Abstract
Polyclonal antibodies against purified mouse AA and a synthetic peptide of the SAA2 C-terminal 20 amino acids were made in rabbits. The anti-AA serum reacted with both AA and SAA, but the anti-peptide serum reacted with SAA only. Interestingly, anti -peptide serum stained AA deposits, indicating the presence of either intact SAA or partially degraded SAA. Moreover, it stained predominantly the rims of AA deposits. In contrast, homogeneous stainings of AA deposits were obtained with anti-AA serum. Our results provided visual evidence that AA deposits contain both SAA and AA and that these SAA molecules are processed, in situ, into AA as the maturation of AA fibril progresses.
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© 1991 Springer Science+Business Media Dordrecht
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Ju, S.T., Shirahama, T., Miura, K., Cohen, A.S. (1991). Generation and Use of Site-Specific Antibodies Against SAA.. In: Natvig, J.B., et al. Amyloid and Amyloidosis 1990. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3284-8_20
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DOI: https://doi.org/10.1007/978-94-011-3284-8_20
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5450-8
Online ISBN: 978-94-011-3284-8
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