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Stoichiometry of Proton Uptake by Thylakoids During Electron Transport in Chloroplasts

  • Chapter
Photosynthesis: Photoreactions to Plant Productivity

Abstract

The results of measurements of proton-electron stoichiometry in chloroplast thylakoid membrane were examined. Various methods used for the measurement have been scrutinized. The stoichiometry detennined by different methods, at different stages of proton accumulation by thylakoids has been analysed. Taking this point into account as well as the values of stoichiometry expected due to operation of Q-cycle, the results obtained by flash and continuous illumination have been examined. Special atention has been paid to the effect of potential difference across the thylakoid membrane on the estimation of stoichiometry. Based on the results of the stoichiometry measurements, and on the functioning of b6 f complex. It is proposed that the conditions facilitating the Q cycle operation depend on the (1) frequent excitation of photosystems and (2) on the extent of plastoquinone pool reduction. It is concluded on the basis of experimental data that under physiological conditions at saturating light intensity, the H+/e- ratio in photosynthetic electron transport chain of higher plants is 3. The significance of this stoichiometry for satisfying the energy requirements during photosynthetic carbon reduction is emphasized.

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Authors and Affiliations

  1. Institute of Soil Science and Photosynthesis, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russia

    Boris N. Ivanov

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  1. Boris N. Ivanov
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  1. Indian Agricultural Research Institute, New Delhi, India

    Yash Pal Abrol

  2. Jawaharlal Nehru University, New Delhi, India

    Prasanna Mohanty

  3. University of Illinois, Urbana, Illinois, USA

    Govindjee

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Ivanov, B.N. (1993). Stoichiometry of Proton Uptake by Thylakoids During Electron Transport in Chloroplasts. In: Abrol, Y.P., Mohanty, P., Govindjee (eds) Photosynthesis: Photoreactions to Plant Productivity. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-2708-0_4

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