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In situ hybridization for molecular cytogenetics

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In Situ Hybridization: Medical Applications

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Abstract

After the biochemical description of the principles of nucleic acid hybridization in the sixties, three groups reported independently in 1969–1970 its application for the microscopic detection of specific nucleic acid sequences (Pardue and Gall, 1969; John et al., 1969; Buongiorno-Nordelli and Amaldi, 1970). In contrast to the first microscopic work done with fluorescent antibody probes, the labels were radioisotopes, the detection of which was accomplished by micro-autoradiography. The disadvantages inherent to the use of radioisotopes (poor topological resolution, environmental and health hazards, complex multiple sequence detection) prompted several groups to develop non-isotopic nucleic acid detection techniques.

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Author information

Authors and Affiliations

  1. Department of Cytochemistry and Cytometry, Medical Faculty, Leiden University, Wassenaarseweg 72, 2333 AL, Leiden, The Netherlands

    A. K. Raap

  2. Department of Pathology, Medical Faculty, Leiden University, Wassenaarseweg 62, 2333 AL, Leiden, The Netherlands

    C. J. Cornelisse

Authors
  1. A. K. Raap
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  2. C. J. Cornelisse
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Editor information

Editors and Affiliations

  1. Department of Biochemistry, Charing Cross and Westminster Medical School, London, UK

    G. R. Coulton  & J. de Belleroche  & 

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© 1992 Springer Science+Business Media Dordrecht

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Raap, A.K., Cornelisse, C.J. (1992). In situ hybridization for molecular cytogenetics. In: Coulton, G.R., de Belleroche, J. (eds) In Situ Hybridization: Medical Applications. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2984-8_5

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  • DOI: https://doi.org/10.1007/978-94-011-2984-8_5

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-5326-6

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