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Amyloid and Amyloidosis 1990 pp 181–184Cite as

Complete Amino-Acid Sequence of AL-Lambda 1.1 Bence-Jones Protein EZI

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Abstract

The complete amino-acid sequence of the AL Bence-Jones protein was determined from amyloidosis patient EZI. The protein was isolated from the urine by ammoniumsulfate precipitation, ion exchange chromatography, reduced, aminoethylated or carboxymethylated, and digested with either trypsin or staphylococcal protease. The peptides were separated using RP-HPLC and characterized by amino-acid sequence analysis and amino-acid analysis. The EZI polypeptide consists of 216 amino acids. It has a N-terminus blocked by PCA and amino-acid residues specific for the lambda 1.1 subgroup. Wit Ala, Ser, Ser, Thr, and Arg in pos. 112, 114, 163, and 190 the constant part belongs to the K(-), Oz(-), Mcg (-) isotype. An alanine was identified in position 90. This replaces the hydrophilic threonine or serine residues determined in the other lambda 1.1 L-chains and represents the only hydrophilic/hydrophobic substitution.

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Author information

Authors and Affiliations

  1. Max-Planck-Institute for Experimental Medicine, Hermann-Rein-Str. 3, D-3400, Göttingen, Germany

    H. D. Kratzin, M. Stangel & N. Hilschmann

  2. Beilinson Med. Center, Petah Tikva, Tel Aviv University, Tel Aviv, Israel

    A. I. Pick

Authors
  1. H. D. Kratzin
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  2. A. I. Pick
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  3. M. Stangel
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  4. N. Hilschmann
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Editor information

Editors and Affiliations

  1. Institute of Immunology and Rheumatology, University of Oslo, Oslo, Norway

    Jacob B. Natvig

  2. Oslo Sanitetsforenings, Rheumatism Hospital, University of Oslo, Oslo, Norway

    Øystein Førre

  3. Department of Rheumatology, University of Tromsø, Tromsø, Norway

    Gunnar Husby

  4. Department of Immunology and Bloodbank, University of Tromsø, Tromsø, Norway

    Anne Husebekk  & Bjørn Skogen  & 

  5. Department of Biochemistry, University of Oslo, Oslo, Norway

    Knut Sletten

  6. Department of Pathology, University of Linköping, Linköping, Sweden

    Per Westermark

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© 1991 Springer Science+Business Media Dordrecht

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Kratzin, H.D., Pick, A.I., Stangel, M., Hilschmann, N. (1991). Complete Amino-Acid Sequence of AL-Lambda 1.1 Bence-Jones Protein EZI. In: Natvig, J.B., et al. Amyloid and Amyloidosis 1990. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3284-8_45

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  • DOI: https://doi.org/10.1007/978-94-011-3284-8_45

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5450-8

  • Online ISBN: 978-94-011-3284-8

  • eBook Packages: Springer Book Archive

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