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Animal Cell Culture and Production of Biologicals pp 135–140Cite as

Operation of an Air Lift Reactor for Production of Immunochemicals by Immobilized Hybridoma Cells

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Abstract

The feasibility of using external loop bioreactor system for the production of monoclonal antibodies by mouse/mouse hybridoma cells immobilized in Poly-l-lysine(PLL)-alginate microcapsule was investigated. Batch culture of immobilized cells followed logarithmic growth, reaching a concentration of 7 x 107 cells/mL of capsule after 20 days giving monoclonal antobody (mAb) concentrations of up to 600.μg/ml of capsule. The main advantages of the bioreactor system used is control of process parameters, minimized shear stresses on immobilized cells and retention of the product (mAb) within the microcapsule.

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References

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Author information

Authors and Affiliations

  1. Department of Chemical Engineering, University of Belgrade, Karnegijeva 4, 11000, Belgrade, Yugoslavia

    B. Bugarski, G. Vunjak & G. Jovanovic

  2. Institute for Immunology, Endocrinology and Nutrition, INEP, Zemun, Yugoslavia

    K. Cuperlovic

  3. Department of Chemical Engineerig, Queen’s University, Kingston, K71 3N6, Canada

    M. F. Goosen

Authors
  1. B. Bugarski
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  2. G. Vunjak
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  3. G. Jovanovic
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  4. K. Cuperlovic
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  5. M. F. Goosen
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Editor information

Editors and Affiliations

  1. Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kyoto, Japan

    Ryuzo Sasaki  & Koji Ikura  & 

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© 1991 Springer Science+Business Media Dordrecht

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Bugarski, B., Vunjak, G., Jovanovic, G., Cuperlovic, K., Goosen, M.F. (1991). Operation of an Air Lift Reactor for Production of Immunochemicals by Immobilized Hybridoma Cells. In: Sasaki, R., Ikura, K. (eds) Animal Cell Culture and Production of Biologicals. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3550-4_16

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  • DOI: https://doi.org/10.1007/978-94-011-3550-4_16

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5572-7

  • Online ISBN: 978-94-011-3550-4

  • eBook Packages: Springer Book Archive

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