Abstract
In flow cytometry, light scattering and fluorescence of individual particles in suspension is measured at high speed. When applied to planktonic particles, the light scattering and (auto-)fluorescence properties of algal cells can be used for cell identification and counting. Analysis of the wide size spectrum of phytoplankton species, generally present in eutrophic inland and coastal waters, requires flow cytometers specially designed for this purpose. This paper compares the performance in phytoplankton research of a commercial flow cytometer to a purpose built instrument. It reports on the identification of phytoplankton and indicates an area where flow cytometry may supersede more conventional techniques: the analysis of morphological and physiological characteristics of subpopulations in phytoplankton samples.
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References
Balfoort H. W., J. Snoek, J. R. M. Smits, L. W. Breedveld, J. W. Hofstraat & J. Ringelberg, Automatic identification of algae: neural network analysis of flow cytometric data. J. of Plankton Res. 14 no. 4 (in press).
Cunningham, A., 1990. A low-cost, portable flow cytometer specifically designed for phytoplankton analysis. J. Plankton Res. 12: 149–160.
Dubelaar, G. B. J., A. C. Groenewegen, W. Stokdijk, G. J. Van den Engh & J. W. M. Visser, 1989. Optical plankton analyser: A flow cytometer for plankton analysis, II: Specifications. Cytometry 10: 529–539.
Frankel, D. S., R. J. Olson, S. L. Frankel & S. W. Chisholm, 1989. Use of a neural net computer system for analysis of flow cytometric data of phytoplankton populations. Cytometry 10: 540–550.
Golueke, C. G. & W. J. Oswald, 1965. J. Wat. Pollut. Cont. Fed. 37: 471.
Groenewegen, A. C., G. B. J. Dubelaar & J. W. M. Visser, 1991. Improved particle length measurements in the optical plankton analyser. Cytometry supplement 5. Abstracts to the 15th congress of the Soc. for Anal. Cytology. Bergen, Norway.
Kroon, B. M. A., M. Latasa, B. Ibelings & L. R. Mur, 1992. The effect of dynamic light regimes on Chlorella. Hydrobiologia 238: 71–78.
Legendre, L. & C. M. Yentsch, 1989. Overview of flow cytometry and image analysis in biological oceanography and limnology. Cytometry 10: 501–510.
Olson, R. J., S. L. Frankel & S. W. Chisholm, 1983. An inexpensive flow cytometer for the analysis of fluorescence signals in phytoplankton: chlorophyll and DNA distributions. J. exp. mar. Biol. Ecol. 68: 129–144.
Peeters, J. C. H., G. B. J. Dubelaar, J. Ringelberg & J. W. M. Visser, 1989. Optical plankton analyser: A flow cytometer for plankton analysis, I: Design considerations. Cytometry 10: 522–528.
Phinney, D. A. & T. L. Cucci, 1989. Flow cytometry and phytoplankton. Cytometry 10: 511–521.
Schuessler, R. G., 1967. An investigation of the chemical floc-culation and autoflocculation of algae. M.Sc. Thesis, University of Notre Dame, South Bend IN.
Sosik, H. M., S. W. Chisholm & R. J. Olson, 1989. Chlorophyll fluorescence from single cells: Interpretation of flow cytometric signals. Limnol. Oceanogr. 34: 1749-1761.
Stecn, H. B. & T. Lindmo, 1988. Applications of a new flow cytometer. International Clinical Products Review, March/ April, 1988.
Sukenik, A. & G. Shelef, 1984. Biotechnol. Bioeng. 26 (142).
Trask, B. J., G. J. Van den Engh & J. H. B. W. Elgershuizen, 1982. Analysis of phytoplankton by flow cytometry. Cytometry 2 No. 4: 258–264.
Yentsch, C. M., P. K. Horan, K. Muirhead, Q. Dortch, E. Haugen, L. Legendre, L. S. Murphy, M. J. Perry, D. A. Phinney, S. A. Pomponi, R. W. Spinrad, M. Wood, C. S. Yentsch & B. J. Zahuranec, 1983. Flow cytometry and cell sorting: A technique for analysis and sorting of aquatic particles. Limnol. Oceanogr. 28: 1275–1280.
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© 1992 Kluwer Academic Publishers
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Balfoort, H.W., Berman, T., Maestrini, S.Y., Wenzel, A., Zohary, T. (1992). Flow cytometry: instrumentation and application in phytoplankton research. In: Berman, T., Gons, H.J., Mur, L.R. (eds) The Daily Growth Cycle of Phytoplankton. Developments in Hydrobiology, vol 76. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2805-6_7
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DOI: https://doi.org/10.1007/978-94-011-2805-6_7
Publisher Name: Springer, Dordrecht
Print ISBN: 978-94-010-5246-7
Online ISBN: 978-94-011-2805-6
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