Abstract
Random Amplified Polymorphic DNA (RAPDs) were used for genetic mapping and QTL detection in an F2 selfed progeny of maritime pine. The seedlings were raised for two years in a greenhouse under accelerated growth conditions. Total height was measured at different maturation stages and corresponding QTLs were located in different genomic regions, providing an explanation for the absence of juvenile-mature correlation for height. Within each shoot cycle, QTL for traits related to the initiation of foliar primordia in the bud and the elongation of the stem were mapped in different chromosomes, suggesting that the activity of the apical and subapical meristems was controlled by separate genetic mechanisms. Rapid mapping rely on the use of arbitrary primers and PCR-based technique to amplify polymorphic DNA fragments. These markers are anonymous and may be physiologically irrelevant if one want to understand the biological meaning of the detected QTLs. As part of our mapping programme, we are currently developping a map based on protein markers reveal by 2-D PAGE. Such translated regions of the genome might be very useful for the characterization of QTLs.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bahrman, N. and Petit, R. (1995) Genetic polymorphism in maritime pine (Pinus pinaster Ait.) assessed by two-dimensional gel electrophoresis of needle, bud and pollen proteins. J. Mol. Evol. 41, 231–237.
Beavis, W.D., Grant, D., Albertsen, M, and Fincher, R. (1991) Quantitative trait loci for plant height in four maize populations and their associations with qualitative genetic loci. Theor. Appl. Genet. 83, 141–145.
Causse, M., Rocher, J.P., Henry, A.M., Charcosset, A., Prioul, J.L., and de Vienne, D. (1995) Genetic dissection of the relationship between carbon metabolism and early growth in maize, with emphasis on key-enzyme loci. Molecular Breeding 3, 259–272.
Dietz, E.J. (1983) Permutation tests for association between two distance matrices. Syst. Zool. 32, 21–26.
Goldman, I.L., Rocheford, T.R., and Dudley, J.W. (1994) Quantitative trait loci influencing protein and starch concentration in the Illinois long term selection maize strains. Theor. Appl. Genet. 87, 217–224.
Grattapaglia, D., and Sederoff, R. (1994) Genetic linkage maps of Eucalyptus grandis and E. urophylla using a pseudo-testcross mapping strategy and RAPD markers. Genetics 137, 1121–1137.
Greenwood, M., Hopper, CA., and Hutchison, K.W. (1989) Maturation in larch. I. Effect of age on shoot growth, foliar characteristics and DNA methylation. Plant Physiol. 90, 406–412.
Greenwood, M.S., and Volkaert, H.A. (1992) Morphophysiological traits as markers for the early selection of conifer genetic families. Can. J. For. Res. 22, 1001–1008.
Hackett, W.P. (1985) Juvenility, maturation and rejuvenation in woody plants. Hort. Reviews 7, 109–155.
Hackett, W. P., and Murray, J. R. (1993) Micropropagation of woody plants, in: M.R. Ahuja (ed.), Maturation and Rejuvenation in Woody Species, Kluwer Academic Publishers, Dordrecht, Netherlands, pp. 93–105.
Haffner, V., Enjarlic, F., Lardet, L., and Carron, M.P. (1991) Maturation of woody plants: a review of metabolic and genomic aspects. Ann. Sci. For. 48, 615–630.
Hutchison, K.W., Sherman, CD., Weber, J., Smith, S.S., Singer, P.B., and Greenwood, M.S. (1990) Maturation in larch. II. Effect of age on photosynthesis and gene expression in developping foliage. Plant Physiol. 94, 1308–1315.
Hutchison, K.W., and M.S. Greenwood, (1991) Molecular approaches to gene expression during conifer development and maturation. For. Ecol. Manage. 43, 273–286.
Kremer, A. (1984) Distribution of relative growth rates and variation of cytohistological zonation in apical meristems of seedlings of two contrasting open — pollinated jack pine (Pinus banksiana) families. Can. J. For. Res. 14, 297–310.
Kremer, A., Lascoux, M., and N’Guyen, A. (1991) Morphogenetic subdivision of height growth and early selection in maritime pine, in: Proc. 21st Southern Forest Tree Improvement Conference. Knoxville, Tennessee, pp. 203–221.
Lander, E.S., Green, P., Abrahamson, J., Barlow, A., Daly, M.J., Lincoln, S.E., and Newburg, L. (1987) Mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1, 174–181.
Lander, E.S., and Botstein, D. (1989) Mapping Mendelian factors underlying quantitative traits using RFLP linkage maps. Genetics 121, 185–199.
Lanner RM (1976) Pattern of shoot development in Pinus and their relationship to growth potential, in M.G.R. Cannell and F.T. Last (eds.), Tree Physiology and Yield Improvement, Academic press, pp. 223–289.
Lascoux, D.M., Dormling, I., and Kremer, A. (1992) Germination and phenology of 1-year-old maritime pine (Pinus pinaster Ait.) seedlings under continuous light. Trees 7, 48–58.
Lascoux, D.M., Kremer, A., and Dormling, I. (1993a) Growth and phenology of 1-year-old maritime pine (Pinus pinaster) seedlings under continuous light: implication for early selection. Can. J. For. Res. 23, 1325–1336.
Lascoux, D.M., Novitol Paino, E., Sierra de Grado, R., Kremer A., and Dormling, I. (1993b) Maturation of maritime pine (Pinus pinaster Ait.) seedling after exposure to a period of continuous light. Tree Physiol. 12, 363–378.
Lascoux, M., Kang, H., and Lundkvist, K. (1994) Growth of 24 full-sib families of Pinus sylvestris L. at six relative nutrient addition rates. Scand. J. For. Res. 9, 115–123.
Plomion, C., O’Malley, D.M., and Durel, C-E. (1995a) Genomic analysis in maritime pine (Pinuspinaster). Comparison of two RAPD maps using selfed and open-pollinated seeds of the same individual. Theor. Appl. Genet. 90, 1028–1034.
Plomion, C., Bahrman, N., Durel, C-E., and O’Malley, D.M. (1995b) Genomic mapping in Pinus pinaster (maritime pine) using RAPD and protein markers. Heredity 74, 661–668.
Poethig, R.S. (1990) Phase change and the regulation of shoot morphogenesis in plants. Science 250, 923–930.
Romberger, J.A., and Gregory, R.A. (1977) The shoot apical ontogeny of the Picea abies seedling. III. Some age-related aspects of morphogenesis. Am. J. Bot. 64, 622–630.
Stabel P, Eriksson T, Engström P (1990) Changes in proteins synthesis upon cytokinin — mediated adventitious bud induction and during seedling development in norway spruce, Picea Abies. Plant Physiol. 92, 1174–1183.
Tanksley, S.D. (1993) Mapping polygenes. Ann. Rev. Genet 27, 205–233.
Touzet, P., Winkler R.G., and Helentjaris T. (1995) Combined genetic and physiological analysis of a locus contributing to quantitative variation. Theor. Appl. Genet. 91, 200–205.
Williams, J.G.K., Kubelik, A.R., Livak, K.J., and Rafalski, J.A. (1990) DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nuc. Acids. Res. 18, 6531–6535.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1996 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Plomion, C., Bahrman, N., Durel, CE., O’Malley, D.M., Kremer, A. (1996). Genetic Dissection of Phenotypic Traits in Maritime Pine Using Rapd and Protein Markers. In: Ahuja, M.R., Boerjan, W., Neale, D.B. (eds) Somatic Cell Genetics and Molecular Genetics of Trees. Forestry Sciences, vol 49. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3983-0_31
Download citation
DOI: https://doi.org/10.1007/978-94-011-3983-0_31
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5761-5
Online ISBN: 978-94-011-3983-0
eBook Packages: Springer Book Archive