Abstract
An in vitro bone forming system is described in which interface reactions were studied with calcium phospates. A layer of hydroxyapatite or fluorapatite was applied on tissue culture plastic using the plasma-spray technique. Interactions between the bone forming cells and the extracellular matrix on one hand and the plasma-sprayed calcium phosphates on the other hand, were studied using light microscopy (LM), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and X-ray microanalysis (XRMA).
Collagen fibrils were seen attached parallel to the plasma-sprayed calcium phosphate surface, after which needle-shaped crystals were deposited onto the materials from 4 weeks on. Frequently an electron-dense, lamina limitans-like zone was present between the newly formed bone and the calcium phosphates. The presence of this layer points to an active contribution in normal bone metabolism. Both hydroxyapatite and fluorapatite seemed to behave as nucleation sites for crystal precipitation.
The results of this paper show that by using this in vitro bone forming system interface reactions with biomaterials can be studied.
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De Bruijn, J.D., Klein, C.P.A.T., Terpstra, R.A., De Groot, K., Van Blitterswijk, C.A. (1991). Study of the Bone-Biomaterial Interface Reactions in an in Vitro Bone Forming System: A Preliminary Report. In: Williams, K.R., Toni, A., Middleton, J., Pallotti, G. (eds) Interfaces in Medicine and Mechanics—2. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3852-9_45
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DOI: https://doi.org/10.1007/978-94-011-3852-9_45
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