Summary
For the first time, somatic embryos have been obtained in Quercus canariensis. Immature embryos were isolated from acorns collected during July 1994, and cultured in an established solid medium. Initiation of the embryogenic process was studied monitoring the inductive effect of 2,4 dichlorophenoxyacetic acid (2,4-D) and the effect of organic N (amino acid). Development of somatic embryos was observed 3 weeks after initiation of the culture both in the presence or in the absence of auxin and glutamine. Although the frequency of embryogenic induction varied among the different treatments, the main factor influencing somatic embryo formation was the state of development of the initial expiants, those collected at earlier stages being the most embryogénie.
Ploidy level and DNA content of somatic embryos were analyzed by flow cytometry. Embryos were purely diploid after one year in culture, in contrast to observations in other species.
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© 1996 Springer Science+Business Media Dordrecht
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Bueno, M.A., Gómez, A., Vicente, O., Manzanera, J.A. (1996). Stability in Ploidy Level During Somatic Embryogenesis in Quercus Canariensis . In: Ahuja, M.R., Boerjan, W., Neale, D.B. (eds) Somatic Cell Genetics and Molecular Genetics of Trees. Forestry Sciences, vol 49. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3983-0_4
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DOI: https://doi.org/10.1007/978-94-011-3983-0_4
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