Abstract
Certain root-colonizing fluorescent pseudomonads have been shown to promote plant growth and prevent plant disease in part through the production of siderophores. However, these favorable results have not been reproduced consistently from the laboratory to the greenhouse or from the greenhouse to the field. In some circumstances siderophores appear to play no role in disease prevention. In order to understand the dynamics of competition for iron in the rhizosphere it is essential that the localization and concentration of siderophores produced by both biocontrol agents and plant pathogens be determined. We have produced monoclonal antibodies (MAbs) to ferric pseudobactin, the siderophore of plant growth-promoting Pseudomonas B10. Three IgG1 MAbs cross-react with certain ferric pseudobactins but not with others. A competitive ELISA has been developed to detect and quantify ferric pseudobactin.
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References
Bossier P and Verstraete W 1986 Detection of siderophores in soil by a direct bioassay. Soil Biol. Biochem. 18, 481–486.
Buyer J S, Sikora L J and Chaney R L 1989 A new growth medium for the study of siderophore-mediated interactions. Biol. Fertil. Soils 8, 97–101.
Buyer J S, Wright J M and Leong J 1986 Structure of Pseudobactin A214, a siderophore from a bean-deleterious Pseudomonas. Biochemistry 25, 5492–5499.
Demange P, Abdallah M and Frank H 1988 Assignment of the configurations of the amino acids in peptidic siderophores. J. Chromatography 438, 291–297.
Demange P, Wendenbaum S, Bateman A, Dell A and Abdallah M 1987 Bacterial siderophores: Structure of pyoverdins and related compounds. In Iron Transport in Microbes, Plants and Animals. Eds G Winkelmann, D van der Helm and J B Neilands. pp 167–187. V.C.H., Weinheim, FRG.
Engvall E 1980 Enzyme Immunoassay ELISA and EMIT. Meth. Enzymol. 70, 419–439.
Gill P R and Warren G J 1988 An iron-antagonized fungistatic agent that is not required for iron assimilation from a fluorescent rhizosphere pseudomonad. J. Bacteriol. 170, 163–170.
Kloepper J W, Leong J, Teintze M and Schroth M N 1980a Pseudomonas siderophores: A mechanism explaining disease-suppressive soils. Curr. Microbiol. 4, 317–320.
Kloepper J W, Leong J, Teintze M and Schroth M N 1980b Enhanced plant growth by siderophores produced by plant growth-promoting rhizobacteria. Nature 286, 885–886.
Leong J 1986 Siderophores: Their biochemistry and possible role in the biocontrol of plant pathogens. Annu. Rev. Phytopathol. 24, 187–209.
Neilands J B 1981 Microbial iron compounds. Annu. Rev. Biochem. 50, 715–731.
Neilands J B and Leong S A 1986 Siderophores in relation to plant growth and disease. Annu. Rev. Plant Physiol. 37, 187–208.
Nelson M, Cooper C R, Crowley D E, Reid C P P and Szaniszlo P J 1988 An Escherichia coli bioassay of individual siderophores in soil. J. Plant Nutr. 11, 915–924.
Powell P E, Cline G R, Reid C P P and Szaniszlo P J 1980 Occurrence of hydroxamate siderophores in soil. Nature 287, 833–834.
Powell P E, Szaniszlo P J and Reid C P P 1983 Confirmation of occurrence of hydroxamate siderophores in soil by a novel Escherichia coli bioassay. Appl. Environ. Microbiol. 46, 1080–1083.
Schippers B, Bakker A W and Bakker P A H M 1987 Interactions of deleterious and beneficial rhizosphere microorganisms and the effect of cropping practices. Annu. Rev. Phytopathol. 25, 339–358.
Teintze M, Hossain M B, Barnes C L, Leong J and Van der Helm D 1981 Structure of ferric pseudobactin, a siderophore from a plant growth promoting Pseudomonas. Biochemistry 20, 6446–6457.
Thomashow L S and Weiler D M 1988 Role of a phenazine antibiotic from Pseudomonas fluorescens in biological control of Gaeumannomyces graminis var. tritici. J. Bacteriol. 170, 3499–3508.
Yang C-C and Leong J 1984 Structure of Pseudobactin 7SR1, a siderophore from a plant-deleterious Pseudomonas. Biochemistry 23, 3534–3540.
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© 1991 Springer Science+Business Media Dordrecht
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Buyer, J.S., Sikora, L.J., Kratzke, M.G. (1991). Development of a detection system for ferric pseudobactin using monoclonal antibodies. In: Chen, Y., Hadar, Y. (eds) Iron Nutrition and Interactions in Plants. Developments in Plant and Soil Sciences, vol 43. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3294-7_34
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DOI: https://doi.org/10.1007/978-94-011-3294-7_34
Publisher Name: Springer, Dordrecht
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