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Equilibrium and Dynamic Investigation on the Main Phase Transition of Dipalmytoylphosphatidylcholine Vesicles Containing Polypeptides: A DSC and Iodine Laser T-Jump Study

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The Structure, Dynamics and Equilibrium Properties of Colloidal Systems

Part of the book series: NATO ASI Series ((ASIC,volume 324))

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Abstract

The influence of the linear polypeptide gramicidin A’ (GA’) on the dynamics of the main phase transition in single shell DPPC vesicles was investigated in equilibrium and kinetic measurements. Additional experiments were carried out with vesicles containing low amounts of the synthetic polypeptide Iys2–gly-leu24–lys2–ala-amide. Turbidity measurements showed a broadening of the phase transition for both polypeptides and a hysteresis in the transition curves. DSC peaks indicated that at least two types of lipids coexist for DPPC-GA’ vesicles in the concentration range 0.02 ≤ X(GA’) ≤ 0.04. The transition enthalpy was decreasing linearly for both polypeptides in the investigated concentration range. Kinetic experiments were performed on our iodine-laser T-jump arrangement (ILTJ) using turbidity detection. In the region of the main phase transition an increasing percentage of the overall turbidity change was shifted from the ms to the µs time range when increasing amounts of polypeptides were present in the phospholipid bilayer. We conclude that domains containing lipids of different order (clusters) as they are assumed to coexist in pure DPPC bilayers become much less important if polypeptides are incorporated.

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Abbreviations

DMPC:

Dimyristoylphosphatidylcholine

DPPC:

Dipalmytoyiphosphatidylcholine

GA’:

Gramicidin A’

PP:

Lys2–Gly-Leu24–Lys2–Ala-amide

ILTJ:

Iodine-Laser-Tempeiaiure-Jump

DSC:

Differential Scanning Calorimetry

NMR:

Nuclear Magnetic Resonance

ΔH:

Enthalpy of the Phase Transition

CD:

Circular Dichroism

Tm :

Temperature of the Main Phase Transition Midpoint

Ala:

Alanine

Gly:

Glycine

Lau:

Leucine

Lys:

Lysine

Trp:

Tryptothan

Val:

Valine

References

  1. R. Sages and B. Witkop, J. Am. Chem. Soc. 87, 2011–2020, (1965).

    Article  Google Scholar 

  2. R.E. Koeppe, D.A. Paczkowski and W.L. Whaley, Biochem. 24, 2822–2826 (1985).

    Article  CAS  Google Scholar 

  3. J.D. Glickson, D.F. Mayers, J.M. Settine and D.W. Urry, Biochem. 11, 477–486 (1972).

    Article  CAS  Google Scholar 

  4. D.S. Andersen, Ann. Rev. Physiol. 46, 531–548 (1984).

    Article  CAS  Google Scholar 

  5. E. Bamberg and P. Läuger, J. Membr. Biol. 11, 177 (1973).

    Article  CAS  Google Scholar 

  6. E. Bamberg and P. Läuger, BBA 367, 127–133 (1974).

    Article  CAS  Google Scholar 

  7. W.R. Veatch, R. Mathies, M. Eisenberg and L. Stryer, J. Mol. Bio. 99, 75–92 (1975).

    Article  CAS  Google Scholar 

  8. W. Veatch and L. Stryer, J. Mol. Biol. 113, 89–102 (1977).

    Article  CAS  Google Scholar 

  9. S. Weinstein, B. A. Wallace, E.R. Blout, J.S. Morrow and W. Veatch, PNAS 76, 4230–4234 (1979).

    Article  CAS  Google Scholar 

  10. D.W. Urry, PNAS 68, 672–679 (1971).

    Article  CAS  Google Scholar 

  11. S. Weinstein, B.A. Wallace, J.S. Morrow and W.R. Veatch, J. Mol. Bio. 143, 1–19 (1980).

    Article  CAS  Google Scholar 

  12. E. Bamberg and H. Janko, BA 465, 486–499 (1977).

    CAS  Google Scholar 

  13. N.R. Clement and J.M. Gold, Biochem, 20, 1544–1548 (1981).

    Article  CAS  Google Scholar 

  14. A. Finkelstein and O.S. Andersen, J. Membr. Biol. 59, 155–171 (1981).

    Article  CAS  Google Scholar 

  15. B.A. Wallace, W.R. Veatch and E.R. Blout, Biochem. 20, 5754–5760 (1981).

    Article  CAS  Google Scholar 

  16. E. Oldfield, R. Gilmore, M. Glaser, H.S. Gutowsky, J.C. Hshung, S.J. Kang, T. King, M. Meadows and R. Rice, J. Bio. Chem. 256, 1160–1166 (1978).

    Google Scholar 

  17. B.A. Cornell and M. Kenivy, BBA 732, 705–710 (1983).

    Article  CAS  Google Scholar 

  18. S. Rajan, S.Y. Kang, H.S. Gutowsky and E. Oldfield, J. Bio. Chem. 256, 1160–1166 (1981).

    CAS  Google Scholar 

  19. D. Chapman, B.A. Cornell, A.W. Eliasz and A. Perry, J. Mol. Biol. 13, 517–538 (1977).

    Article  Google Scholar 

  20. D.C. Lee, A. Aziz and D. Chapman, BBA 769, 49–56 (1984).

    Article  CAS  Google Scholar 

  21. D.A. Pink, A. Georgallas and D. Chapman, Biochem. 20, 7152–7157 (1981).

    Article  CAS  Google Scholar 

  22. J.H. Davis, D.M. Clare, R.S. Hodges and M. Bloom, Biochem. 22, 5298–5305 (1983).

    Article  CAS  Google Scholar 

  23. M.R. Morrow, J.C. Huchschilt and J.H. Davis, Biochem. 24, 5369–5406 (1985).

    Google Scholar 

  24. J.C. Huchschilt, R.S. Hodges and J.H. Davis, Biochem. 24, 1377–1386 (1985).

    Article  Google Scholar 

  25. J.F. Holzwarth, V. Eck and A. Genz, in “Spectroscopy and Dynamics of Biological Systems”, eds. P.M. Bayley and R. Dale, 351–377, Acad. Press, London (1985).

    Google Scholar 

  26. A. Genz and J.F. Holzwarth, Colloid & Polymer Sci. 263, 484–493 (1985).

    Article  CAS  Google Scholar 

  27. A. Genz and J.F. Holzwarth, Europ. Biophys. J., 13, 323 (1986).

    CAS  Google Scholar 

  28. J.F. Holzwarth, Faraday Discussion No, 81, Lipid Vesicles and Membranes, 353–366 (1986).

    Google Scholar 

  29. J.F. Holzwarth, A. Schmidt, H. Wolff and R. Volk, J. Phys. Chem. 81, 2300 (1977)

    Article  CAS  Google Scholar 

  30. J.F. Holzwarth, Laser Temperature Jump, pp. 47–76, in: Techniques and Applications of Fast Reactions in Solution, W.J. Gettins and E. Wyn-Jones, eds., D. Reidel Publ. Comp. Dordrecht, Holland (1979).

    Google Scholar 

  31. J.M. Kremer, M.W. Eskey, C. Pathmamanoharan and P.H. Wiersema, Biochem. 16, 3932–3925 (1977).

    Article  CAS  Google Scholar 

  32. B. Gruenewald, S. Stankowski and A. Blume, FEBS Lett. 102, 227 (1979).

    Article  CAS  Google Scholar 

  33. CS. Chong and K. Colbow, BBA 436, 260–282 (1976).

    Article  CAS  Google Scholar 

  34. P.W.M. Van Dijck, B. De Kruyff, P.A. Aarts, A.J. Verkleij and J. De Gier, BBA 506, 183–191 (1978).

    Article  Google Scholar 

  35. B.A. Cornell, M.M. Sacre, W.E. Peel and D. Chapman, FEBS Lett. 90, 29 (1978).

    Article  CAS  Google Scholar 

  36. F. Jähnig, Biophys. J. 36, 329–345 (1981).

    Article  Google Scholar 

  37. D. Lichtenberg, E. Freire, C.F. Schmidt, Y. Barenholz, P.L. Felgner and T.E. Thompson, Biochem. 20, 3462–3466 (1981).

    Article  CAS  Google Scholar 

  38. O.G. Mouritsen and M. Bloom, Biophys. J. 46, 141–153 (1984).

    Article  CAS  Google Scholar 

  39. B. Gruenewald, W. Frisch and J.F. Holzwarth, BBA 641, 311–319 (1981).

    Article  CAS  Google Scholar 

  40. G. Adam, Zeutschrift f. Naturforschung 23b, 181–197 (1968);

    Google Scholar 

  41. G. Adam, Cooperative Transitions in Biological Membranes, pp. 220–231, in: Synergistics: Cooperative Processes in Multi-Component Systems, H. Haken ed., B.G. Teubner Verlag, Stuttgart (1973).

    Google Scholar 

  42. M.I. Kanehisa and T.Y. Tsong, J. Am. Chem. Soc. 100, 424–432 (1978).

    Article  CAS  Google Scholar 

  43. O.G. Mourtisen, BBA 731, 217–221 (1983).

    Article  Google Scholar 

  44. O.G. Mourtisen, A. Boothroyd, R. Harris, N. Jan, T. Lookman, L. Mac Donald, D.A. Pink and M.J. Zuckermann, J. Chem. Phys. 79, 2027–2041 (1983).

    Article  Google Scholar 

  45. O.G. Mouritsen and M.J. Zuckermann, Europ. Biphys. J. 12, 75–86 (1985).

    CAS  Google Scholar 

  46. O.G. Mouritssen, Phys. Rev. B 31, 2613–2616 (1985).

    Article  Google Scholar 

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Author information

Authors and Affiliations

  1. Fritz-Haber-Institut der Max-Planck-Gesellschaft, Faradayweg 4–6, D-1000, Berlin 33, West Germany

    A. Genz & J. F. Holzwarth

  2. Department of Biochemistry, University of Minnesota, St. Paul, Minnesota, 55104, USA

    T. Y. Tsong

Authors
  1. A. Genz
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  2. T. Y. Tsong
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  3. J. F. Holzwarth
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Editor information

Editors and Affiliations

  1. Department of Chemistry and Applied Chemistry, University of Salford, Salford, UK

    D. M. Bloor  & E. Wyn-Jones  & 

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Genz, A., Tsong, T.Y., Holzwarth, J.F. (1990). Equilibrium and Dynamic Investigation on the Main Phase Transition of Dipalmytoylphosphatidylcholine Vesicles Containing Polypeptides: A DSC and Iodine Laser T-Jump Study. In: Bloor, D.M., Wyn-Jones, E. (eds) The Structure, Dynamics and Equilibrium Properties of Colloidal Systems. NATO ASI Series, vol 324. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3746-1_32

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  • DOI: https://doi.org/10.1007/978-94-011-3746-1_32

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5666-3

  • Online ISBN: 978-94-011-3746-1

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