Abstract
Based on the data from 60 days’ continuous cultivation, the effects of high molecular weight growth factors on antibody production were analyzed quantitatively. Transferrin had no effect on antibody production, while BSA enhanced the antibody production rate significantly. The antibody production rate increased 4 times and 14 times in the case of the feeding BSA of 2 g/ℓ and 5g/ℓ into the EC space (space connected to the outer part of the hollow fibers) respectively compared with the data without addition of BSA. Five g/ℓ of BSA addition into the IC space (space connected to the inner part of the hollow fibers) resulted in 2.5 times increase of this production rate compared with no addition of BSA. The antibody production rate in the hollow-fiber system was increased 3 times by BSA feeding as much as that in the perfusion culture system.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Altshuler G.L., Dziewulski D.M., Sowek J.A. and Belfort G. (1986) ‘Continuous hybridoma growth and monoclonal antibody production in hollow fiber reactors-separators’, Biotechnology and Bioengineering 28, 646–658.
Hopkinson J. (1985) ‘Hollow fiber cell culture systems for economical cell-product manufacturing’, Bio/technology 3, 225–230.
Murakami H., Masui H., Sato G.H., Sueoka N., Chow T.P. and KanoSueoka T. (1982) ‘Growth of hybridoma cells in serum-free medium: Ethanolamine is an essential component’, Proceedings of the National Academy of Sciences of the United States of America 79, 1163–1165.
Nilausen K. (1978) ‘Role of fatty acids in growth-promoting effect of serum albumin on hamster cells in vitro’, Journal of Cellular Physiology 96, 1–14.
Rockwell G.A., Sato G.H. and Mcclure D.B. (1980) ‘The growth requirements of SV40 virus transformed Balb/c-3T3 cells in serum-free monolayer culture’ Journal of Cellular Physiology 103, 323–331.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Omasa, T. et al. (1991). Hybridoma Culture in the Hollow-Fiber System -The Effects of Growth Factors-. In: Sasaki, R., Ikura, K. (eds) Animal Cell Culture and Production of Biologicals. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3550-4_26
Download citation
DOI: https://doi.org/10.1007/978-94-011-3550-4_26
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5572-7
Online ISBN: 978-94-011-3550-4
eBook Packages: Springer Book Archive