Abstract
A genomic library of Azospirillum brasilense was constructed and used to isolate the nifA gene by complementation of a nifA - mutant of A. brasilense (FP10). A recombinant plasmid, pMAK7, was isolated and found to contain a functional nifA gene and to hybridize with nifA and nifB probes from Herbaspirillum seropedicae.
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© 1991 Springer Science+Business Media Dordrecht
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Knopik, M.A., Funayama, S., Rigo, L.U., Souza, E.M., Machado, H.B., Pedrosa, F.O. (1991). Cloning of the nifA and nifB Genes of Azospirillum Brasilense Strain FP2. In: Polsinelli, M., Materassi, R., Vincenzini, M. (eds) Nitrogen Fixation. Developments in Plant and Soil Sciences, vol 48. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3486-6_25
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DOI: https://doi.org/10.1007/978-94-011-3486-6_25
Publisher Name: Springer, Dordrecht
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