Abstract
Signal recognition particle (SRP) is a cytoplasmic ribonucleoprotein that facilitates the co-translational insertion of proteins into the endoplasmic reticulum in eukaryotes and the cytoplasmic membrane in prokaryotes. In prokaryotes, the SRP-RNA binds a single 54 kD polypeptide subunit, while in eukaryotes, five additional subunits are bound. A homologue of the 54 kD subunit of SRP was found in chloroplasts; it is 44% and 27% identical to SRP54 from E. coli and dog, respectively (1). This paper describes our progress in determining the structure of cpSRP and its role in the chloroplast. In particular we find that cpSRP, comprised of 43 and 54 kD subunits, plays a role in the biogenesis of light harvesting chlorophyll proteins (LHCP), the major proteins of the thylakoid membrane. LHCP form a large family of related proteins that have three to four transmembrane domains. They are synthesized in the cytoplasm, and are targeted to the thylakoid membrane through the stroma by a post-translational mechanism. Evidence is also presented that cpSRP54 functions independently of cpSRP43 in the co-translational targeting of chloroplast encoded proteins.
Preview
Unable to display preview. Download preview PDF.
References
Franklin, A. E., and Hoffman, N. E. (1993) J. Biol. Chem. 268, 22175–22180
Schuenemann, D., Gupta, S., Persello-Cartieaux, F., Klimyuk, V. I., Jones, J., D.G., Nussaume, L., and Hoffman, N. E. (1998) Proc. Nat. Acad. Sci. (in Press)
Cline, K., and Henry, R. (1996) Annu. Rev. Cell Cev. Biol. 12, 1–26
Klimyuk, V. I., Persello-Cartieaux, F., Havaux, M., Contard, P., Meiherhoff, K., Schuenemann, D., Gouet, P., Hoffman, N. E., Jones, J., D.G., and Nussaume, L. (submitted)
Cline, K. (1986) J. Biol. Chem. 261, 14804–14810
Hoffman, N. E., and Franklin, A. E. (1994) Plant Physiol. 105, 295–304
Payan, L. A., and Cline, K. (1991) J Cell Biol. 112, 603–613
Li, X. X., Henry, R., Yuan, J. G., Cline, K., and Hoffman, N. E. (1995) Proc. Nat. Acad. Sci 92, 3789–3793
Fulson, D., and Cline, K. (1988) Plant Physiol 88, 1146–1153
Packer, J. C. L., and Howe, C. J. (1998) Mol. Microbiol. 27, 508–510
Ribes, V., Romisch, K., Giner, A., Dobberstein, B., and Tollervey, D. (1990) Cell 63 (3), 591–600
Pilgrim, M. L., Wijk, K.-J. v., Parry, D. H., Sy, D. A. C., and Hoffman, N. E. (1998) Plant J 13, 177–186
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Hoffman, N.E. et al. (1998). Structure of chloroplast signal recognition particle and its role in chloroplast biogenesis. In: Garab, G. (eds) Photosynthesis: Mechanisms and Effects. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3953-3_720
Download citation
DOI: https://doi.org/10.1007/978-94-011-3953-3_720
Publisher Name: Springer, Dordrecht
Print ISBN: 978-0-7923-5547-2
Online ISBN: 978-94-011-3953-3
eBook Packages: Springer Book Archive