Fluorescence microscopy

  • J. James
  • H. J. Tanke


Many dyes exhibit the phenomenon of absorption when they are exposed to light, and a consequent rise in temperature. There are also compounds that emit part of the absorbed energy as light rays. This phenomenon is called fluorescence if the emission takes place very shortly after the absorption of light; it is called phosphorescence or delayed fluorescence where the emission is significantly delayed. All these phenomena, taken together, are called luminescence. To generate luminescence an external energy source, for instance a lamp, is necessary.


Fluorescence Microscopy Numerical Aperture Excitation Light Lens System Barrier Filter 
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Recommended further reading

  1. Parker CA (1968): Photoluminescence of Solutions: With Applications to Photochemistry and Analytical Chemistry. Amsterdam, London, New York: Elsevier.Google Scholar
  2. Ploem JS, Tanke HJ (1987): Introduction to fluorescence microscopy. Roy Micr Soc Microscopy Handbooks. Oxford: Oxford University Press.Google Scholar
  3. Taylor DL, Waggoner AS, Murphy RF, Lanni F, Birge RR (1986): Applications of Fluorescence in Biomedical Sciences. New York: Alan R. Liss.Google Scholar
  4. Wick G, Baudner S, Herzog F (1978): Immunofluorescence. Marburg: Med. Verlaggesellschaft.Google Scholar

Copyright information

© Springer Science+Business Media Dordrecht 1991

Authors and Affiliations

  • J. James
    • 1
  • H. J. Tanke
    • 2
  1. 1.Faculty of MedicineUniversity of AmsterdamAmsterdamThe Netherlands
  2. 2.Faculty of MedicineUniversity of LeidenLeidenThe Netherlands

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