Abstract
Bacteria of the genus Azospirillum are diazotrophs associated with the roots of grasses. Considerable progress towards understanding the molecular biology of their association with plants is based on the identification of plant-inducible genes. With this goal, we have isolated and sequenced a set of “constitutive” promoter-containing DNA fragments of Azospirillum brasilense Sp7 (1). Transcriptional fusions to CAT reporter gene of plasmid pKK232-8 (2) allowed us identified in E. coli-constitutive promoters at the frequence of 3.4%. The first 17 sequences, isolated at 20 μg/ml Cm, have been classified in seven MIC classes of resistance from 20 to 280 μg/ml of Cm (Fig. 1).
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© 1991 Springer Science+Business Media Dordrecht
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Civardi, L., Delledonne, M., Marudelli, M., Fogher, C. (1991). Nucleotide sequence of regulatory regions of Azospirillum brasilense.. In: Polsinelli, M., Materassi, R., Vincenzini, M. (eds) Nitrogen Fixation. Developments in Plant and Soil Sciences, vol 48. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3486-6_47
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DOI: https://doi.org/10.1007/978-94-011-3486-6_47
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