Abstract
A cell line, designated BTS (BSC-40, temperature sensitive, SV40) has been isolated by stably transfecting BSC-40 monkey cells with the tsA58 allele of the SV40 large T antigen gene. At the nonpermissive temperature (39.5°C) SV40 origin-containing plasmids can be stably transfected to yield cells lines with chromosomal, low copy-number integrants. Upon shiftdown to the permissive temperature (33°C), the large T antigen protein becomes active, the integrated origins begin to replicate, the transfected DNA excises, and continues to replicate episomally to very high copy numbers. This expression cell system is ideally suited for research and development experiments on a pilot scale.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Blochlinger, K. and Diggelmann, H. (1984) Mol. Cell. Biol. 4: 2929–2931.
Botchan, M., Topp, W. and Sambrook, J. (1979) CHS Symp. Quant. Biol. 43: 709–719.
Brockman, W.W. and Nathans, D. (1974) Proc. Natl. Acad. Sci. USA 71: 942–946.
Gluzman, Y. (1981) Cell 23: 175–182.
Jat, P.S., Cepko, C.L., Mulligan, R.C. and Sharp, P.A. (1986) Mol. Cell. Biol. 6: 1204–1217.
Kaufman, R.D. and Sharp, P.A. (1982) J. Mol. Biol. 159: 601–621.
Kern, F.G. and Basilico, C. (1986) Gene 43: 237–245.
Portela, A., Melero, J.A., de la Luna, S. and Ortin, J. (1986) EMBO J. 5: 2387–2392.
Rio, D.C., Clark, S.G. and Tjian, R. (1986) Science 227: 23–28.
Sedivy, J.M. and Sharp, P.A. (1987) Cell 50: 379–389.
Sedivy, J.M. (1988) Bio/Technology 6: 1192–1196.
Tegtmeyer, P. (1972) J. Virol. 10: 591–602.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Sedivy, J.M. (1991). Pilot Scale Protein Production Using Inducible Gene Amplification. In: Sasaki, R., Ikura, K. (eds) Animal Cell Culture and Production of Biologicals. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3550-4_29
Download citation
DOI: https://doi.org/10.1007/978-94-011-3550-4_29
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5572-7
Online ISBN: 978-94-011-3550-4
eBook Packages: Springer Book Archive