Abstract
Growth rates of some rhizosphere bacteria can be measured by the use of 3H-thymidine (3H-TdR) labelling. However fluorescent pseudomonads of soil origin were found to incorporate little or no 3H-TdR into DNA. The conversion factor relating cellular growth rate to 3H-TdR incorporation for exponentially growing cultures of non-pseudomonads was about 2 × 1018 cells mole TdR-1. This value is comparable to constants used in aquatic environments.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Bowen G D and Rovira A D 1973 Are modelling approaches useful in rhizosphere biology? Bull. Ecol. Res. Comm. (Stockholm) 17, 443–450.
Boyce R P and Setlow R B 1962 A simple method of increasing the incorporation of thymidine into deoxyribonucleic acid of Escherichia coli. Biochim. Biophys. Acta 61, 618–620.
Christensen H, Funck-Jensen D and Kjøller A 1989 Growth rate of rhizosphere bacteria measured directly by the tritiated thymidine incorporation technique. Soil Biol. Biochem. 21, 113–117.
Grivell A R and Jackson J F 1968 Thymidine kinase: evidence for its absence from Neurospora crassa and some other micro-organisms, and the relevance of this to the specific labelling of deoxyribonucleic acid. J. Gen. Microbiol. 54, 307–317.
Herdman M 1985 The evolution of bacterial genomes. In The Evolution of Genome Size. Ed. T Cavalier-Smith, pp. 37–68. John Wiley and Sons, Ltd., New York.
Kirchman D, Ducklow H and Mitchell R 1982 Estimates of bacterial growth from changes in uptake rates and biomass. Appl. Environ. Microbiol. 44, 1296–1307.
Moriarty D J W 1986 Measurement of bacterial growth rates in aquatic systems from rates of nucleic acid synthesis. Adv. Microbiol. Ecol. 9, 245–292.
Neuhard J and Nygaard P 1987 Purines and pyrimidines. In Escherichia coli and Salmonella typhimurium. Ed. F C Neidhardt. Vol. 1. pp 445–473. American Society for Microbiology, Washington.
Riemann B and Søndergaard M 1984 Measurements of diel rates of bacterial secondary production in aquatic environments. Appl. Environ. Microbiol. 47, 632–638.
Rovira A D and Sands D C 1971 Fluorescent pseudomonads — a residual component in the soil microflora? J. Appl. Bacteriol. 34, 253–259.
Saito H, Tomioka H and Ohkido S 1985 Further studies of thymidine kinase: Distribution pattern of the enzyme in bacteria. J. Gen. Microbiol. 131, 3091–3098.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Christensen, H. (1991). Conversion factors relating thymidine uptake to growth rate with rhizosphere bacteria. In: Keister, D.L., Cregan, P.B. (eds) The Rhizosphere and Plant Growth. Beltsville Symposia in Agricultural Research, vol 14. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3336-4_11
Download citation
DOI: https://doi.org/10.1007/978-94-011-3336-4_11
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5473-7
Online ISBN: 978-94-011-3336-4
eBook Packages: Springer Book Archive