Abstract
We have evaluated whether transgenic mouse technology could be used to derive immortalised recombinant cell lines stably expressing heterologous proteins, such as human al-antitrypsin and human factor IX. In this system, the expression of an onc gene and the gene of interest is targetted to a specific cell type in transgenic mice using tissue-specific regulatory DNA sequences. This leads to the generation of specific tumours: onc gene-induced lymphomas and hepatomas. The tumour cells are then cultured and permanent producer cell lines are established. Under appropriate culture conditions, several cell lines derived from trans-hepatomas maintain a differentiated phenotype, and are capable of expressing biologically active and correctly processed factor IX.
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© 1991 Springer Science+Business Media Dordrecht
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Jallat, S. et al. (1991). Transgenic Animals as a Source of Genetically-Engineered Trans-Immortalised Cell Lines. In: Sasaki, R., Ikura, K. (eds) Animal Cell Culture and Production of Biologicals. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3550-4_2
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DOI: https://doi.org/10.1007/978-94-011-3550-4_2
Publisher Name: Springer, Dordrecht
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