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CLSM analysis of serotonin-immunoreactive neurons in the central nervous system of Nais variabilis, Slavina appendiculata and Stylaria lacustris (Oligochaeta: Naididae)

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Aquatic Oligochaetes

Part of the book series: Developments in Hydrobiology ((DIHY,volume 139))

Abstract

The organization of the nervous system and the distribution of serotoninergic neurons were analysed in three species of Naididae using immunohistochemical labelling methods in conjunction with confocal laser-scanning microscopy. In the posterior region of the ventral nerve cord the serotoninergic neurons are arranged in an alternating pattern in successive ganglia, clearly different from the pattern in the anterior neuromeres. This unique arrangement of 5HT-neurons is presumably an autapomorphic character of the Naididae or at least of a subtaxon including these three species. In all three species the cerebral ganglion is located in segments behind the prostomium, as has previously been found in terrestrial oligochaetes, where the relocation of the brain from the initially anterior position in the prostomium into the following segments can be explained as a functional adaptation to a life spent burrowing through comparatively firm substrates. This structural correspondence is regarded as an indication that the Naididae, being typical limnic oligochaetes, are derived from a terrestrial form.

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References

  • Anctil, M., J.-P. De Waele & A. K. Pani, 1990. Monoamines in the nervous system of the tube-worm Chaetopterus variopedatus (Polychaeta): Biochemical detection and serotonin immunoreactivity. Cell. Tiss. Res. 259: 81–92.

    Article  CAS  Google Scholar 

  • Anlauf, A., 1989. Die Charakterisierung von Populationen des Schlammröhrenwurms Tubifex tubifex (Müller) mit Hilfe von enzymelektrophoretischen, populationsgenetischen und ökologischen Methoden. Doctorate thesis, Universität Köln: 127 pp.

    Google Scholar 

  • Barlow, L. A. & J. W. Truman, 1991. Patterns of serotonin and SCP immunoreactivity during metamorphosis of the nervous system of the red abalone, Haliotis rufescens. J. Neurobiol. 23: 829–844.

    Article  Google Scholar 

  • Bullock, T. H. & G. A. Horridge, 1965. Structure and function in the nervous systems of invertebrates. Vol. 1. W. H. Freeman and Company, San Francisco. 798 pp.

    Google Scholar 

  • Clark, R. B., 1964. Dynamics in metazoan evolution. The origin of the coelom and segments. Clarendon Press, Oxford: 313 pp.

    Google Scholar 

  • Côté, S. L., A. Ribeiro-Da-Silva & A. C. Cuello, 1993. Current protocols for light microscopy immunocytochemistry. In A. C. Cuello (ed.), Immunohistochemistry. 2nd Ed. John Wiley & Sons Ltd, Chichester: 147–168.

    Google Scholar 

  • Csoknya, M., I. Lengvari, L. Hiripi, K. Elekes, J. Vincze, M. Szelier & J. Hamori, 1993. Serotonin content during the regeneration of nervous system in earthworm Lumbricus terrestris L., Oligochaeta). Acta Biol. Szeg. 39: 39–49.

    CAS  Google Scholar 

  • Denk, H., 1989. Immunohistochemische Färbemethoden. In P. Böck (ed.), Romeis Mikroskopische Technik. 17th Edn. Urban und Schwarzberg, München: 252–261.

    Google Scholar 

  • Ehinger, B. & H. E. Myhrberg, 1971. Neuronal localization of dopamine, noradrenaline and 5-HT in the central and peripheral nervous system of Lumbricus terrestris (L.). Histochemistry 28: 265–275.

    Article  PubMed  CAS  Google Scholar 

  • Elofsson, R. & M. Carlberg, 1989. Gland cells in the tentacles of the jellyfish Cyanea lamarcki reactive with an antibody against 5-hydroxytryptamine. Cell. Tiss. Res. 255: 419–422.

    Google Scholar 

  • Erséus, C., 1990. Cladistic analysis of the subfamilies within the Tubificidae (Oligochaeta). Zool. Scr. 19: 57–63.

    Article  Google Scholar 

  • Evans, B. D. & R. L. Calabrese, 1989. Small cardioactive peptidlike immunoreactivity and its colocalization with FMRFamidelike immunoreactivity in the central nervous system of the leech Hirudo medicinalis. Cell. Tiss. Res. 257: 187–199.

    CAS  Google Scholar 

  • Fine, A., 1993. Immunocytochemical applications of confocal microscopy. In A. C. Cuello (ed.), Immunohistochemistry. 2nd Edn. John Wiley & Sons Ltd, Chichester: 394–407.

    Google Scholar 

  • Hanström, B., 1928. Vergleichende Anatomie des Nervensystems der Wirbellosen Tiere. Springer Verlag, Berlin. 628 pp.

    Google Scholar 

  • Hartmann-Schröder, G., 1993. annelida. In H.-E. Gruner (ed.), Lehrbuch der Speziellen Zoologie, Band I: Wirbellose Tiere-3. Teil: Mollusca, Sipunculida, Echiurida, Annelida, Onychophora, Tardigrada, Pentastomida. Gustav Fischer Verlag, Stuttgart. 276–469.

    Google Scholar 

  • Hess, W. N., 1925. Nervous system of the earthworm, Lumbricus terrestris. J. Morph. 40: 235–259.

    Article  Google Scholar 

  • Hessling, R. & W. Westheide, 1999. CLSM analysis of development and structure of the central nervous system of Enchytraeus crypticus (“Oligochaeta”, Enchytraeidae). Zoomorphology 119: 37–47.

    Article  Google Scholar 

  • Isossimov, V. V., 1926. Zur Anatomie des Nervensystems der Lumbriculiden. Zool. Jb. Anat. 48: 365–404.

    Google Scholar 

  • Lacalli, T. C., 1984. Structure and organization of the nervous system in the trochophore larva of Spirobranchus. Phil. Trans. r. Soc. London 306: 79–135.

    Article  Google Scholar 

  • Learner, M. A., G. Lochhead & B. D. Hughes, 1978. A review of the biology of British Naididae (Oligochaeta) with emphasis on the lotic environment. Freshw. Biol. 8: 357–375.

    Article  Google Scholar 

  • Lent, C. M. & M. H. Dickinson, 1984. Serotonin integrates the feeding behavior of the medicinal leech. J. comp. Physiol. 154: 457–471.

    Article  CAS  Google Scholar 

  • Marsden, C. A. & G. A. Kerkut, 1969. Fluorescence microscopy of the 5-HT and catecholamine containing cells in the central nervous system of the leech Hirudo medicinalis. Comp. Biochem. Physiol. 31: 851–862.

    Article  PubMed  CAS  Google Scholar 

  • Omodeo, P., 1998. History of Clitellata. Ital. J. Zool. 65: 51–73.

    Article  Google Scholar 

  • Purschke, G., 1999. Terrestrial polychaetes models for the evolution of the Clitellata (Annelida)? Hydrobiologia 406: 87–99.

    Article  Google Scholar 

  • Purschke, G., W. Westheide, D. Rohde & R. O. Brinkhurst, 1993. Morphological reinvestigation and phylogenetic relationship of Acanthobdella peledina (Annelida, Clitellata). Zoomorphology 113:91–101.

    Article  Google Scholar 

  • Sawyer, R. T., 1986. Leech biology and behavior I. Anatomy, physiology and behavior. Clarendon Press, Oxford: 1–418.

    Google Scholar 

  • Sperber, C., 1948. A taxonomical study of the Naididae. Zool. Bidr. Upps. 28: 1–292.

    Google Scholar 

  • Spörhase-Eichmann, U., H. Gras & F.-W. Schürmann, 1987a. Patterns of serotonin-immunoreactive neurons in the central nervous system of the earthworm Lumbricus terrestris L.-I. Ganglia of the ventral nerve cord. Cell. Tiss. Res. 249: 601–614.

    Article  Google Scholar 

  • Spörhase-Eichmann, U., H. Gras & F.-W. Schürmann, 1987b. Patterns of serotonin-immunoreactive neurons in the central nervous system of the earthworm Lumbricus terrestris L.-II. Rostral and caudal ganglia. Cell. Tiss. Res. 249: 625–632.

    Article  Google Scholar 

  • Steinbusch, H. W. M. & R. Nieuwenhuys, 1981. Localization of serotonin-like immunoreactivity in the central nervous system and pituitary of the rat, with special references to the innervation of the hypothalamus. Adv. Exp. Med. Biol. 133: 7–35.

    Article  PubMed  CAS  Google Scholar 

  • Steinbusch, H. W. M., A. A. J. Verhofstad & H. W. J. Joosten, 1978. Localisation of serotonin in the central nervous system by immunohistochemistry: a description of a specific and sensitive technique and some applications. Neuroscience 3: 811–819.

    Article  PubMed  CAS  Google Scholar 

  • Stent, G. S., W. B. Kristan, S. A. Torrence, K. A. Freach & D. A. Weisblat, 1992. Development of the leech nervous system. Int. Rev. Neurobiol. 33: 109–193.

    Article  PubMed  CAS  Google Scholar 

  • Stephenson, J., 1930. the Oligochaeta. Clarendon Press, Oxford: 978 pp.

    Google Scholar 

  • Stuart, D. K., S. S. Blair & D. A. Weisblat, 1987. Cell lineage, cell death, and the developmental origin of identified serotonin-and dopamine-containing neurons in the leech. J. Neurosci. 7: 1107–1122.

    PubMed  CAS  Google Scholar 

  • Timm, T., 19811. On the origin and evolution of aquatic Oligochaeta. Eesti NSV Teaduste Akadeemia Toimetised 30: 174–181.

    Google Scholar 

  • Wachs, B., 1967. Die Oligochaeten-Fauna der Fließgewaesser unter besonderer Berü cksichtigung der Beziehungen zwischen Tubificiden-Besiedlung und dem Substrat. Arch. Hydrobiol. 63: 310–386.

    Google Scholar 

  • Wallace, B. G., 1981. Neurotransmitter chemistry. In K. J. Muller, J. G. Nicholls & G. S. Stent (eds.), Neurobiology of the leech. Cold Spring Harbor Laboratory, Cold Spring Harbor: 147–172.

    Google Scholar 

  • Westheide, W., 1997. The direction of evolution within the Polychaeta. J.Nat. Hist. 31: 1–15.

    Article  Google Scholar 

  • Windoffer, R., 1992. Immunohistochemische und elektronenmikroskopische Untersuchungen am Nervensystem zweier Dinophilus-Arten mit unterschiedlichem Lebenszyklus. Doctorate thesis, Universität Osnabrück. 187 pp.

    Google Scholar 

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Authors and Affiliations

  1. Spezielle Zoologie, Universität Osnabrück FB Biologie/Chemie, Barbarastr. 11, D-49069, Osnabrück, Germany

    René Hessling, Monika C. Müller & Wilfried Westheide

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  1. René Hessling
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  2. Monika C. Müller
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  3. Wilfried Westheide
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Editors and Affiliations

  1. Zoological Department, University College, Belfield, Stillorgan Road, Dublin 4, Ireland

    B. M. Healy

  2. National Water Research Institute, 867 Lakeshore Rd., Burlington, Ontario, L7R 4A6, Canada

    T. B. Reynoldson

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© 1999 Springer Science+Business Media Dordrecht

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Hessling, R., Müller, M.C., Westheide, W. (1999). CLSM analysis of serotonin-immunoreactive neurons in the central nervous system of Nais variabilis, Slavina appendiculata and Stylaria lacustris (Oligochaeta: Naididae). In: Healy, B.M., Reynoldson, T.B., Coates, K.A. (eds) Aquatic Oligochaetes. Developments in Hydrobiology, vol 139. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-4207-6_22

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  • DOI: https://doi.org/10.1007/978-94-011-4207-6_22

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5829-2

  • Online ISBN: 978-94-011-4207-6

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