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The requirement of chrysobactin dependent iron transport for virulence incited by Erwinia chrysanthemi on Saintpaulia ionantha

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Iron Nutrition and Interactions in Plants

Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 43))

Abstract

To incite a systemic disease on its specific host, Saintpaulia ionantha, the soft-rot Erwinia chrysanthemi strain 3937 requires a functional high affinity iron transport system. Under iron starvation, strain 3937 produces chrysobactin, a novel catechol-type siderophore. Recent advances in the biochemistry and genetics of iron assimilation in E. chrysanthemi are reported. Analysis of leaf intercellular fluid from healthy and infected plants suggests: (i) leaf vessels in which the bacteria develop during infection would be low in free iron and (ii) chrysobactin could be produced in planta.

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References

  • Arnow L E 1937 Colorimetrie determination of the components of 3,4-dihydroxyphenylalanine-tyrosine mixtures. J. Biol. Chem. 118, 531–537.

    CAS  Google Scholar 

  • Bagg A and Neilands J B 1987 Molecular mechanism of regulation of siderophore mediated iron assimilation. Microbiol. Rev. 51, 509–518.

    PubMed  CAS  Google Scholar 

  • Boccara M and Chatain V 1989 Regulation and role in pathogenicity of Erwinia chrysanthemi 3937 pectin methylesterase. J. Bacteriol. 171, 4085–4087.

    PubMed  CAS  Google Scholar 

  • Boccara M, Diolez A, Rouve M and Kotoujansky A 1988 The role of individual pectate lyases of Erwinia chrysanthemi strain 3937 in pathogenicity on saintpaulia plants. Physiol. Mol. Plant Pathol. 33, 95–104.

    Article  CAS  Google Scholar 

  • Bolin C A and Jensen A E 1987 Passive immunization with antibodies against iron-regulated outer membrane proteins protects turkeys from Escherichia coli septicemia. Infect. Immun. 55, 1239–1242.

    PubMed  CAS  Google Scholar 

  • Brown J C 1978 Mechanism of iron uptake by plants. Plant Cell Environ. 1, 249–157.

    Article  Google Scholar 

  • Carter P 1971 Spectrophotometric determination of serum iron at the submicrogram level with a new reagent (ferrozine). Anal. Biochem. 40, 450–458.

    Article  PubMed  CAS  Google Scholar 

  • Clewell D B and Helinski D R 1969 Supercoiled circular DNA-protein complex in Escherichia coli: Purification and induced conversion to an open circular form. Proc. Natl. Acad. Sci. USA 62, 1159–1166.

    Article  PubMed  CAS  Google Scholar 

  • Collmer A and Keen N T 1986 The role of pectic enzymes in plant pathogenesis. Annu. Rev. Phytopathol. 24, 383–409.

    Article  CAS  Google Scholar 

  • De Lorenzo V, Giovannini F, Herrero M and Neilands J B 1988 Metal ion regulation of gene expression: Fur repressor-operator interaction at the promoter region of the aerobactin system of pColV-K30. J. Mol. Biol. 203, 875–884.

    Article  PubMed  Google Scholar 

  • De Witt P J G M and Spikman G 1982 Evidence for the occurrence of race and cultivar-specific elicitors of necrosis in intercellular fluids of compatible interactions of Clados-porium fulvum and tomato. Physiol. Plant Pathol. 21, 1–11.

    Article  Google Scholar 

  • Ditta G, Stanfield S, Corbin D and Helinski D R 1980 Broad hot range DNA cloning system for gram-negative bacteria: Construction of a gene bank of Rhizobium meliloti. Proc. Natl. Acad. Sci. USA 77, 7347–7351.

    Article  CAS  Google Scholar 

  • Enard C, Diolez A and Expert D 1988 Systemic virulence of Erwinia chrysanthemi 3937 requires a functional iron assimilation system. J. Bacteriol. 170, 2419–2426.

    PubMed  CAS  Google Scholar 

  • Expert D and Toussaint A 1985 Bacteriocin-resistant mutants of Erwinia chrysanthemi: Possible involvement of iron acquisition in phytopathogenicity. J. Bacteriol. 163, 221–227.

    PubMed  CAS  Google Scholar 

  • Gill P R and Warren G J 1988 An iron-antagonized fungistatic agent that is not required for iron assimilation from a fluorescent rhizosphere Pseudomonad. J. Bacteriol. 170, 163–170.

    PubMed  CAS  Google Scholar 

  • Hager D A and Burgess R R 1980 Elution of proteins from sodium dodecyl sulfate Polyacrylamide gels, removal of sodium dodecyl sulfate and renaturation of enzymatic activity: Results with Sigma subunit of Escherichia coli RNA polymerase, wheat germ DNA topoisomerase and other enzymes. Anal. Biochem. 109, 76–86.

    Article  PubMed  CAS  Google Scholar 

  • Hohn B and Collins J 1980. A small cosmid for efficient cloning of large DNA fragments. Gene 11, 291–298.

    Article  PubMed  CAS  Google Scholar 

  • Klotz L and Zimm B H 1972 Size of DNA determined by viscoelastic measurements: Results on bacteriophages, Bacillus subtilis and Escherichia coli. J. Mol. Biol. 72, 779–800.

    Article  PubMed  CAS  Google Scholar 

  • Kotoujansky A 1987 Molecular genetics of pathogenesis by soft-rot erwinias. Annu. Rev. Phytopathol. 25, 405–430.

    Article  CAS  Google Scholar 

  • Maniatis T E, Fritsch E F and Sambrook J 1982 Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 545 p.

    Google Scholar 

  • Miller J H 1972 Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

    Google Scholar 

  • Miller J F, Mekalanos J J and Falkow S 1989 Coordinate regulation and sensory transduction in the control of bacterial virulence. Science 243, 916–922.

    Article  PubMed  CAS  Google Scholar 

  • Nahlik M S, Fleming T P and Mcintosh M A 1987 Cluster of genes controlling synthesis and activation of 2,3-dihydroxybenzoic acid in production of enterobactin in Escherichia coli. J. Bacteriol. 169, 4163–4170.

    PubMed  CAS  Google Scholar 

  • O’Connor C G and Ashman L K 1982 J. Immunol. Methods 54, 267.

    Article  CAS  Google Scholar 

  • Ozenberger B A, Brickman T J and Mcintosh M A 1989 Nucleotide sequence of Escherichia coli isochorismate synthetase gene entC and evolutionary relationship of isochorismate synthetase and other chorismate-utilizing enzymes. J. Bacteriol. 171, 775–783.

    PubMed  CAS  Google Scholar 

  • Persmark M, Expert D and Neilands J B 1988 Isolation, characterization and synthesis of chrysobactin, a compound with siderophore activity from Erwinia chrysanthemi. J. Biol. Chem. 264, 3187–3193.

    Google Scholar 

  • Reverchon S and Robert-Baudouy J 1987 Regulation of expression of pectate lyase genes pelA, pelD and pelE in Erwinia chrysanthemi. J. Bacteriol. 169, 2417–2423.

    PubMed  CAS  Google Scholar 

  • Schoonejans E, Expert D and Toussaint A 1987 Characterization and virulence properties of Erwinia chrysanthemi lipopolysaccharide-defective, PhiEC2-resistant mutants. J. Bacteriol. 169, 4011–4017.

    PubMed  CAS  Google Scholar 

  • Schwyn B and Neilands J B 1987 Universal chemical assay for detection and determination of siderophores. Anal. Biochem. 160, 160, 47–56.

    Article  Google Scholar 

  • Southern E M 1975 Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503–517.

    Article  PubMed  CAS  Google Scholar 

  • Towbin H, Staehelin T and Gordon J 1979 Electrophoretic transfer of proteins from Polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. USA 76, 4350–4354.

    Article  PubMed  CAS  Google Scholar 

  • Van Gijsegem F, Toussaint A and Casadaban M 1987 Mu as a genetic tool. In Phage Mu. Eds. N Symonds, A Toussaint, P van de Putte and M M Howe, pp 246–247. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.

    Google Scholar 

  • Weinberg E D 1984 Iron withholding: A defence against infection disease. Physiol. Rev. 64, 65–102.

    PubMed  CAS  Google Scholar 

  • Williams P H 1979 Novel iron uptake system specified by ColV plasmids: An important component in the virulence of invasive strains of Escherichia coli. Infect. Immun. 26, 925–932.

    PubMed  CAS  Google Scholar 

  • Winkelmann G, Van der Helm D and Neilands J B 1987 Iron transport in microbes, plants and animals. VCH Verlagsgesellschaft mbH, Weinheim, FRG, 533 p.

    Google Scholar 

  • Young I G, Langman L, Luke R K J and Gibson F 1971 Biosynthesis of the iron transport compounds enterochelin: Mutants of Escherichia coli unable to synthetize 2,3-dihy-droxybenzoate. J. Bacteriol. 106, 51–57.

    PubMed  CAS  Google Scholar 

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© 1991 Springer Science+Business Media Dordrecht

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Enard, C. et al. (1991). The requirement of chrysobactin dependent iron transport for virulence incited by Erwinia chrysanthemi on Saintpaulia ionantha . In: Chen, Y., Hadar, Y. (eds) Iron Nutrition and Interactions in Plants. Developments in Plant and Soil Sciences, vol 43. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3294-7_37

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  • DOI: https://doi.org/10.1007/978-94-011-3294-7_37

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5455-3

  • Online ISBN: 978-94-011-3294-7

  • eBook Packages: Springer Book Archive

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