Abstract
Antigen carriers become increasingly important as components in subunit vaccines. Three carrier systems that are potential candidates to be used as antigen carriers in vaccines are: liposomes, immune-stimulating complexes (iscoms), and AlPO4-detergent complexes. In this paper some of our experiences with these systems are reported, using pore protein I from Neisseria gonorrhoeae as a model antigen. Immunisation experiments were performed to obtain information about the immunogenicity of the pore protein (PIC3) in the three presentation forms (all in the presence of AlPO4). The influence of the lipid composition of uni-lamellar vesicles as well as the influence of the antigen density (for liposomes and for iscoms) on the immunogenicity was investigated. Also, a dose-response curve of iscoms and AlPO4-detergent-complexes was obtained.
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References
Edelman, R. (1980) “Vaccine adjuvants”, Rev. Infect. Dis. 2, 370–383
Warren, H.S., Vogel, F.R. and Chedid, L.A. (1986) “Current status of immunological adjuvants”, Ann. Rev. Immunol. 4, 369–388.
Gregoriadis, G. and Allison, A. (1980) Liposomes in biological systems, Wiley and Sons, Chichester, UK.
Desiderio, J. and Cambell, S.G. (1985) “Immunization against experimental murine salmonellosis with liposome-associated O-antigen”, Infect. Immun. 48, 658–663.
El Guink, N.E., Kris, R.M., Goodman-Snitkoff, G., Small Jr., P.A. and Manniono, R.J. (1989) “Intranasal immunization with proteo-liposomes protects against influenza”, Vaccine 7, 147–151.
Rhalem, A., Bourdien, C., Luffan, G. and Pery P. (1988) “Vaccination of mice with liposome-entrapped adult antigens of Nippostrongylus brasiliensis”, Ann. Inst. Pasteur/Immunol. 139, 157–166.
Kahl, L.P., Scott, C.A., Lebchuk, R., Gregoriadis, G. and Liew, F.Y. (1989) “Vaccination against murine cutaneous leishmaniasis by using Leishmania major antigen/liposomes. Optimization and assessment of the requirement for intravenous immunization”, J. Immunol. 142, 4441–4449.
Morein, B., Sundquist, B, Höglund, S., Dalsgaard, K. and Osterhaus, A. (1984) “Iscom, a novel structure for antigenic presentation of membrane proteins from enveloped virus”, Nature 308, 457–460.
Höglund, S., Dalsgaard, K., Lövgren, K., Sundquist, B., Osterhaus, A. and Morein, B. (1989) “ISCOMs and immunostimulation with viral antigens”, Subcellular Biochemistry 15, 39–68.
De Vries, P., van Binnendijk, R., van der Marel, P., van Wezel, A.L., Voorma, H.O., Sundquist, B., UytdeHaag, F.G.C.M. and Osterhaus, A.D.M.E. (1988) “Measles virus fusion protein presented in an immune-stimulating complex (iscom) induces haemolysisinhibiting and fusion-inhibiting antibodies, virus specific T-cells and protection in mice”, J. Gen. Virol. 69, 549–559.
Varsanyi, T.M., Morein, B., Löve, A. and Norrby, E. (1987) “Protection against lethal measles virus infection in mice by immune-stimulating complexes containing the hemagglutinin or fusion protein”, J. Virol. 61: 3896–3901.
Morgan, A.J., Finerty, S., Lövgren, K., Scullion, F.T. and Morein, B. (1988) “Prevention of Epstein-Barr (EB) virus-induced lymphoma in Cottontop Tamarins by vaccination with the EB virus envelope glycoprotein gp 340 incorporated into immune-stimulating complexes”, J. Gen. Virol. 69, 2093–2096.
Osterhaus, A., Weijer, K., UytdeHaag, F., Jarret, O., Sundquist, B. and Morein, B. (1985) “Induction of protective immune response in cats by vaccination with Feline Leukemia virus iscom”, J. Immunol. 135, 591–596.
De Vries, P., UytdeHaag, F.G.C.M. and Osterhaus, A.D.M.E. (1988) “Canine Distemper Virus (CDV) immune-stimulating complexes (iscoms), but not measles virus iscoms, protect dogs against CDV infection”, J. Gen. Virol. 69, 2071–2083.
Osterhaus, A.D.M.E., UytdeHaag, F.G.C.M., Visser, I.K.G., Vedder, E.J., Reijnder, P.J.H., Kuiper, J. and Brugge, H.N. (1989) “Seal vaccination success”, Nature 337, 21.
Trudel, M., Boulay, G., Séguin, C., Nadon, F. and Lussier, G. (1988) “Control of infectious bovine rhinotracheitis in calves with a BHV-1 subunit-ISCOM vaccine”, Vaccine 6, 525–529.
Teerlink, T., Beuvery, E.C., Evenberg, D. and van Wezel, A.L. (1987) “Synergistic effect of detergents and aluminium phosphate on the humoral immune response to bacterial and viral membrane proteins”, Vaccine 5, 307–314.
Teerlink, T., Breas, R., van Eijk, R., Tiesjema, R. and Beuvery, E.C. (1985) “Isolation and immunological characterization of gonococcal porin protein”, in G. Schoolnik et al. (eds.), American Society for Microbiology, Washington DC, 259–264.
Van Dalen, F., Kersten, G., Teerlink, T., Beuvery, E.C. and Crommelin, D.J.A. (1988) “Preparation and characterization of liposomes with incorporated Neissería gonorrhoeae protein IB and amphiphilic adjuvants”, J. Contr. Release 7, 123–132.
Kersten, G.F.A., van de Put, A.-M., Teerlink, T., Beuvery, E.C. and Crommelin, D.J.A. (1988) “Immunogenicity of liposomes and iscoms containing the major outer membrane protein of Neissería gonorrhoeae: influence of protein content and liposomal bilayer composition”, Infect. Immun. 56, 1661–1664.
Kersten, G.F.A., Teerlink, T., Derks, H.J.G.M., Verkleij, A.J., van Wezel, A.L., Crommelin, D.J.A. and Beuvery, E.C. (1988) “Incorporation of the major outer membrane protein of Neissería gonorrhoeae in saponin-lipid complexes (iscoms): chemical analysis, some structural features, and comparison of their immunogenicity with three other antigen delivery systems”, Infect. Immun. 56, 432–438.
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Kersten, G.F.A., Beuvery, E.C., Crommelin, D.J.A. (1990). Antigen Carriers: A Success Determining Factor for Subunit Vaccines?. In: Crommelin, D.J.A., Schellekens, H. (eds) From Clone to Clinic. Developments in Biotherapy, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3780-5_39
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DOI: https://doi.org/10.1007/978-94-011-3780-5_39
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