Abstract
In this paper some requirements for successful immunoscintigraphy with radiolabelled monoclonal antibodies are discussed such as appropriate radionuclides and labelling methods. We describe the labelling of an antifibrin with 99mTc and the purification procedure and quality control of this radiolabelled antibody illustrating the problems encountered in converting a highly specific monoclonal antibody into an efficacious radiopharmaceutical.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Andres, R.Y.; Schubiger, P.A. (1986) Radiolabelling of antibodies: Methods and limitations. Nucl.Med.25:162–166
Wolf, W.; Shani, J. (1986) Criteria for the selection of the most desirable radionuclide for radiolabeling monoclonal antibodies. Nucl.Med.Bio1.13:319–324
Paik, C.H.; Phan, L.N.B.; Hong, J.J.; Sahami, M.S.; Heald, S.C.; Reba, R.C.; Steigman, J.; Eckelman, W.C. (1985) The labeling of high affinity sites of antibodies with 99mTc. Nucl.Med.Bio1.12:3–8
DeNardo, S.J.; DeNardo, G.L.; Peng, J.S. (1989) Pharmaceutical quality Control of Radiolabeled Monoclonal Antibodies and their Fragments. Am.J.Phys.Imaging 4:39–44
Eckelman, W.C.; Paik, C.H. (1986) Comparison of 99mTc and 111In labeling of conjugated antibodies. Nucl.Med.Biol.13:335–343
Rhodes, B.A.; Zamora, P.O.; Newell, K.D.; Valdez, E.F. (1986) Technetium-99m labeling of murine monoclonal antibody fragments. J.Nucl.Med.27:685–693
Lanteigne, D.; Hnatowich, D.J. (1984) The labeling of DTPA-coupled proteins with 99mTc. Int.J.Appl.Radiat.Isot.35:617–621
Feitsma, R.I.J.; Blok, D.; Wasser, M.N.J.M.; Nieuwenhuizen, W.; Pauwels, E.K.J. (1987) A new method for 99mTc-labelling of proteins with an application to clot detection with an antifibrin monoclonal antibody. Nucl.Med.Commun.8:771–777
Blok, D.; Feitsma, R.I.J.; Wasser, M.N.J.M.; Nieuwenhuizen, W.; Pauwels, E.K.J. (1989) A new method for protein labeling with 99mTc. Nucl.Med.Biol.16:11–16
Lindmo, T.; Boven, E.; Cuttitta, F.; Fedorko, J.; Bunn, P.A. (1984) Determination of the Immunoreactive Fraction of Radiolabeled Monoclonal Antibodies by Linear Extrapolation to Binding at Infinite Antigen Excess. J.Immunol.Methods 72:77–89
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1990 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Blok, D., Feitsma, R.I.J., Pauwels, E.K.J. (1990). Monoclonal Antibodies in Radioimmunoscintigraphy Some hurdles between clone and clinic. In: Crommelin, D.J.A., Schellekens, H. (eds) From Clone to Clinic. Developments in Biotherapy, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3780-5_13
Download citation
DOI: https://doi.org/10.1007/978-94-011-3780-5_13
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5683-0
Online ISBN: 978-94-011-3780-5
eBook Packages: Springer Book Archive