Skip to main content
SpringerLink
Log in
Book cover

Epithelia pp 99–115Cite as

Cultured Epithelial Cells Derived from Human Fetal Pancreatic Duct

  • Chapter

  • 62 Accesses

Abstract

The human pancreatic duct is a complex system that consists of a branching network of channels between the acini of the pancreas and the duodenum. Adjacent to acini are centroacinar cells which form the termini of intralobular ducts. The smallest branches of these join to form larger intralobular ducts which in turn pass out of the pancreatic lobules to form interlobular ducts. Interlobular ducts join to form ducts of increasing diameter which eventually lead into the main pancreatic duct. The pancreatic duct not only provides a channel for the passage of digestive enzymes from the acini to the duodenum, but also actively secretes bicarbonate-rich fluid and mucins1,2.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Case, R. M. and Argent, B.E. (1986). Bicarbonate secretion by pancreatic duct cells: mechanism and control. In Go, V. L. W., Gardner, J. D., Brooks, F. P., Lebenthal, E., Di Magno, E. P., Scheele, G. A. (eds.) The Exocrine Pancreas: Biology, Pathobiology and Diseases, pp. 213–244 (New York: Raven Press)

    Google Scholar 

  2. Forstner, G. and Forstner, J. (1986). Mucus: biosynthesis and secretion. In Go, V. L. W., Gardner, J. D., Brooks, F. P., Lebenthal, E., Di Magno, E. P., Scheele, G. A. (eds.) The Exocrine Pancrease: Biology, Pathobiology and Diseases, pp. 283–286. (New York: Raven Press)

    Google Scholar 

  3. Cubilla, A. L. and Fitzgerald, P. J. (1975). Morphological patterns of primary non-endocrine human pancreas carcinoma. Cancer Res., 35 2234–2248

    PubMed  CAS  Google Scholar 

  4. Cubilla, A. L. and Fitzgerald, P. J. (1978). Pancreas cancer. 1. Duct adenocarcinoma. Pathol. Annu., 13, 241–289

    PubMed  Google Scholar 

  5. Stoner, G. D., Harris, C. C., Bostwick, D. G., Jones, R. T., Trump, B. F., Kingsbury, E. W., Fineman, E. and Newkirk, C. (1978). Isolation and characterization of epithelial cells from bovine pancreatic duct. In Vitro, 14, 581–590

    Article  PubMed  CAS  Google Scholar 

  6. Hirata, K., Oku, T. and Freeman, A. E. (1982). Duct, exocrine, and endocrine components of cultured fetal mouse pancreas. In Vitro, 18, 789–799

    Article  PubMed  CAS  Google Scholar 

  7. Resau, J. H., Hudson, E. A. and Jones, R. T. (1983). Organ expiant culture of adult Syrian golden hamster pancreas. In Vitro, 19, 315–325

    Article  PubMed  CAS  Google Scholar 

  8. Sato, T., Mamoru, S., Hudson, E. A. and Jones, T. (1983). Characterization of bovine pancreatic ductal cells isolated by a perfusion-digestion technique. In Vitro, 19, 651–660

    Article  PubMed  CAS  Google Scholar 

  9. Jones, R. T., Hudson, E. A. and Resau, J. H. (1981). A review of in vitro and in vivo culture research for the study of pancreatic carcinogenesis. Cancer, 47, 1490–1496

    Article  PubMed  CAS  Google Scholar 

  10. Laitio, M., Lev, R. and Orlic, D. (1974). The developing human fetal pancreas: an ultrastructural and histochemical study with special reference to exocrine cells. J. Anat., 117, 619–634

    PubMed  CAS  Google Scholar 

  11. Adda, G., Hannoun, L. and Loygue, J. (1984). Development of the human pancreas: variations and pathology. A tentative classification. Anat. Clin., 5, 275–283

    Article  PubMed  CAS  Google Scholar 

  12. Brock, D. J. H., Bedgood, D. and Hayward, C. (1984). Prenatal diagnosis of cystic fibrosis by assay of amniotic fluid microvillar enzymes. Hum. Genet., 65, 248–251

    Article  PubMed  CAS  Google Scholar 

  13. Boué, A., Muller, F., Nezelof, C, Oury, J. F., Duchatel, F., Dumez, Y., Aubry, M. C. and Boué, J. (1986). Prenatal diagnosis in 200 pregnancies with a 1-in-4 risk of cystic fibrosis. Hum. Genet., 74, 288–297

    Article  PubMed  Google Scholar 

  14. Ornoy, A., Arnon, J., Katznelson, D., Granat, M., Caspi, B. and Chemke, J. (1987). Pathological confirmation of cystic fibrosis in the fetus following prenatal diagnosis. Am. J. Med. Genet., 28, 935–947

    Article  PubMed  CAS  Google Scholar 

  15. Harris, A. and Coleman, L. (1987). Establishment of a tissue culture system for epithelial cells derived from human pancreas: a model for the study of cystic fibrosis. J. Cell Sci., 87, 695–703

    PubMed  Google Scholar 

  16. Harris, A. and Coleman, L. (1988). Cultured epithelial cells derived from human fetal pancreas as a model for the study of cystic fibrosis: further analysis on the origins and nature of the cell types. J. Cell Sci., 90, 73–77

    PubMed  Google Scholar 

  17. Lane, E. (1982). Monoclonal antibodies provide specific intramolecular markers for the study of epithelial tonofilament organization. J. Cell Biol., 92, 665–673

    Article  PubMed  CAS  Google Scholar 

  18. Bramwell, M. E., Bhavanandan, V. P., Wiseman, G. and Harris, H. (1983). Structure and function of the Ca antigen. Br. J. Cancer, 48, 177

    Article  PubMed  CAS  Google Scholar 

  19. Burchell, J., Durbin, H. and Taylor-Papadimitriou, J. (1983). Complexity of expression of antigenic determinants recognised by monoclonal antibodies HMFG1 and HMFG2, in normal and malignant human mammary epithelial cells. J. Immunol., 131, 508–513

    PubMed  CAS  Google Scholar 

  20. Borowitz, M. J., Tuck, F. L., Sindelar, W. F., Fernsten, P. D. and Metzgar, R. S. (1984). Monoclonal antibodies against human pancreatic adenocarcinoma: distribution of DU-PAN-2 antigen on glandular epithelia and adenocarcinomas. J. Natl. Cancer Inst., 72, 999–1003

    PubMed  CAS  Google Scholar 

  21. Lan, M. S., Finn, O. J., Fernsten, P. D. and Metzgar, R. S. (1985). Isolation and properties of a human pancreatic adenocarcinoma-associated antigen, DU-PAN-2. Cancer Res., 45, 305–310

    PubMed  CAS  Google Scholar 

  22. Magnani, J. L., Nilsson, B., Brockhaus, M., Zopf, D., Steplewski, Z., Koprowski, H. and Ginsburg, V. (1982). A monoclonal antibody-defined antigen associated with gastrointestinal cancer is a ganglioside containing sialated lacto-N-fucopentaose II. J. Biol. Chem., 257, 14635–14639

    Google Scholar 

  23. Harris, H. (1987). The Ca antigen: structure, function and clinical application. In Daar, A. S. (ed.) Tumour Markers in Clinical Practice, pp. 115–128 (Oxford: Blackwell Scientific Publications).

    Google Scholar 

  24. Graham, F. L. and Van der Eb, A. J. (1973). A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology, 52, 456–467

    Article  PubMed  CAS  Google Scholar 

  25. Fromm, M. and Berg, P. (1982). Deletion mapping of DNA regions required for SV40 early region promoter function in vivo. J. Mol. Appl. Genet., 1, 437–481

    Google Scholar 

  26. Gray, M. A., Harris, A., Coleman, L., Greenwell, J. R. and Argent, B. E. (1989). Two types of chloride channel on duct cells cultured from human fetal pancreas. Am. J. Physiol., 257, C240–251

    PubMed  CAS  Google Scholar 

  27. Chirgwin, J. M., Przbyla, A. E., MacDonald, R. J. and Rutter, W. J. (1979). Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry, 18, 5294–5299

    Article  PubMed  CAS  Google Scholar 

  28. Rommens, J. M. et al (1989). Identification of the cystic fibrosis gene: chromosome walking and jumping. Science, 245, 1059–1065

    Article  PubMed  CAS  Google Scholar 

  29. Riorden, J. R. et al. (1989). Identification of the cystic fibrosis gene: cloning and characterization of complementary DNA. Science, 245, 1066–1073

    Article  Google Scholar 

  30. Kerem, B. et al. (1989). Identification of the cystic fibrosis gene: genetic analysis. Science, 245, 1073–1080

    Article  PubMed  CAS  Google Scholar 

Download references

Authors
  1. A. Harris
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Division of Biomedical Sciences, University of California, Riverside, CA, USA

    Christopher J. Jones

Rights and permissions

Reprints and permissions

Copyright information

© 1990 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

Harris, A. (1990). Cultured Epithelial Cells Derived from Human Fetal Pancreatic Duct. In: Jones, C.J. (eds) Epithelia. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3905-2_7

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-3905-2_7

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5739-4

  • Online ISBN: 978-94-011-3905-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation