Abstract
In the present study, a monoclonal antibody (HK-249) that recognizes a glucosamine sulfate alpha 1→4 glucuronic acid-containing determinant in heparan sulfate (HS) chains of a basement membrane derived heparan sulfate proteoglycan was used to identify and localize HS in Downs syndrome (DS) brain, and to determine the sequence of events preceding the formation and accumulation of: fibrillar amyloid in DS. Analysis of DS patients at different ages revealed that HS accumulated within neurons of the hippocampus and amygdala, as early as 1 day after birth. Young age-matched controls did not demonstrate similar positive HS immunoreactivity in neurons whereas positive immunostaining for HS was observed in other regions thought to normally contain HS. The earliest deposition of betaamyloid protein (BAP) was first observed as “amorphous” or “diffuse” cortical deposits in DS brain in patients aged 18 and 24, prior to the accumulation of fibrillar amyloid (observed in DS patients 35 years and older). These cortical deposits also contained positive HS immunoreactivity implying that HS accumulation in conjunction with the BAP is an early event preceding the first appearance of fibrillar amyloid.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
Similar content being viewed by others
References
Snow, A.D., Wilimer, J., Kisilevsky, R. (1987) ‘Sulfated glycosaminoglycans in Alzheimer’s disease’, Lab Invest, 18, 506–510.
Snow, A.D., Lara, S., Nochlin, D., Wight, T.N. (1989) ‘Cationic dyes reveal proteoglycans structurally integrated within the characteristic lesions of Alzheimer’s disease’, Acta Neuropath, 78, 113–123.
Snow, A.D., Wight, T.N. (1989) ‘Proteoglycans in the pathogenesis of Alzheimer’s disease and other amyloidoses’, Neurobiol Aging, 10, 481–497.
Snow, A.D., Mar, H., Nochlin, D., Kimata, K., Kato, M., Suzuki, S., Hassell, J., Wight, T.N. (1988) ‘The presence of heparan sulfate proteoglycans in the neuritic plaques and congophilic angiopathy in Alzheimer’s disease’, Am J Path, 133, 456–463.
Kato, Y., Kato, M., Suzuki, S., Kimata, K. (1989) ‘A monoclonal antibody against the heparan sulfate of EHS-tumor proteoglycan’. IX International Symposium on Glycoconjugates, B8. abstract.
Puchtler, H., Sweat, F., Levine, M. (1962) ‘On the binding of congo red by amyloid’, J Histochem Cytochem, 10, 355–364.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1991 Springer Science+Business Media Dordrecht
About this chapter
Cite this chapter
Sncw, A.D. (1991). Heparan Sulfate Accumulation in the Beta-Amyloid Protein Containing Lesions of Down’s Syndrome Occurs Prior to the Appearance of Fibrillar Amyloid. In: Natvig, J.B., et al. Amyloid and Amyloidosis 1990. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3284-8_82
Download citation
DOI: https://doi.org/10.1007/978-94-011-3284-8_82
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5450-8
Online ISBN: 978-94-011-3284-8
eBook Packages: Springer Book Archive