Abstract
A number of authors have reported the successful establishment of continuous cell lines from human squamous cell carcinomas from various sites (Moore et al., 1955; Eagle 1955; Giard et al., 1973; Nishihara et al., 1979; Huang et al., 1980; Easty et al., 1981a,b; Rheinwald and Beckett, 1981). Most of these methods do not permit the clonal and selective growth of the squamous cell carcinoma cells in primary culture. However, Rheinwald and Beckett, (1981) clonally cultivated human squamous cell carcinoma suspensions upon lethally irradiated feeder layers of Swiss 3T3 mouse fibroblasts and succeeded in establishing continuous cell lines from the epidermis and the tongue. They also reported that the squamous cell carcinoma cell lines were able to proliferate optimally in lower concentrations of foetal bovine serum than their normal counterparts (Rheinwald and Beckett, 1981) and that this was the only consistent difference from their normal counterparts in vitro. We have used medium supplemented with lower levels of foetal bovine serum to selectively cultivate squamous cell carcinomas from primary biopsies. The low serum concentration inhibits the proliferation of normal fibroblasts and keratinocytes, which are the major source of contamination of these tumours.
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Parkinson, E.K., Yeudall, W.A. (1991). Epidermis. In: Masters, J.R.W. (eds) Human Cancer in Primary Culture, A Handbook. Developments in Oncology, vol 64. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3304-3_8
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DOI: https://doi.org/10.1007/978-94-011-3304-3_8
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