Abstract
We have analyzed the ability of single site insertion mutants of Rhizobium meliloti 1021 defective in various components of a high-affinity iron transport system to produce nodules, fix nitogen and promote plant growth. Our results indicate that a high-affinity iron transport system may significantly increase the ability of the differentiated form of the bacterium to fix nitrogen and induce an increase in plant growth.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Abbreviations
- EDDA:
-
ethylenediamine-N,N’-bis(2-hydroxyphenylacetic acid)
- CAS:
-
chrome azurol S
References
Braun V, Hantke K, Eick-Helmerick K, Koster W, Preßler U, Sauer M, Schäffer S, Schöffler H, Staudenmaier H and Zimmermann L 1987 Iron transport in Escherichia coli. In Iron Transport in Microbes, Plants and Animals. Eds. G D Winkelmann, D Van der Helm and J B Neilands. pp 35–51. VCH Verlagsgesellschaft mbH, Weinheim, FRG.
Buyer J S and Leong J 1986 Iron transport-mediated antagonism between plant growth-promoting and plantdeleterious Pseudomonas strains. J. Biol. Chem. 261, 791–794.
Djordjevic M A, Gabriel D W and Rolfe B G 1987 Rhizobium — the refined parasite of legumes. Annual Rev. Phytopathol. 25, 145–168.
Enard C, Diolez A and Expert D 1988 Systemic virulence of Erwinia chrysanthemi 3937 requires a functional iron assimilation system. J. Bacteriol. 170, 2419–2426.
Gill P R and Neilands J B 1989 Cloning of a genomic region required for a high-affinity iron uptake system from Rhizobium meliloti. Molecular Microbiol. 3, 1183–1189.
Hardy R W F, Holsten R S, Jackson E K and Burns R C 1968 The acetylene-ethylene assay for N2: fixation, laboratory and field evaluations. Plant Physiol. 43, 1185–1207.
Magazin M D, Moores J C and Leong J 1986 Cloning of the gene coding for the outer membrane receptor protein for ferric pseudobactin, a siderophore from a plant growthpromoting Pseudomonas strain. J. Biol. Chem. 261, 795–799.
Meade H M, Long S R, Ruvkun G B, Brown S E and Ausubel F M 1982 Physical and genetic characterization of symbiotic and auxotrophic mutants induced by transposon Tn5 mutagenesis. J. Bacteriol. 149, 114–122.
Neilands J B 1982 Microbial envelope proteins related to iron. Annu. Rev. Microbiol. 36, 285–309.
Neilands J B 1984 Siderophores of bacteria and fungi. Microbiol. Sci. 1, 9–14.
O’Hara G W, Dilworth M J, Boonkerd N and Parkpian P 1988 Iron-deficiency specifically limits nodule development in peanut inoculated with Bradyrhizobium sp. New Phytol. 108, 51–57.
Schwyn B and Neilands J B 1987a Universal chemical assay for the detection and determination of siderophores. Anal. Biochem. 72, 248–254.
Schwyn B and Neilands J B 1987b Siderophores from agronomically important species of the Rhizobiacae. Comm. Agric. Food Chem. 1, 95–113.
Simon R, Priefer U and Puhler A 1983 A broad host range mobilization system for in vivo genetic engineering: Transposon mutagenesis in gram negative bacteria. Bio/Technology 1, 784–791.
Smith M J, Shoolery J N, Schwyn B, Holden I and Neilands J B 1985 Rhizobactin, a structurally novel siderophore from Rhizobium meliloti. J. Am. Chem. Soc. 107, 1739–1743.
Romheld V 1987 Existance of two strategies for the acquisition of iron in higher plants. In Iron Transport in Microbes, Plants and Animals. Eds. G D Winkelmann, D Van der Helm and J B Neilands. pp 353–374. VCH Verlagsgesellschaft mbH, Weinheim, FRG.
Walsh C 1979 Enzymatic Reaction Mechanisms. W H Freeman and Company, San Francisco, CA. 439 p.
Weinberg E D 1984 Iron witholding: a defense against infection disease. Physiol. Rev. 64, 65–102.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Gill, P.R., Barton, L.L., Scoble, M.D., Neilands, J.B. (1991). A high-affinity iron transport system of Rhizobium meliloti may be required for efficient nitrogen fixation in planta . In: Chen, Y., Hadar, Y. (eds) Iron Nutrition and Interactions in Plants. Developments in Plant and Soil Sciences, vol 43. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3294-7_30
Download citation
DOI: https://doi.org/10.1007/978-94-011-3294-7_30
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5455-3
Online ISBN: 978-94-011-3294-7
eBook Packages: Springer Book Archive