Abstract
A continuous bioreactor using a spinner flask equipped with cell precipitator was applied to cultivate insect cells. Maximum viable density of insect cells in this bioreactor system reached 1.38 x 106 cells/ml at the dilution rate of 0.005 hr-1. Recombinant protein production by cells infected with a genetically-modified baculovirus was also demonstrated. The maximum β-galactosidase synthesis of 7500 units per reactor volume was achieved at the dilution rate of 0.011 hr-1.
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© 1991 Springer Science+Business Media Dordrecht
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Kim, H.R., Chung, B.H., Kim, C.H., Chung, I.S. (1991). Continuous Culture with Cell Precipitation for Recombinant Protein Production. In: Sasaki, R., Ikura, K. (eds) Animal Cell Culture and Production of Biologicals. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3550-4_10
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DOI: https://doi.org/10.1007/978-94-011-3550-4_10
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5572-7
Online ISBN: 978-94-011-3550-4
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