Abstract
Specific protein production of mammalian cells have been promoted by applying a low constant potential to the surface of an electrode on which cells were cultured in serum-free medium. A human carcinoma cell line, MKN45 cells, were cultured on a platinum-coated plate electrode and carbon-mesh electrode with an applied potential below 0.6 V vs. Ag/AgCl in serum-free medium. The carcinoembryonic antigen (CEA) production was dependent on the electrode potential and was rather enhanced at 0.4 V vs. Ag/AgCl on both types of electrode. This optimum potential of 0.4 V vs. Ag/AgC1 agreed with the potential that maximally promoted the CEA production in fetal bovine serum (FBS) containing medium. This phenomenon could led us to design a new type of bioreactor which would control the protein production by electrical effects.
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© 1991 Springer Science+Business Media Dordrecht
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Aizawa, M., Kojima, J., Shinohara, H., Morioka, S., Nagaike, K., Ikariyama, Y. (1991). Electrically controlled culture of MKN45 cells in serum-free medium. In: Sasaki, R., Ikura, K. (eds) Animal Cell Culture and Production of Biologicals. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3550-4_25
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DOI: https://doi.org/10.1007/978-94-011-3550-4_25
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5572-7
Online ISBN: 978-94-011-3550-4
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