Abstract
Bacillus thuringiensis var israelensis (BTI), when ingested by mosquito larvae, is highly toxic to them. Many other aquatic invertebrates feed on bacteria but, in general, BTI is not toxic to them. We tested in the laboratory the hypothesis that certain crustaceans indirectly benefit mosquito larvae by reducing mortality caused by BTI. We presumed the mechanism to be ingestion of the bacteria by the crustaceans resulting in a lower concentration available to the mosquito larvae.
Mortality of Aedes aegypti larvae exposed to BTI was reduced in the presence of the fairy shrimp, Branchipus schaefferi and the ostracod, Cypridopsis vidua (only during summer trials and not autumn trials for the latter species) but was not reduced in the presence of the ostracod, Heterocypris incongruens. By contrast, H. incongruens preyed upon infected, though still-moving, larvae. Feeding on the bacteria by the crustaceans may not be an important mechanism; our data indicates that the crustaceans did not reduce BTI in the water. Moreover, B. schaefferi, introduced into water and then removed prior to the introduction of BTI and mosquitoes, also reduced mosquito mortality.
The mechanism for the protective effect of B. schaefferi and C. vidua is unknown. However, these results suggest that the abundance of certain organisms co-occurring with mosquito larvae may partially explain why the effective concentration of BTI varies among habitats.
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References
Aly, C., 1983. Influence of feeding behavior of Aedes aegypti on the effectiveness of Bacillus thuringiensis var israelensis. Bull. Soc. Vector Ecol. 8: 94–100.
Barnes, H. G., 1974. Invertebrate Zoology. Saunders, Philadelphia, 970 pp.
Bence, J. R., 1988. Indirect effects and biological control of mosquitoes by mosquitofish. J. Appl. Ecol. 25: 505–521.
Blaustein, L., 1990. Evidence for predatory flatworms as organizers of zooplankton and mosquito community structure in rice fields. Hydrobiologia, 199: 179–191.
Blaustein, L. & R. Karban, 1990. Indirect effects of the mosquitofish, Gambusia affinis, on the mosquito, Culex tarsalis. Limnol. Oceanogr., 35: 767–771.
Cannon, H. G., 1925. On the feeding mechanism of a freshwater ostracod, Pioncypris vidua (O.F. Muller). J. linn. Soc. Lond., Zool. 36: 325–335.
Chesson, J., 1989. The effect of alternative prey on the functional response of Notonecta hoffmani. Ecology 70: 1227–1235.
Dimentman, Ch., 1976. Distribution and biology of Anostraca (Crustacea) in temporary pools in Israel and factors affecting it. PhD dissertation, Hebrew University, 140 pp.
Dimentman, Ch., 1981. The rain pool ecosystems of Israel: Geographical distribution of freshwater Anostraca (Crustacea). Israel J. Zool. 90: 1–15.
Dimentman, Ch. & J. Margalit, 1981. Rainpools as breeding and dispersal sites of mosquitoes and other aquatic insects in the Central Negev Desert. J. Arid Envir. 4: 123–129.
Diner, M. P., E. P. Odum & P. F. Hendrix, 1986. Comparisons of the roles of ostracods and cladocerans in regulating community structure and metabolism in freshwater microcosms. Hydrobiologia 133: 59–63.
Goldberg, L. J. & J. Margalit, 1976. Bacterial spore demonstrating rapid larvicidal activity against Anopheles sergentii, Uranotaenia unguiculata, Culex univittatus, Aedes aegypti and Culex pipiens. Mosq. News 37: 355–358.
Gophen, M. & W. Geller, 1974. Filter mesh size and food particle uptake by Daphnia. Oecologia 64: 408–412.
Hinman, E. H., 1930. A study of the food of mosquito larvae (Culicidae). Am. J. Hyg. 12: 238–270.
Holt, R. D., 1977. Predation, apparent competition and the structure of prey communities. Theor. Pop. Biol. 12: 197–229.
Hoy, J. B., E. E. Kauffman & A. G. O’Berg, 1972. A large scale field test of Gambusia affinis and Chlopyriphos for mosquito control. Mosq. News 32: 162–171.
Kerfoot, W. C. & A. Sih, 1987. Predation: direct and indirect effects on aquatic communities. University Press of New England, 386 pp.
Khawaled, K., Z. Barak & A. Zaritsky, 1988. Feeding behavior of Aedes aegypti larvae and toxicity of dispersed and of naturally encapsulated Bacillus thuringiensis var israelensis. J. Invertebr. Zool. 52: 419–426.
Lampert, W., 1974. A method for determining food selection by zooplankton. Limnol. Oceanogr. 19: 995–998.
Margalit, J. & A. Dean, 1985. The story of Bacillus thuringiensis var israelensis (B.t.i.). J. Am. Mosq. Control Ass. 1: 1–7.
Margalit, J., C. Pascar-Gluzman, H. Bobroglio, A. Barak, & L. Lahkim-Tsror, 1985. Biocontrol of mosquitoes in Israel. In: Laird, M. & J. W. Miles (eds), Integrated mosquito control strategies, Vol. 2, pp. 361–364. Academic Press.
Miller, J. H., 1972. Formulas and recipes. In: Miller, J. H. (ed.), ‘Experiments in molecular genetics’ Cold Spring Harbor, New York, p. 431.
Miller, T. E. & W. C. Kerfoot, 1987. Redefining indirect effects. In: Kerfoot, W. C. & A. Sih (ed.), Predation: direct and indirect effects on aquatic communities. University Press of New England, pp. 33–37.
Modlin, R. F., 1982. A comparison of two Eubranchipus species (Crustacea, Anostraca). Am. Midl. Nat. 107: 107–113.
Mulligan, F. S., C. H. Schaefer & T. Miura, 1978. Laboratory and field evaluation of Bacillus sphaericus as a mosquito control agent. J. Econ. Ent. 71: 774–778.
Paine, R. T., 1980. Food webs: linkage, interaction strength and community infrastructure. J. anim. Ecol. 49: 667–685.
Pennak, R. W., 1978. Freshwater invertebrates of the United States. 2nd edition. John Wiley & Sons, New York, 803 pp.
Ramoska, W. A. & C. Pacey, 1979. Food availability and period of exposure as factors of Bacillus sphaericus efficacy on mosquito larvae. J. Econ. Ent. 27: 523–525.
Ramoska, W. A., S. Singer & R. Levy, 1977. Bioassay of three strains of Bacillus sphaericus on field collected mosquito larvae. J. Invert. Pathol. 30: 151–154.
Rettich, F., 1986. Our experience with the use of Bacillus thuringiensis H-14 in mosquito control in Czechoslovakia. Dipterologica bohemoslovaca IV: 129–133.
Singer, S., 1973. Insecticidal activity of recent bacterial isolates and their toxins against mosquito larvae. Nature 244: 110–111.
Walker, E. D., E. J. Olds & R. W. Merritt, 1988. Gut content analysis of mosquito larvae (Diptera: Culicidae) using DAPI stain and epifluorescence microscopy. J. Med. Ent. 25: 551–554.
Weiser, J., 1976. The intermediary host for the fungus Coelomomyces chironomi. J. Invertebr. Pathol. 28: 273–274.
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© 1991 Springer Science+Business Media Dordrecht
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Blaustein, L., Margalit, J. (1991). Indirect effects of the fairy shrimp, Branchipus schaefferi and two ostracod species on Bacillus thuringiensis var Israelensis-induced mortality in mosquito larvae. In: Belk, D., Dumont, H.J., Munuswamy, N. (eds) Studies on Large Branchiopod Biology and Aquaculture. Developments in Hydrobiology, vol 64. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3366-1_9
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DOI: https://doi.org/10.1007/978-94-011-3366-1_9
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