Skip to main content
SpringerLink
Log in

The light microscope as a tool for observation and measurement: illumination and image formation

  • Chapter
Biomedical Light Microscopy

  • 167 Accesses

Abstract

When a light wave strikes an object, different kinds of interaction may take place, resulting in a modulation of the illuminating rays. These interactions may be a factor in image formation, but they can also be a disturbing element.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Recommended further reading

  • Brakenhoff GJ, Blom P, Barends P (1979): Confocal scanning light microscopy with high aperture immersion lenses. J Microsc 117: 219–232.

    Article  Google Scholar 

  • Brakenhoff GJ, Van der Voort HTM, Van Spronsen EA, Linnemans WAM, Nanninga N (1985): Three-dimensional chromatin distribution in neuroblastoma nuclei shown by confocal scanning laser microscopy. Nature 317: 748–749.

    Article  PubMed  CAS  Google Scholar 

  • Hopkins HH, Barham PM (1950): The influence of the condensor on microscopic resolution. Proc Phys Soc 63: 737–744.

    Article  Google Scholar 

  • James J (1983): Instrumental versus human factors in microscopic discovery. Proc Roy Micr Soc 18: 361–365.

    Google Scholar 

  • Rienitz J (1983): Two-point resolution with partially coherent illumination, and the relation between self-luminous and illuminated objects. Microsc Acta 87: 311–328.

    Google Scholar 

  • Turner GLE (1967): The microscope as a technical frontier in science. In: Bradbury S, Turner GLE (eds), Historical Aspects of Microscopy. Cambridge: W Heffer & Sons, Ltd.

    Google Scholar 

  • White JG, Amos WB, Fordham M (1987): An evaluation of confocal versus conventional imaging of biological structures by fluorescence light microscopy. J Cell Biol 105: 41–48.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Faculty of Medicine, University of Amsterdam, Amsterdam, The Netherlands

    J. James (Professor of Histology)

  2. Faculty of Medicine, University of Leiden, Leiden, The Netherlands

    H. J. Tanke (Associate Professor of Cell Biology)

Authors
  1. J. James
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. J. Tanke
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

Copyright information

© 1991 Springer Science+Business Media Dordrecht

About this chapter

Cite this chapter

James, J., Tanke, H.J. (1991). The light microscope as a tool for observation and measurement: illumination and image formation. In: Biomedical Light Microscopy. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3778-2_2

Download citation

  • DOI: https://doi.org/10.1007/978-94-011-3778-2_2

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5682-3

  • Online ISBN: 978-94-011-3778-2

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation