Abstract
The possibility to use monoclonal antibodies (Moabs) for in vivo radioimmunodetection of Small Cell Lung Carcinoma (SCLC) was assessed in an experimental model. A human-derived SCLC cell line, GLC28, was xenografted in nude rats. MOC-31, a Moab (IgG1) reactive with a membrane antigen on GLC-28 cells, was labeled with different radionuclides (123I, 125I and 111In) with a labeling efficiency of 50 to 70%. Immunoreactivity of 125I- and 111In-labeled MOC-31 was retained, but a decreased immunoreactivity was found for 123I-labeled MOC-31.Biodistribution of 125I- and 111In-labeled MOC-31 after injection, showed a good localization in GLC-28 (SCLC) tumor target cells in vivo. The amount of localized IgG varied depending on the cellular composition of tumor tissue. The radioimmunodetection (gamma-camera) of GLC- 28 tumor xenografts in the flank or neck of nude rats by 111In-labeled MOC-31 was superior to 125I-labeled MOC-31.
It is concluded, MOC-31 can be radiolabeled under defined circumstances and used for immunodetection experiments of GLC-28 tumor cells in vivo.
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de Jonge, M.W.A. et al. (1990). Monoclonal Antibodies Radiolabeled with Different Radioisotopes for Biodistribution and Radioimmunodetection of Tumor Xenografts in the Nude Rat. In: Crommelin, D.J.A., Schellekens, H. (eds) From Clone to Clinic. Developments in Biotherapy, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3780-5_18
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DOI: https://doi.org/10.1007/978-94-011-3780-5_18
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