Abstract
We have attempted to obtain a genetic map of A. vinelandii UW strain, constructing multimarked strains and using them for mapping experiments. Three mapping techniques have been employed. 1. Cotransfer of markers mediated by plasmids R68.45 and pJB3JI. 2. Isolation of R-prime derivatives of plasmid pJB3JI and analysis of markers included in the chromosomal fragment. 3. Polar transfer of chromosomal markers mediated by Tn5-Mob.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Blanco G, Ramos F, Medina JR and Tortolero M (1990). Mol. Gen. Genet (in press).
Kennedy C and Toukdarian A (1987). Ann. Rev of Microbiol. 41: 227–258.
Luque F, Santero E, Medina JR and Tortolero M (1987). Microbiologia SEM 3:45–48.
Mergeay M, Lejeune P, Sadouk A, Gerits J, Fabry J (1987). Mol. Gen. Genet: 209:61–70.
Nagpal P, Iafri S, Reddy MA, Das HK (1989) J. Bacteriol. 171: 3133–3138.
Sadoff HL, Shimel B, Ellis S (1979). J. Bacteriol. 138: 871–877.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Blanco, G., Ramos, F., Medina, J.R., Gutierrez, J.C., Tortolero, M. (1991). A Chromosomal Linkage Map of Azotobacter Vinelandii . In: Polsinelli, M., Materassi, R., Vincenzini, M. (eds) Nitrogen Fixation. Developments in Plant and Soil Sciences, vol 48. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3486-6_9
Download citation
DOI: https://doi.org/10.1007/978-94-011-3486-6_9
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5541-3
Online ISBN: 978-94-011-3486-6
eBook Packages: Springer Book Archive