Metabolite Channelling and Protein—Protein Interactions in the Urea Synthesis Pathway

  • Natalie S. Cohen
  • Chia-Wei Cheung
  • Luisa Raijman
Part of the NATO Science Series book series (ASHT, volume 74)


The pathway of urea synthesis of mammalian liver has proved to be an excellent model for the investigation of the intracellular organization of soluble enzymes. Studies of the behaviour and regulation of the enzymes of this pathway in situ (reviewed by Cohen et al., 1997) have conclusively shown that the latter operates as a tightly organized system, referred to by Srere (1987), as a metabolon. Intermediates are retained within the pathway and are channelled between sequential enzymes at every step, even across the mitochondrial membranes. By channelled we mean that the intermediates do not mix freely throughout the bulk aqueous phase of the cell.


Carbamoyl Phosphate Urea Synthesis Intact Mitochondrion Ornithine Transcarbamylase Carbamyl Phosphate 
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Copyright information

© Springer Science+Business Media Dordrecht 2000

Authors and Affiliations

  • Natalie S. Cohen
    • 1
  • Chia-Wei Cheung
    • 2
  • Luisa Raijman
    • 3
  1. 1.Department of Molecular Pharmacology and Toxicology, School of PharmacyUniversity of Southern CaliforniaCaliforniaUSA
  2. 2.Department of Molecular BiologyCity of Hope Beckman Research InstituteCaliforniaUSA
  3. 3.Lafayette HillPennsylvaniaUSA

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