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Electrophysiological and Morphological Studies on Secretory and Reabsorptive Segments of the Human Eccrine Sweat Gland and on Primary Cell Cultures Established from these Regions

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Epithelia

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Abstract

Epithelial tissues are routinely dissected from laboratory animals for use in scientific research but investigations on human epithelia have been hampered by the difficulty in obtaining fresh normal samples routinely. Skin represents an obvious exception in view of its accessibility and has provided cells for fibroblast culture (for example references 1 and 2) and glands for direct study (for example references 3–6), organ maintenance7, primary cell culture8–14 and established cell lines15,16. The glands can either be microdissected from small skin plugs obtained by punch biopsy, or isolated by the shearing procedure from larger skin specimens obtained from individuals undergoing elective surgery. In the first report on shearing4, the skin specimens were pretreated with collagenase, but this was later found to be unnecessary3,5,7,17. The isolation of skin glands without the need to preincubate with collagenase was especially advantageous, particularly if the glands were to be used for direct physiological and biochemical investigations where enzyme pretreatment might prove deleterious. Further information on the bulk isolation of skin glands can be found in Chapter 13 by Terence Kealey.

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  1. Division of Biomedical Sciences, University of California, Riverside, CA, USA

    Christopher J. Jones

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Jones, C.J. (1990). Electrophysiological and Morphological Studies on Secretory and Reabsorptive Segments of the Human Eccrine Sweat Gland and on Primary Cell Cultures Established from these Regions. In: Jones, C.J. (eds) Epithelia. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3905-2_19

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  • DOI: https://doi.org/10.1007/978-94-011-3905-2_19

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