Abstract
The development, acquisition otherwise, cryopreservation, characterization and distribution of cell lines represent the major activities of our department. For example, cell lines of fibroblastic, epithelial and lymphoblastic origin plus hybridomas have been developed. Standard and serum-free systems have been utilized. A wide variety of human and animal lines have been acquired otherwise and accessioned. The characteristics of representative cultures are discussed. Cell lines are tested routinely for microbial contaminants including mycoplasma and species is verified by isoenzymology. Karyology, the DNA fingerprint for human lines, functional traits, tumorigenicity and other characteristics are confirmed as appropriate. Computerized databases on cell lines are now available internationally for ATCC clients via CODATA and TYMNET or Telenet networks. By using the databases one can quickly retrieve information about specific lines or create lists of lines with specific characteristics.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Caputo, J.L., Hay, R.J. and Williams, C.D. (1979) ‘The isolation and properties of an epithelial cell strain from rhesus monkey bronchus’. In Vitro 15, 222–223.
Caputo, J.L., Thompson, A., McClintock, P., Reid, Y.A. and Hay, R.J. (1991) ‘An effective method for establishing human B lymphoblastic cell lines using Epstein-Barr virus’ J. Tiss. Cult. Meth. 13, 39–44.
Carney, D.N., Gazdar, A.F., Bepler, G., Guccion, J.G., Marangos, P.J. et al. (1985) ‘Establishment and identification of small cell lung cancer cell lines having classic and variant features’ Canc. Res. 45, 2913–2923.
Friedman, H.S., Burger, P.C., Bigner, S.H., Trojanowski, J.Q., Brodeur, G.M., et al. (1988) ‘Phenotypic and genotypic analyses of a human medulloblastoma cell line and transplantable xenograft (D341 Med) demonstrating amplification of c-myc’ Amer. J. Pathol. 130, 472–484.
Gazdar, A.F., Carney, D.N., Nau, M.M. and Minna, J.D. (1985) ‘Chaaracterization of variant subclasses of cell lines derived from small cell lung cancer having distinctive biochemical, morphological and growth properties’ Canc. Res. 45, 2924–2930.
Gazdar, A.F., Linnoila, R.I., Kurita, Y., Oie, H.K., Mulshine, J.L. et al. (1990) ‘Peripheral airway cell differentiation in human lung cancer cell lines’ Canc. Res. 50, 5481–5487.
Gilbert, D.A., Reid, Y.A., White, C., Hay, R.J. and O’Brien, S.J. (1990) ‘Application of DNA fingerprints for cell line individualization’. Amer. J. Hum. Gen. 47, 499–514.
Hamburger, A.W, Reid, Y.A., Pella, B., Milo, G.E., Noyes, I., Krakauer, H. and Fuhrer, J.P. (1985a) ‘Isolation and characterization of a monoclonal antibody specific for epithelial cells’ Canc. Res. 45, 783–790.
Hamburger, A.W., Reid, Y.A., Pelle, B.A., Breth, L.A., Beg, N. Ryan, U. and Cines, D.B. (1985b) ‘Isolation and characterization of monoclonal antibodies reactive with endothelial cells’ Tiss. and Cell 17, 451–459.
Hay, R.J., Williams, C.D., Macy, M.L. and Lavappa, K.S. (1982) ‘Cultured cell lines for research on pulmonary, physiology available through the American Type Culture Collection. Amer. Rev. Resp. Dis. 125, 222–232.
Hay, R.J. (1984) ‘Problems of specificity from the cell banking perspective: Colon cell lines at the American Type Culture Collection’.Prog. Cancer Res. Ther. 29, 3–21.
Hay, R.J. ‘Preservation and characterization’. Chapter 4 ed. R.I. Freshney, in Animal Cell Culture: A Practical Approach, IRL Press, Washington, D.C. pp. 71–112.
Hay, R.J. (1988) ‘The seed stock concept and quality control for cell lines’. Anal. Biochem. 171, 225–237.
Hay, R.J., Macy, M., Chen, T.R., McClintock, P. and Reid, R.A. (1988). American Type Culture Collection Catalogue of Cell Lines and Hybridomas, 6th edition, Rockville, MD.
Hay, R.J.; Macy, M., Chen, T.R. (1989), ‘Mycoplasma infection of cultured cells’. Nature 339, 487–488.
Hay, R.J., Macy, M., Chen, T.R., McClintock, P. and Reid, R.A. (1990). American Type Culture Collection Update Cell Lines and Hybridoma, Rockville, Md.
Lawlor, D.A., Ward, F.E., Ennis, P.D., Jackson, A.P. and Parham, P., (1988). ‘HLA-A and B polymorphisms predate the divergence of humans and chimpanzees’ Nature 135, 268–271.
Logsdon, C.D., Moessner, J., Williams, J.A. and Goldfine, I.D. (1985) ‘Glucocorticoids increase amylase mRNA levels, secretory organelles, and secretion in pancreatic acinar AR42J cells’ J. Cell Biol. 100, 1200–1208.
Reid, Y.A., Gilbert, D.A. and O’Brien, S.J. (1990) ‘The use of DNA hypervariable probes for human cell line identification’. ATCC Newsletter 10 (4), 1–2.
Ruoff, N.M. and Hay, R.J. ‘Metabolic and temporal studies of pancreatic exocrine colonial aggregates in vitro (1979). Cell and Tiss. Res. 204, 243–252.
Seva, C., Scemama, J.L., Bastie, M.J., Pradayrol, L., and Vayasse, N. (1990). ‘Lorglumide and loxiglumide inhibit gastrin-stimulated DNA synthesis in a rat tumoral acinar pancreatic cell line (AR42J)’ Canc. Res. 50, 5829–5833.
Thompson, A.A., Dilworth, S. and Hay, R.J. (1985) ‘Isolation and culture of colonic epithelial cells in serum-free medium’ J. Tiss. Cult. Meth. 9, 117–122.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Hay, R.J. (1991). Development, Availability and Characterization of ATCC Human and Animal Cell Lines. In: Sasaki, R., Ikura, K. (eds) Animal Cell Culture and Production of Biologicals. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3550-4_4
Download citation
DOI: https://doi.org/10.1007/978-94-011-3550-4_4
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5572-7
Online ISBN: 978-94-011-3550-4
eBook Packages: Springer Book Archive