Abstract
Isolated tentacles from mature scyphistomae of North Atlantic Aurelia aurita consistently regenerate complete polyps. Polyp formation occurs precipitously 6–12 w after isolation. Synchronous, accelerated regeneration may be achieved by a 12–18 h exposure to 58 mM CsCl. Precursor incorporation and inhibitor studies reveal a requirement for DNA synthesis/cell division to effect polyp formation. Continuous incubation of tentacles in hydroxyurea (5–10 mM) or aphidicolin (15–30 µM) prevents polyp formation and inhibits 3H-thymidine incorporation into DNA. Upon removal from a reversible inhibitor tentacles initiate DNA synthesis and transform into normal polyps. Tentacles placed into hydroxyurea immediately following CsCl exposure do not exhibit precocious morphogenesis. A role for gating mechanisms and cell division is discussed.
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© 1991 Springer Science+Business Media Dordrecht
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Lesh-Laurie, G.E., Hujer, A., Suchy, P. (1991). Polyp regeneration from isolated tentacles of Aurelia scyphistomae: a role for gating mechanisms and cell division. In: Williams, R.B., Cornelius, P.F.S., Hughes, R.G., Robson, E.A. (eds) Coelenterate Biology: Recent Research on Cnidaria and Ctenophora. Developments in Hydrobiology, vol 66. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-3240-4_13
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DOI: https://doi.org/10.1007/978-94-011-3240-4_13
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