Abstract
We have started the study of plant genes encoding homologous of yeast halotolerance gene HAL3. HAL3 is a yeast protein which regulates the cell cycle and the tolerance to salt stress through inhibition of the PPZ1 type 1 protein phosphatase. Expression of the these plant genes in yeast hal3 mutants partially complements their salt sensitivity, suggesting that HAL3 function is conserved between yeast and plants. Yeast HAL3 and the plant homologous show striking homology with a group of proteins found in fungi and animals. Preliminary analysis of purified recombinant proteins from Escherichia coli suggest that these proteins belongs to a new eukaryotic family of protein presumably involved in regulation of the cell cycle and ion homeostasis.
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Literature Cited
Boyer, J.S. (1982) Productivity and environment. Science, 218, 443–448
Bray EA (1997) Plant responses to water deficit. Trends Plant Sci 2: 48–54
Clotet J, Gari E, Aldea M, Arino J (1999) The yeast ser/thr phosphatases sit4 and ppzl play opposite roles in regulation of the cell cycle. Mol Cell Biol 3: 2408–15
Dichtl B, Stevens A, Tollervey D (1997) Lithium toxicity in yeast is due to inhibition of RNA processing enzymes. EMBO J 16: 7184–7195
Di Como CJ, Bose R, Arndt T (1995) Overexpression of SIS2 which contains an extremely acidic region, increases expression of SWI4, CLN1 and CLN2 in sit4 mutans. Genetics 139: 95–107
Ferrando A, Kron SJ, Rios G, Fink GR, Serrano R (1995) Regulation of cation transport in Saccharomyces cerevisiae by the salt tolerance gene HAL3. Mol. Cell. Biol 15: 5470–5481
Flower TJ, Hajibaghery MA, Clipson NJW (1986) Halophytes. Quarterly Review of Biology 61: 313–317
Gaxiola RA, Rao R, Sherman A, Grisafi P, Alper SL, Fink GR (1999) The Arabidopsis thaliana proton transporters, AtNhxl and Avpl, can function in cation detoxification in yeast. Proc Natl Acad Sci USA 96: 1480–1485
Gil-Mascarell R, Lopez-Coronado JM, Belles JM, Serrano R, Rodriguez PL (1999) The Arabidopsis HAL2-like gene family includes a novel sodium-sensitive phosphatase. Plant J 17: 373–83
Glaser H-U, Thomas D, Gaxiola R, Montrichard F, Surdin-Kerjan Y, Serrano R (1993) Salt tolerance and methionine biosynthesis in Saccharomyces cerevisiae involve a putative phosphatase gene. EMBO J 12: 3105–3110
Holmberg N, Billow, L (1998) Improving stress tolerance in plants by gene transfer. Trends Plant Sci 3: 61–66
Ingram J, Bartels, D (1996) The molecular basis of dehydration tolerance in plants. Annu Rev Plant Physiol Plant Mol Biol 47: 377–403
Johnston LH, Eberly SL, Chapman JW, Araki H, Sugino A (1990) The product of the Saccharomyces cerevisiae cell cycle gene DBF2 has homology with protein kinases and is periodically expressed in the cell cycle. Mol Cell Biol 10: 1358–66
Lee JH, Van Montagu M, Verbruggen N (1999) A highly conserved kinase is an essential component for stress tolerance in yeast and plant cells. Proc Natl Acad Sci USA 96: 5873–7
Murguía JM, Bellés JM, Serrano R (1995) A salt sensitive 3’(2’), 5’-bisphosphate nucleotidase involved in sulfate activation. Science 267: 232–234
Murguía JM, Bellés JM, Serrano R (1996) The yeast HAL2 nucleotidase is an in vivo target of salt toxicity. J Biol Chem 271: 29029–29033
Nadal E, Clotet J, Posas F, Serrano R, Gómez N, Ariño J (1998) Regulation of salt tolerance by HAL3p is mediated through the PPZ1 Ser/Thr protein phosphatase. Proc Natl Aced Sci USA 95: 7357–7362
Pardo JM, Reddy MP, Yang S, Maggio A, Huh GH, Matsumoto T, Coca MA, PainoD’Urzo M, Koiwa H, Yun DJ, Watad AA, Bressan RA, Hasegawa PM (1998) Stress signaling through Ca2+/calmodulin-dependent protein phosphatase calcineurin mediates salt adaptation in plants. Proc Natl Aced Sci USA 95: 9681–6
Quintero FJ, Garciadeblas B, Rodrígez-Navarro A (1996) The SAL1 gene of Arabidopsis, encoding an enzyme with 3’(2’), 5’-Bisphosphate nucleotidase and inositol polyphosphate 1-phosphatase activities, increases salt tolerance in yeast. Plant Cell 8: 529–537
Serrano R (1994) Yeast halotolerance genes: crucial ion transport and metabolic reactions in salt tolerance. In Biochemical and cellular mechanisms of stress tolerance in plants (Cherry, J.H., ed.). NATO ASI Series, Vol. H 86 Springer-Verlag Berlin Heidelberg, pp 371–380
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Espinosa-Ruiz, A. et al. (2000). Plant homologues to the yeast halotolerance gene HAL3. In: Cherry, J.H., Locy, R.D., Rychter, A. (eds) Plant Tolerance to Abiotic Stresses in Agriculture: Role of Genetic Engineering. NATO Science Series, vol 83. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4323-3_9
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DOI: https://doi.org/10.1007/978-94-011-4323-3_9
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