean seed protein genes are regulated spatially during embryogenesis. The Plant Cell, 1, 1095–1109.

    PubMed  CAS  Google Scholar 

  • Plietz, P., Damaschun, G., Zirwer, D., Gast, K. and Schlesier, B. (1983) Structure of 7S seed globulin from Phaseolus vulgaris L. in solution. International Journal of Biological Macromolecules, 5, 356–360.

    Article  CAS  Google Scholar 

  • Plumb, G.W., Carr, H.J., Newby, V.K. and Lambert, N. (1989) A study of the trypsinolysis of pea 11 S globulin. Biochimica et Biophysica Acta, 999, 281–288.

    Article  CAS  Google Scholar 

  • Randall, P.J., Thomson, J.A. and Schroeder, H.E. (1979) Cotyledonary storage proteins in Pisum sativum. IV. Effects of sulfur, phosphorus, potassium and magnesium deficiencies. Australian Journal of Plant Physiology, 6, 11–24.

    Article  CAS  Google Scholar 

  • Rerie, W.G., Whitecross, M.I. and Higgins, T.J.V. (1990) Nucleotide sequence of an A-type legumin gene from pea. Nucleic Acids Research, 18, 655

    Article  PubMed  CAS  Google Scholar 

  • Rerie, W.G., Whitecross, M. and Higgins, T.J.V. (1991) Developmental and environmental regulation of pea legumin genes in transgenic tobacco. Molecular and General Genetics, 225, 148–157

    PubMed  CAS  Google Scholar 

  • Rerie, W.G., Newbigin, E.J. and Higgins, T.J.V. (1992) Genes encoding seed globulins in legumes. Advances in Plant Cell Biochemistry and Biotechnology, 1, 53–104.

    CAS  Google Scholar 

  • Schroeder, H.E. (1982) Quantitative studies on the cotyledonary proteins in the genus Pisum. Journal of the Science of Food and Agriculture 33, 623–633.

    Article  PubMed  CAS  Google Scholar 

  • Schroeder, H.E. and Brown, A.H.D. (1984) Inheritance of legumin and albumin contents in a cross between round and wrinkled peas. Theoretical and Applied Genetics, 68, 101–107.

    Article  CAS  Google Scholar 

  • Schroeder, H., Gollasch, S., Tabe, L., Moore, A., Spencer, D. and Higgins, T. (1995) The expression and stability of transgenes in peas (Pisum sativum L.). Proceedings of the 2nd European Conference on Grain Legumes (AEP), Copenhagen, pp. 422-433.

    Google Scholar 

  • Sengupta-Gopalan, C, Reichert, N.A., Barker, R.F., Hall, T.C. and Kemp, J.D. (1985) Developmentally regulated expression of the bean ß-phaseolin gene in tobacco seed. Proceedings of the National Academy of Sciences, USA, 82, 3320–3324.

    Article  CAS  Google Scholar 

  • Shirsat, A.H. (1988) A transposon-like structure in the 5’ flanking sequence of a legumin gene from Pisum sativum. Molecular and General Genetics, 212, 129–133.

    Article  PubMed  CAS  Google Scholar 

  • Shirsat, A., Wilford, N., Croy, R. and Boulter, D. (1989) Sequences responsible for the tissue specific promoter activity of a pea legumin gene in tobacco. Molecular and General Genetics, 215, 326–331.

    Article  PubMed  CAS  Google Scholar 

  • Shirsat, A.H., Meakin, P.J. and Gatehouse, J.A. (1990) Sequences 5’ to the conserved 28 bp Leg box element regulate the expression of pea seed storage protein gene legA. Plant Molecular Biology, 15, 685–693.

    Article  PubMed  CAS  Google Scholar 

  • Slightom, J.L., Sun, S.M. and Hall, T.C. (1983) Complete nucleotide sequence of a French bean storage protein gene: phaseolin. Proceedings of the National Academy of Sciences, USA, 80, 1897–1901.

    Article  CAS  Google Scholar 

  • Spencer, D. (1984) The physiological role of storage proteins in seeds. Philosophical Transactions of the Royal Society of London B, 304, 275–285.

    Article  CAS  Google Scholar 

  • Spencer, D. and Higgins, T.J.V. (1980) The biosynthesis of legumin in maturing pea seeds. Biochemistry International, 1, 502–509.

    CAS  Google Scholar 

  • Spencer, D., Chandler, P.M., Higgins, T.J.V., Inglis, A.S. and Rubira, M. (1983) Sequence interrelationships of the subunits of vicilin from pea seeds. Plant Molecular Biology, 2, 259–267.

    Article  CAS  Google Scholar 

  • Spencer, D., Rerie, W.G., Randall, P.J. and Higgins, T.J.V. (1990) The regulation of pea seed storage protein genes by sulfur stress. Australian Journal of Plant Physiology, 17, 355–363.

    Article  CAS  Google Scholar 

  • Staswick, P.E., Hermodson, M.A. and Nielsen, N.C. (1984) Identification of the cystines which link the acidic and basic components of the glycinin subunits. Journal of Biological Chemistry, 259, 13431–13435.

    PubMed  CAS  Google Scholar 

  • Takaiwa, F., Kikuchi, S. and Oono, K. (1987) A rice glutelin gene family—a major type of glutelin mRNA can be divided into two classes. Molecular and General Genetics, 208, 15–22.

    Article  CAS  Google Scholar 

  • Takei, Y., Yamauchi, D. and Minamikawa, T. (1989) Nucleotide sequence of the canavalin gene from Canavalia gladiata seeds. Nucleic Acids Research, 17, 4381.

    Article  PubMed  CAS  Google Scholar 

  • Thompson, A.J., Bown, D., Yaish, S. and Gatehouse, J.A. (1991) Differential expression of seed storage protein genes in the pea legJ subfamily; sequence of gene legK. Biochemie und Physiologie der Pflanzen, 187, 1–12.

    CAS  Google Scholar 

  • Thompson, A.J., Evans, I.M., Boulter, D., Croy, R.R.D. and Gatehouse, J.A. (1989) Transcriptional and posttranscriptional regulation of seed storage-protein gene expression in pea (Pisum sativum L.). Planta, 179, 279–287.

    Article  CAS  Google Scholar 

  • Thomson, J.A. and Schroeder, H.E. (1978) Cotyledonary storage proteins in Pisum sativum. II. Hereditary variation in components of the legumin and vicilin fractions. Australian Journal of Plant Physiology, 5, 281–294.

    Article  CAS  Google Scholar 

  • Thomson, J.A., Schroeder, H.E. and Dudman, W.F. (1978) Cotyledonary storage proteins in Pisum sativum. I. Molecular heterogeneity. Australian Journal of Plant Physiology, 5, 263–279.

    Article  CAS  Google Scholar 

  • Thomson, J.A., Schroeder, H.E. and Tassie, A.M. (1980) Cotyledonary storage proteins in Pisum sativum V. Further studies on molecular heterogeneity in the vicilin series of holoproteins. Australian Journal of Plant Physiology, 7, 271–282.

    Article  CAS  Google Scholar 

  • Tierney, M.L., Bray, E.A., Allen, R.D., Ma, Y., Drong, R.F., Slightom, J.L. and Beachy, R.N., (1987) Isolation and characterization of a genomic clone encoding the ß-subunit of ß-conglycinin. Planta, 172, 356–363.

    Article  CAS  Google Scholar 

  • Tulloch, P.A. and Blagrove, R.J. (1985) Electron microscopy of seed-storage globulins. Archives of Biochemistry and Biophysics, 241, 521–532.

    Article  PubMed  CAS  Google Scholar 

  • Turner, S.R., Barratt, D.H.P. and Casey, R. (1990) The effect of different alleles at the r locus on the synthesis of seed storage proteins in Pisum sativum. Plant Molecular Biology, 14, 793–803.

    Article  PubMed  CAS  Google Scholar 

  • Turner, L., Hellens, R.P., Lee, D. and Ellis, T.H.N. (1993) Genetic aspects of the organization of legumin genes in pea. Plant Molecular Biology, 22, 101–112.

    Article  PubMed  CAS  Google Scholar 

  • Vonder Haar, R.A., Allen, R.D., Cohen, E.A., Nessler, C.L. and Thomas, T.L. (1988) Organization of the sunflower 1 1S storage protein gene family. Gene, 74, 433–443.

    Article  Google Scholar 

  • Weiland, M.E., Miles, M.J., Lambert, N., Morris, I., Coombs, J.H. and Pethica, J.B. (1989) Structure of the globular protein vicilin revealed by scanning tunnelling microscopy. International Journal of Biological Macromolecules, 11, 29–32.

    Article  Google Scholar 

  • Weschke, W., Bäumlein, H. and Wobus, U. (1987) Nucleotide sequence of a field bean (Vicia faba L. var. minor) vicilin gene. Nucleic Acids Research, 15, 10065.

    Article  PubMed  CAS  Google Scholar 

  • Wright, D.J. and Boulter, D. (1974) Purification and subunit structure of legumin of Vicia faba L. (broad bean). Biochemical Journal, 141, 413–418.

    PubMed  CAS  Google Scholar 

  • Wright, D.J. and Bumstead, M.R. (1984) Legume proteins in food technology. Philosophical Transactions of the Royal Society of London B, 304, 381–393.

    Article  CAS  Google Scholar 

  • Yang, L., Domoney, C, Casey, R. and Hall, T.C. (1990) Processing Pisum sativum seed storage protein precursors in vitro. Cell Research, 2, 153–162.

    Article  Google Scholar 

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© 1999 Springer Science+Business Media Dordrecht

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Casey, R., Domoney, C. (1999). Pea Globulins. In: Shewry, P.R., Casey, R. (eds) Seed Proteins. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4431-5_9

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  • DOI: https://doi.org/10.1007/978-94-011-4431-5_9

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