Abstract
One of the prime candidates for somatic cell gene therapy is severe combined immunodeficiency (SCID) caused by a defect in the gene coding for adenosine deaminase (ADA). The development of gene therapy protocols for this disorder would greatly benefit from the availability of powerful methods for the detection and selection of cells expressing newly indroduced ADA vectors. Here we describe an expression vector that could help to meet such demands. A fusion gene was constructed which carries the sequences encoding human ADA fused in frame to the coding region of the bacterial beta-galactosidase gene (lacZ). We showed that this ADA-lacZ fusion gene encodes a hybrid protein in which the enzymatic activities known to be associated with ADA and beta-galactosidase are both retained. Cells expressing this gene could therefore be detected and selected using the sensitive and versatile methods that exist for cells producing the lacZ gene product.
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Shen, Q., van Beusechem, V.W., Einerhand, M.P.W., Valerio, D. (1990). A Novel Approach for the Selection and Detection of Cells Transfected with Adenosine Deaminase Expression Vectors. In: Crommelin, D.J.A., Schellekens, H. (eds) From Clone to Clinic. Developments in Biotherapy, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3780-5_45
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DOI: https://doi.org/10.1007/978-94-011-3780-5_45
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