Abstract
We report here the molecular and biochemical characterization of voltage-dependent sodium channels in two Drosophila melanogaster strains. One, Tübingen, is sensitive to pyrethroids such as deltamethrin and the other one, Tübingen DDT, is less susceptible to the “knock down” effect of those insecticides (kdr). We have found that the resistance of the Tübingen DDT flies is not due to a difference of metabolism of the pyrethroids nor to a difference in the sodium channel density between the two strains. On the contrary, binding experiments indicate that the apparent affinity of deltamethrin is shifted to lower values in the Tübingen DDT flies. Polyclonal antibodies directed against the two proteins which are supposed to act as sodium channels in Drosophila were used in western blotting experiments and labelled one glycoprotein of Mr≈270,000 on membranes of both Tübingen and Tübingen DDT strains. Partial sequencing of one of the putative sodium channel genes has revealed one mutation in the Tübingen DDT sequence that might be involved in the kd resistance.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Catterall, W.A., Molecular properties of voltage-sensitive sodium channels. Ann. Rev. Biochem., 1986, 55, 953–85.
Sawicki, R.M., Resistance to pyrethroid insecticides in arthropods. In Progress in Pesticide Biochemistry and Toxicology, volume 5: Insecticides, ed. D.H. Hutson & T.R. Roberts, John Wiley & Sons, Chichester, 1985, pp. 143–92.
Lund, A.E. and Narahashi, T. Kinetics of sodium channel modification by the insecticide tetramethrin in squid axon membranes. J. Pharmacol. Exp. Therap., 1981, 219, 464–73.
Vijverberg, H.P.M., Van der Zalm, J.M. and Van der Bercken, J. Similar mode of action of pyrethroids and DDT on sodium channel gating in myelinated nerves. Nature, 1982, 295, 601–3.
Scott, J. G., Pyrethroid insecticides. In ISI Atlas of Science: Pharmacology, 1988, pp. 125–8.
Jacques, Y., Romey, G., Cavey, M.T., Kartalovski, B. and Lazdunski, M. Interaction of pyrethroids with the Na+ channel in mammalian neuronal cells in culture. Biochem. Biophys. Acta, 1980, 600, 882–97.
Holan, G., Frelin, C. and Lazdunski, M. Selectivity of action between pyrethroids and combined DDT-pyrethroid insecticides on Na+ influx into mammalian neuroblastoma. In Experientia 41, Birkhauser Verlag, Basel, 1985, pp. 520–2.
Brown, G.B., Gaupp, J.E. and Olsen, R.W. Pyrethroid insecticides: Stereospecific allosteric interaction with the batrachotoxinin-A benzoate binding site of mammalian voltage-sensitive sodium channels. Mol. Pharmacol., 1988, 34, 54–9.
Lombet, A., Mourre, C. and Lazdunski, M. Interaction of insecticides of the pyrethroid family with specific binding sites on the voltage-dependent sodium channel from mammalian brain. Brain Res., 1988, 459, 44–53.
Pauron, D., Barhanin, J., Amichot, M., Pralavorio, M., Berge, J.B. and Lazdunski, M. Pyrethroid receptor in the insect Na+ channel: Alteration of its properties in pyrethroid-resistant flies. Biochemistry. 1989. 28. 1673–1677.
Cuany, A., Pralavorio, M., Pauron, D., Berge, J.B., Fournier, D., Blais, C., Lafont, R., Salaün, J.P., Weissbart, D., Larroque, C. and Lange, R. Characterization of microsomal oxidative activities in a wild-type and in a DDT resistant strain of Drosophila melanogaster. Pestic. Biochem. Physiol., 1990, 37, 293–302.
Salkoff, L., Butler, A., Wei, A., Scavarda, N., Giffen, K., Ifune, C., Goodman, R., and Mandel, G. Genomic organization and deduced amino acid sequence of a putative sodium channel gene in Drosophila. Science, 1987, 237, 744–9.
Ramaswami, M., and Tanouye, M.A. Two sodium-channel genes in Drosophila Implications for channel diversity. Proc. Natl. Acad. Sci. USA, 1989, 86, 2079–82.
Stanley, K.K., and Luzio, J.P. Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins. EMBO J., 1984, 3, 1429–34.
Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 1970, 227, 680–5.
Conyers, S.M. and Kidwell, D.A. Chromogenic substrates for horseradish peroxidase. Anal. Biochem., 1991, 192, 207–11.
Rossignol, D.P. Reduction in number of nerve membrane sodium channels in pyrethroid resistant house flies. Pestic. Biochem. Physiol., 1988, 32, 146–52.
Salkoff, L., Butler, A., Scavarda, N., and Wei, A. Nucleotide sequence of the putative sodium channel gene from Drosophila: the four homologous domains. Nucl. Acid Res., 1987, 15, 8569–72.
Gordon, D., Moskowitz, H., and Zlotkin, E. Sodium channel polypeptides in central nervous systems of various insects identified with site directed antibodies. Biochim. Biophys. Acta, 1990, 1026, 80–86.
Suzuki, D.T., Grigliatti, T. and Williamson, R. Temperaturesensitive mutants in Drosophila melanogaster: a mutation (paratS) causing reversible adult paralysis. Proc. Natl. Acad. Sci. USA, 1971, 68, 890–3.
Yool, A.J. and Schwarz, T.L. Alteration of ionic selectivity of a K+ channel by mutation in the H5 region. Nature, 1991, 349, 700–4.
Yellen, G., Jurman, M.E., Abramson, T. and MacKinnon, R. Mutations affecting internal TEA blockade identify the probable pore-forming region of a K+ channel. Science, 1991, 251, 939–42.
Hartmann, H.A., Kirsch, G.E., Drewe, J.A., Taglialatela, M., Joho, R.H. and Brown, A.M. Exchange of conduction pathways between two related K+ channels. Science, 1991, 251, 942–4.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1992 SCI
About this chapter
Cite this chapter
Pauron, D., Amichot, M., Berge, JB. (1992). Voltage-Dependent Sodium Channels in Susceptible and Pyrethroid-Resistant Drosophila Strains. In: Denholm, I., Devonshire, A.L., Hollomon, D.W. (eds) Resistance ’91: Achievements and Developments in Combating Pesticide Resistance. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2862-9_19
Download citation
DOI: https://doi.org/10.1007/978-94-011-2862-9_19
Publisher Name: Springer, Dordrecht
Print ISBN: 978-1-85166-886-1
Online ISBN: 978-94-011-2862-9
eBook Packages: Springer Book Archive