Abstract
A number of authors have reported the successful establishment of continuous cell lines from human squamous cell carcinomas from various sites (Moore et al., 1955; Eagle 1955; Giard et al., 1973; Nishihara et al., 1979; Huang et al., 1980; Easty et al., 1981a,b; Rheinwald and Beckett, 1981). Most of these methods do not permit the clonal and selective growth of the squamous cell carcinoma cells in primary culture. However, Rheinwald and Beckett, (1981) clonally cultivated human squamous cell carcinoma suspensions upon lethally irradiated feeder layers of Swiss 3T3 mouse fibroblasts and succeeded in establishing continuous cell lines from the epidermis and the tongue. They also reported that the squamous cell carcinoma cell lines were able to proliferate optimally in lower concentrations of foetal bovine serum than their normal counterparts (Rheinwald and Beckett, 1981) and that this was the only consistent difference from their normal counterparts in vitro. We have used medium supplemented with lower levels of foetal bovine serum to selectively cultivate squamous cell carcinomas from primary biopsies. The low serum concentration inhibits the proliferation of normal fibroblasts and keratinocytes, which are the major source of contamination of these tumours.
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References
Alitalo, K., Kuismanen, E., Myllyla, R., Kiistala, U., Asko-Selgavaara, S. and Vaheri, A. (1982). Extracellular matrix proteins of human epidermal keratinocytes and feeder 3T3 cells. J. Cell. Biol., 94, 497–505.
Boukamp, P., Rupniak, H.T.R. and Fusenig, N.E. (1985). Environmental modulation of the expression of differentiation and malignancy in six human squamous carcinoma cell lines. Cancer Res. 45, 5582–559
Cline, P.R. and Rice, R.H. (1983). Modulation of involucrin and envelope competence in human keratinocytes by hydrocortisone, retinyl acetate and growth arrest. Cancer Res. 43, 3203–3207.
Cowin, P. and Garrod, D.R. (1983). Antibodies to epithelial desmosomes show wide tissue and species cross-reactivity. Nature, 302, 148–150.
Cowin, P., Mattey, D. and Garrod, D. (1984). Identification of desmosomal surface components (desmocollins) and inhibition of desmosome formation by specific Fabl. J. Cell Sci. 70, 41–60.
Cowley, G., Gusterson, B. and Knight, J. (1983). Growth in agar and tumor formation in immunologically incompetent mice as criteria for keratinocyte transformation. Cancer Lett., 21, 95–104.
Eagle, H. (1955). Propagation in a fluid medium of a human epidermoid carcinoma, strain KB. Proc. Soc. Exp. Biol. Med. 89, 362–364.
Easty, D.M., Easty, G.C., Carter, R.L., Monaghan, P. and Butler, L.J. (1981a) Ten human carcinoma cell lines derived from squamous carcinomas of the head and neck. Brit. J. Cancer, 43, 772–785.
Easty, D.M., Easty, G.C., Carter, R.L., Monaghan, P., Pittam, M.R. and James, T. (1981b). Five human tumour cell lines derived from a primary squamous carcinoma of the tongue, two subsequent local recurrences and two nodal metastases. Brit. J. Cancer, 44, 363–370.
Eisinger, M. and Marko, O. (1982). Selective proliferation of normal human melanocytes in vitro in the presence of phorbol ester and choleratoxin. Proc. Natl. Acad. Sci. USA 79, 2018–2022.
Franke, W.W., Appelhans, B. and Schmid, E. (1979). Identification and characterization of epithelial cells in mammalian tissues by immunofluorescence microscopy using antibodies to prekeratin. Differentiation 15, 7–25.
Franke, W.W., Moll, R., Schiller, D.L., Schmid, E., Kartenbeck, J. and Muller, H. (1982). Desmoplakins of epithelial and myocardial desmosomes are immunologically and biochemically related. Differentiation 23, 115–127.
Franke, W.W., Schmid, E., Grund, C., Muller, H., Engelbrecht, I., Moll, R., Stadler, J. and Jarasch, E.-D. (1981). Antibodies to high molecular weight polypeptides of desmosomes: specific localisation of a class of junctional proteins in cells and tissues. Differentiation 20, 217–241.
Giard, D.J., Aaronson, S.A., Todaro, G.J., Arnstein, P., Kessey, J.H., Dosik, H. and Parks, W.P. (1973). In vitro cultivation of human tumours: Establishment of cell lines derived from a series of solid tumors. J. Natl. Cancer Inst. 51, 1417–1423.
Gill, G.N. and Lazar, C.S. (1981). Increased phosphotyrosine content and inhibition of proliferation in EGF-treated A43l cells. Nature 293, 305–307.
Green, H. (1978). Cyclic AMP in relation to proliferation of the epidermal cell: a new view. Cell 15, 801–811.
Grimwood, R.E., Johnson, C.A., Ferris, C.F., Mercill, D.B., Mellette, J.R. and Hull, J.C. (1985). Transplantation of human basal cell carcinomas to athymic mice. Cancer, 56, 519–523.
Halaban, R. and Alfano, E.D. (1984). Selective elimination of fibroblasts from cultures of normal melanocytes. In Vitro 20, 447–450.
Halaban, R., Ghosh, S. and Baird, A. (1987). bFGF is the putative natural growth factor for human melanocytes. In Vitro Cell Dev. Biol. 23, 47–52.
Halaban, R., Langdon, R., Birchall, N., Cuono, C., Baird, A., Scott, G., Moellman, G. and McGuire, J. (1988). Paracrine stimulation of melanocytes by keratinocytes through basic fibroblast growth factor. J. Cell Biol. 197, 1611–1619.
Halaban, R., Pomerantz, S.H., Marshall, S., Lambert, D.T. and Lerner, A.B. (1983). Regulation of tyrosinase in human melanocytes grown in culture. J Cell Physiol., 97, 480–488.
Hernandez, A.D., Hibbs, M.S. and Postlethwaite, A.E. (1985). Establishment of basal cell carcinoma in culture: Evidence for a basal cell carcinoma-derived factor(s) which stimulates fibroblasts to proliferate and release collagenase. J. Invest. Dermatol. 85, 470–475.
Huang, D.P., Ho, J.H.C., Chen, E.C., Saw, D., Lui, M., Li, C.L., Mak, L.S., Lai, S.H. and Poon, Y.F. (1980). Establishment of a cell line (NPC/HKL) from a differentiated squamous cell carcinoma of the nasopharynx. Int. J. Cancer 26, 127–132.
Lane, E.B. (1982). Monoclonal antibodies provide specific molecular markers for the study of epithelial tonofilament organization. J. Cell Biol. 92, 665–673.
Mancianti, M.-L. and Herlyn, M. (1989). Tumor progression in melanoma: The biology of epidermal melanocytes in vitro. In: C.J. Conti, T.G. Slaga and A.J.P. Klein-Szanto (eds)’ Carcinogenesis. A Comprehensive Survey. Vol 11, Skin Tumours Experimental and Clinical Aspects’ New York, Raven Press pp 369–383.
Moore, A.E., Sabachewsky, L. and Toolan, M.W. (1955). Culture characteristics of four permanent lines of human cancer cells. Cancer Res. 15, 598–602.
Nishihira, T., Kasai, M., Mon, S., Watanabe, T., Kuriya, Y, Suda, M., Kitamura, M., Hirayama, K, Akaishi, T. and Sasaki, T. (1979). Characteristics of two cell lines (TE-1 and TE-2) derived from human squamous cell carcinomas of esophagus. Gann, 70, 575–584.
Parkinson, E.K., Grabham, P. and Emmerson, A. (1983). A subpopulation of cultured human keratinocytes which is resistant to the induction of terminal differentiation-related changes by phorbol 12-myristate, 13-acetate: evidence for an increase in the resistant population following transformation. Carcinogenesis 4, 857–861.
Parkinson, E.K. and Newbold, R.F. (1980). Benzo(a)pyrene metabolism and DNA adduct formation in serially cultivated strains of human epidermal keratinocytes. Int. J. Cancer 26, 289–299.
Pera, M.F. and Gorman, P.A. (1984). In vitro analysis of multistage carcinogenesis: development of indefinite renewal capacity and reduced growth factor requirements in colony forming keratinocytes precedes malignant transformation. Carcinogenesis 5, 671–682.
Rheinwald, J.G. (1979). The role of terminal differentiation in the finite culture lifetime of the human epidermal keratinocyte. Int. Rev. Cytol. Suppl. 10, 25–33.
Rheinwald, J.G. (1980). Serial cultivation of normal human epidermal keratinocytes. Methods Cell Biol. 21A, 229–254.
Rheinwald, J.G. and Beckett, M.A. (1980). Defective terminal differentiaton in culture as a consistent and selectable character of malignant human keratinocytes. Cell 22, 629–632.
Rheinwald, J.G. and Beckett, M.A. (1981). Tumorigenic keratinocyte lines requiring anchorage and fibroblast support cultured from human squamous cell carcinomas. Cancer Res. 41, 1657–1663.
Rheinwald, J.G. and Green, H. (1975). Serial cultivation of strains of human epidermal keratinocytes: The formation of keratinizing colonies from single cells. Cell 6, 331–342.
Rheinwald, J.G. and Green, H. (1977). Epidermal growth factor and the multiplication of cultured human epidermal keratinocytes. Nature 265, 421–424.
Rice, R.H. and Green, H. (1979). Presence in human epidermal cells of a soluble protein precursor of the cross-linked envelopes. Activation of the cross-linking by calcium ions. Cell 18, 681–694.
Rice, R.H. and Thacher, S.M. (1986) Involucrin: a constituent of cross-linked envelopes and marker of squamous maturation. In: J. Bereiter-Hahn, A.G. Maltoltsy and K.S. Richard (eds). Biology of the Integument 2. Springer-Verlag, pp 752–761.
Shipley, G.D., Pittelkow, M.R., Wille, J.J. Jr., Scott, R.E. and Moses, H.L. (1986). Reversible inhibition of normal human prokeratinocyte proliferation by type beta transforming growth factor/growth inhibitor in serum-free medium. Cancer Res. 46, 2068–2071.
Sun, T.-T., Shih, C. and Green, H. (1979). Keratin cytoskeleton of epithelial cells of internal organs. Proc. Natl. Acad. Sci. USA 76, 2813–2817.
Watt, F.M. and Green, H. (1981). Involucrin synthesis is correlated with cell size in human epidermal cultures. J. Cell Biol. 90, 738–742.
Woodcock-Mitchell, J., Eichner, R., Nelson, W.G. and Sun, T.-T. (1982). Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J. Cell Biol. 95, 580–588.
Yaar, M., Karassik, R.L., Schipper, L.E. and Gilchrist, B.A. (1985) Effects of alpha and beta interferons on cultured human keratinocytes. J. Invest Dermatol. 85, 70–74.
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Parkinson, E.K., Yeudall, W.A. (1991). Epidermis. In: Masters, J.R.W. (eds) Human Cancer in Primary Culture, A Handbook. Developments in Oncology, vol 64. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3304-3_8
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DOI: https://doi.org/10.1007/978-94-011-3304-3_8
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